Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEPM1_RS14035 Genome accession   NZ_CP168737
Coordinates   3015516..3017243 (+) Length   575 a.a.
NCBI ID   WP_071089729.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN3149     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3010516..3022243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM1_RS14005 (ACEPM1_14005) obgE 3010570..3011670 (-) 1101 WP_197343283.1 GTPase ObgE -
  ACEPM1_RS14010 (ACEPM1_14010) rpmA 3011847..3012107 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACEPM1_RS14015 (ACEPM1_14015) rplU 3012145..3012456 (-) 312 WP_011002728.1 50S ribosomal protein L21 -
  ACEPM1_RS14020 (ACEPM1_14020) ispB 3012735..3013703 (+) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  ACEPM1_RS14030 (ACEPM1_14030) - 3013991..3015268 (+) 1278 WP_165591155.1 HlyC/CorC family transporter -
  ACEPM1_RS14035 (ACEPM1_14035) pilF 3015516..3017243 (+) 1728 WP_071089729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPM1_RS14040 (ACEPM1_14040) pilC 3017288..3018553 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  ACEPM1_RS14045 (ACEPM1_14045) - 3018556..3019437 (+) 882 WP_029240316.1 A24 family peptidase -
  ACEPM1_RS14050 (ACEPM1_14050) coaE 3019450..3020064 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  ACEPM1_RS14055 (ACEPM1_14055) zapD 3020182..3020940 (+) 759 WP_197343282.1 cell division protein ZapD -
  ACEPM1_RS14060 (ACEPM1_14060) yacG 3020951..3021166 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  ACEPM1_RS14065 (ACEPM1_14065) - 3021159..3021620 (-) 462 WP_011002737.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63738.56 Da        Isoelectric Point: 7.1546

>NTDB_id=1045436 ACEPM1_RS14035 WP_071089729.1 3015516..3017243(+) (pilF) [Ralstonia pseudosolanacearum strain RUN3149]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQEATQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1045436 ACEPM1_RS14035 WP_071089729.1 3015516..3017243(+) (pilF) [Ralstonia pseudosolanacearum strain RUN3149]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAT
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCAGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATTTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCACTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGATCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGTCGAGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.148

93.913

0.499
  pilB Acinetobacter baumannii D1279779

49.738

99.652

0.496
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.964

96.696

0.435
  pilB Vibrio campbellii strain DS40M4

44.649

94.261

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilB/pilB1 Synechocystis sp. PCC 6803

35.574

100

0.377
  pilF Thermus thermophilus HB27

38.434

97.739

0.376

Multiple sequence alignment