Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACEN8Q_RS21335 Genome accession   NZ_CP168522
Coordinates   4710115..4712184 (-) Length   689 a.a.
NCBI ID   WP_413703706.1    Uniprot ID   -
Organism   Pseudomonas sp. Pseusp16 strain CT11-16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4705115..4717184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN8Q_RS21320 (ACEN8Q_21310) - 4706729..4708330 (-) 1602 WP_413703705.1 AAA family ATPase -
  ACEN8Q_RS21325 (ACEN8Q_21315) aroB 4708341..4709441 (-) 1101 WP_007989324.1 3-dehydroquinate synthase -
  ACEN8Q_RS21330 (ACEN8Q_21320) aroK 4709592..4710110 (-) 519 WP_015093141.1 shikimate kinase AroK -
  ACEN8Q_RS21335 (ACEN8Q_21325) pilQ 4710115..4712184 (-) 2070 WP_413703706.1 type IV pilus secretin PilQ Machinery gene
  ACEN8Q_RS21340 (ACEN8Q_21330) - 4712198..4712725 (-) 528 WP_413703707.1 pilus assembly protein PilP -
  ACEN8Q_RS21345 (ACEN8Q_21335) pilO 4712722..4713345 (-) 624 WP_413703708.1 type 4a pilus biogenesis protein PilO -
  ACEN8Q_RS21350 (ACEN8Q_21340) - 4713342..4713908 (-) 567 WP_413428445.1 PilN domain-containing protein -
  ACEN8Q_RS21355 (ACEN8Q_21345) comM 4713908..4714972 (-) 1065 WP_413703709.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74262.36 Da        Isoelectric Point: 5.5391

>NTDB_id=1044457 ACEN8Q_RS21335 WP_413703706.1 4710115..4712184(-) (pilQ) [Pseudomonas sp. Pseusp16 strain CT11-16]
MNRIFAAFGLSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDEPPPVPHGYTTESPARIALDLPGVASRLASKTRD
LGSGNARSATVVEAGDRTRLIINLAQPAPYDSRIEGNNLLVVVGQGVKPSASRSAATAMPVPARAAVTTGKSIRGVDFQR
GTQGEGNVVIDLSDPAMAPDIQEREGKIIVGFAKTRLPEPLRVRLDVKDFATPVQFVNASASGDRAIISIEPGGAFDYST
YQADSKLTISVRPATVDNLQKRNAGQHAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQA
LDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKSDERG
SITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGVNGS
STIGTPGSTSTNSPFVDMGAANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEA
SSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDFLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQS
KVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=1044457 ACEN8Q_RS21335 WP_413703706.1 4710115..4712184(-) (pilQ) [Pseudomonas sp. Pseusp16 strain CT11-16]
ATGAACAGGATTTTCGCAGCCTTCGGCCTTTCGCTATGGATAGCGTTGTTGTCGCCGATGGTACAAGCGGCCAATCTCAA
GGCGCTGGATGTCGCTGCGCTGCCGGGAGACCGTGTCGAGTTGAAGTTGTCGTTCGATGAGCCGCCCCCGGTGCCCCACG
GCTACACGACGGAATCACCAGCCCGGATTGCGCTGGATTTGCCCGGTGTCGCCAGTCGGTTGGCCAGCAAGACTCGCGAT
CTCGGCAGTGGAAATGCCCGTAGTGCAACGGTGGTGGAAGCCGGAGACCGTACGCGATTGATCATCAACCTGGCCCAGCC
GGCCCCGTACGACTCGCGAATTGAAGGCAACAATTTGCTGGTGGTAGTCGGGCAGGGTGTCAAGCCGTCGGCATCCAGGT
CGGCCGCCACGGCAATGCCGGTTCCGGCGCGAGCCGCAGTAACCACAGGCAAGTCCATTCGGGGTGTGGATTTTCAGCGA
GGCACACAGGGCGAAGGCAATGTGGTGATCGACTTGTCGGATCCAGCGATGGCGCCCGATATACAAGAGCGCGAGGGCAA
GATCATCGTCGGGTTTGCCAAAACCCGATTGCCTGAGCCGTTGCGCGTGCGTCTGGATGTGAAGGATTTCGCCACACCGG
TGCAGTTCGTCAATGCCAGCGCGAGTGGAGATCGAGCCATCATCAGCATTGAGCCTGGTGGCGCGTTCGATTACTCGACC
TATCAGGCCGACAGCAAGCTTACGATCAGCGTTCGCCCGGCGACGGTCGACAACCTGCAAAAGCGCAATGCCGGGCAGCA
TGCCTACAGCGGCGAGAAGCTTTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAACTGATCGCCGATTTCA
CCAACCTCAATCTGGTGGCCAGCGACACTGTGCAGGGTGGCATCACGTTGCGCTTGCAGAACGTGCCTTGGGATCAGGCG
CTGGATCTGGTGCTGAAAACCAAGGGGCTGGATAAGCGCAAGGTCGGCAATGTCCTGCTTGTTGCGCCGGCCGATGAAAT
TGCTGCCCGTGAGCGTCAGGAGCTGGAATCACAGAAGCAGATTGCCGATCTGGCGCCGTTGCGTCGGGAACTGTTGCAGG
TGAATTACGCCAAGGCGGCGGACATCGCCAAGCTCTTCCAGTCGGTGACCAGTGCCGAAGCGAAATCCGACGAACGGGGG
TCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTTGACGAGCTGCGCAGGATCGT
CACACAGCTGGATATCCCTGTGCGTCAGGTCATGATCGAGGCCCGGATCGTCGAAGCCAACGTCGACTATGACAAAAGCC
TCGGGGTGCGCTGGGGTGGTTCGATACAGAACAAGGGTAACTGGAACACCTCCGGCGTCAGCAATGGGGTCAATGGGTCG
TCGACCATTGGCACGCCCGGCAGTACCAGCACCAATTCACCGTTCGTCGACATGGGCGCGGCCAATAACACCTCGGGAAT
CGGCATCGCGTTCATCACCGATAACGTCTTGCTGGACCTGGAGCTGACGGCGATGGAAAAAACCGGCAACGGCGAAATCG
TCTCGCAACCCAAGGTGGTCACGTCCGACAAGGAGACCGCCAAAATCCTCAAGGGCACCGAGATTCCCTATCAGGAAGCC
AGTTCCAGTGGTGCGACTTCGGTGTCGTTCAAGGAAGCTTCGCTGTCACTGGAAGTGACACCGCAGATCACGCCCGATAA
CCGGATCATCATGGAGGTCAAGGTGACCAAGGACGAACCGGACTTCCTGAACAAGGTCCAGGATGTGCCGCCCATCAAGA
AAAACGAAGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGCGGCGTTTTCTCAAATACTCAGAGC
AAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGAGAA
AAAATCCGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)

Secretin and TonB N terminus short domain (STN)

(297-343)

Bacterial type II and III secretion system protein (Secretin)

(524-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.711

100

0.736
  pilQ Acinetobacter baumannii D1279779

38.778

100

0.424
  pilQ Acinetobacter baumannii strain A118

38.778

100

0.424
  comQ Acinetobacter baylyi ADP1

41.185

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.166

98.113

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.336

97.678

0.374

Multiple sequence alignment