Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABNX08_RS18765 Genome accession   NZ_CP168265
Coordinates   4016381..4018105 (+) Length   574 a.a.
NCBI ID   WP_349607170.1    Uniprot ID   -
Organism   Cupriavidus sp. DF5525 strain 73-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4011381..4023105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNX08_RS18735 (ABNX08_018735) obgE 4012321..4013418 (-) 1098 WP_022536659.1 GTPase ObgE -
  ABNX08_RS18740 (ABNX08_018740) rpmA 4013628..4013888 (-) 261 WP_011299102.1 50S ribosomal protein L27 -
  ABNX08_RS18745 (ABNX08_018745) rplU 4013928..4014239 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  ABNX08_RS18750 (ABNX08_018750) ispB 4014770..4015699 (+) 930 WP_051369817.1 octaprenyl diphosphate synthase -
  ABNX08_RS18760 (ABNX08_018760) - 4016034..4016174 (-) 141 WP_374063626.1 hypothetical protein -
  ABNX08_RS18765 (ABNX08_018765) pilB 4016381..4018105 (+) 1725 WP_349607170.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNX08_RS18770 (ABNX08_018770) - 4018175..4019446 (+) 1272 WP_022536664.1 type II secretion system F family protein -
  ABNX08_RS18775 (ABNX08_018775) pilD 4019450..4020349 (+) 900 WP_022536665.1 A24 family peptidase Machinery gene
  ABNX08_RS18780 (ABNX08_018780) coaE 4020436..4021074 (+) 639 WP_022536666.1 dephospho-CoA kinase -
  ABNX08_RS18785 (ABNX08_018785) zapD 4021322..4022080 (+) 759 WP_022536667.1 cell division protein ZapD -
  ABNX08_RS18790 (ABNX08_018790) - 4022111..4022302 (+) 192 WP_022536668.1 DNA gyrase inhibitor YacG -
  ABNX08_RS18795 (ABNX08_018795) - 4022481..4022843 (-) 363 WP_022536669.1 OPT/YSL family transporter -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63222.90 Da        Isoelectric Point: 7.5301

>NTDB_id=1043564 ABNX08_RS18765 WP_349607170.1 4016381..4018105(+) (pilB) [Cupriavidus sp. DF5525 strain 73-1]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLAQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVLATSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQVERKLIEYDP
VAAASAQRNRTAAEAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLLSLPKDKDAKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQLGYEPEQKALLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKRQGDLPHQALL
DAGFREQDIDGSWQPFHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARKDGVLSLRDAGLLKVKQ
GVTSLEEVLATTNI

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1043564 ABNX08_RS18765 WP_349607170.1 4016381..4018105(+) (pilB) [Cupriavidus sp. DF5525 strain 73-1]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCGCGCGA
AAAGCAGTCGCAGCTGATCGACGAAATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTCGCGGCGGACA
AGTACCAGCTGCCGCTGCTGGACCTGGCGCAGTACAACCTCAACAAGGTGCCGCCGGCGCTGGCCGGCAACCGTGAGTTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCAACATCCGACCCGTCCAACCAGGCCGG
GCTGGATGCGATCAAGGAAAAGTACAAACTGCCGGTCGAAGCGGTGGTGGTTGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACGCTGAAGAACATCTCGCCGGTACAGGTCGAGCGCAAGCTGATCGAGTACGACCCG
GTAGCCGCCGCCAGCGCCCAGCGCAACCGCACTGCCGCCGAGGCCATCGACGACGCCCCGGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAGGCCTTCCATCGCGGTGCGTCCGACCTGCACTTCGAGCCGTTCGAAACCTTCTACCGTATCCGCTTTC
GCGTGGACGGGGTGCTGCAGGAAGTCGCGCGCCCGCCGCTGGATATCCGCGACAAGATCGCTACCCGCATCAAGGTGCTG
TCGCGGCTGGATATCTCTGAAAAGCGCGTGCCGCAGGATGGCCGCATGAAGCTGCTGCTCTCGCTACCCAAGGACAAGGA
CGCCAAGGAGACGGTCGAGAAGGCGGTGGACTTCCGCGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGC
GGATTCTCGAATCGTCATCCGACAAGCTCGACATCGACCAACTCGGCTACGAGCCGGAGCAGAAGGCACTGCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCAGCGGCAAGACAGTGTCGCTGTACACCTTTCT
GAACCTGCTGAACCAGGGCGATATCAATATCTCCACTGCGGAGGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAATGACAAGGCCGGGCTGACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGTGACCTGGAAACCGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACGCGGCTGATGAACATGGGCGTGGCGCCCTTCAACATCGCGTCAAGCGTGC
TGATGATCACCGCGCAGCGGCTGGCACGCCGGCTGTGCACCTGCAAGCGCCAGGGCGATTTGCCACACCAGGCGCTGCTC
GACGCCGGGTTCCGCGAGCAGGATATCGACGGCAGCTGGCAGCCCTTCCATCCGGTCGGATGCGAGCGCTGCAACGGCAG
CGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCATGCCGATCACGGAGGCCATGCAGCAGATCATCCTGTCGCACGGCA
CGGCCTTGCAAATCGCGGAGCAGGCGCGCAAGGACGGCGTGCTATCGTTGCGCGACGCGGGGCTGCTGAAGGTAAAGCAG
GGCGTCACGTCACTCGAAGAAGTGCTGGCGACCACGAATATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(187-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.439

100

0.524
  pilF Neisseria gonorrhoeae MS11

51.295

100

0.517
  pilB Acinetobacter baylyi ADP1

52.252

96.69

0.505
  pilB Legionella pneumophila strain ERS1305867

50.635

95.993

0.486
  pilB Vibrio cholerae strain A1552

44.956

98.432

0.443
  pilB Vibrio campbellii strain DS40M4

45.756

94.425

0.432
  pilB Vibrio parahaemolyticus RIMD 2210633

45.522

93.38

0.425
  pilF Thermus thermophilus HB27

38.342

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.809

96.516

0.375

Multiple sequence alignment