Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEPM1_RS02795 Genome accession   NZ_CP168737
Coordinates   588287..588796 (+) Length   169 a.a.
NCBI ID   WP_020831292.1    Uniprot ID   A0A454TT91
Organism   Ralstonia pseudosolanacearum strain RUN3149     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 583287..593796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM1_RS02770 (ACEPM1_02770) - 583632..584258 (+) 627 WP_028852631.1 DUF2889 domain-containing protein -
  ACEPM1_RS02775 (ACEPM1_02775) sucC 584325..585491 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  ACEPM1_RS02780 (ACEPM1_02780) sucD 585576..586457 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  ACEPM1_RS02785 (ACEPM1_02785) - 586607..587305 (+) 699 WP_197343116.1 TerC family protein -
  ACEPM1_RS02790 (ACEPM1_02790) pilA 587499..588020 (+) 522 WP_028852632.1 pilin Machinery gene
  ACEPM1_RS02795 (ACEPM1_02795) pilA 588287..588796 (+) 510 WP_020831292.1 pilin Machinery gene
  ACEPM1_RS02800 (ACEPM1_02800) - 588873..590606 (+) 1734 WP_028852633.1 PglL family O-oligosaccharyltransferase -
  ACEPM1_RS02805 (ACEPM1_02805) moaC 590836..591312 (-) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACEPM1_RS02810 (ACEPM1_02810) - 591406..593094 (+) 1689 WP_197343117.1 M48 family metallopeptidase -
  ACEPM1_RS02815 (ACEPM1_02815) - 593109..593696 (-) 588 WP_020831295.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17122.83 Da        Isoelectric Point: 9.9491

>NTDB_id=1045425 ACEPM1_RS02795 WP_020831292.1 588287..588796(+) (pilA) [Ralstonia pseudosolanacearum strain RUN3149]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQTDLSVGSSVFT
PTKNVAGLAIDGTTTGVPGQITITYTQAAGGGTLALVPTAAGAKLPTSSAPSGPIMWTCYAAGKAQAASSVAPNATMSLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=1045425 ACEPM1_RS02795 WP_020831292.1 588287..588796(+) (pilA) [Ralstonia pseudosolanacearum strain RUN3149]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCGCTGGTGTCGGAAAACGCTGCCAATGCACAGACTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCAGGCCTTGCAATTGACGGTACTACGACTGGTGTTCCGGGCCAGATCACCATCACCTATACGCA
GGCTGCCGGCGGCGGCACGCTGGCTCTGGTCCCGACCGCGGCAGGCGCTAAGCTGCCGACGAGTTCGGCTCCGTCGGGTC
CGATCATGTGGACCTGCTACGCTGCGGGTAAGGCACAGGCTGCCAGCAGCGTTGCTCCGAACGCTACGATGTCGCTGGCA
GCGAAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-169)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A454TT91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

91.124

100

0.911
  pilA2 Legionella pneumophila str. Paris

51.235

95.858

0.491
  pilA2 Legionella pneumophila strain ERS1305867

50.617

95.858

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.444
  comP Acinetobacter baylyi ADP1

43.023

100

0.438
  pilA/pilA1 Eikenella corrodens VA1

38.506

100

0.396

Multiple sequence alignment