Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABQY64_RS15800 Genome accession   NZ_CP168194
Coordinates   3777715..3779451 (-) Length   578 a.a.
NCBI ID   WP_349779527.1    Uniprot ID   -
Organism   Xanthomonas sp. WHRI 7065     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3772715..3784451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQY64_RS15785 (ABQY64_015785) sucC 3772883..3774052 (-) 1170 WP_039519379.1 ADP-forming succinate--CoA ligase subunit beta -
  ABQY64_RS15790 (ABQY64_015790) - 3774284..3775897 (+) 1614 WP_039512337.1 PAS domain-containing sensor histidine kinase -
  ABQY64_RS15795 (ABQY64_015795) pilR 3776090..3777484 (+) 1395 WP_016901261.1 sigma-54 dependent transcriptional regulator Regulator
  ABQY64_RS15800 (ABQY64_015800) pilB 3777715..3779451 (-) 1737 WP_349779527.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABQY64_RS15805 (ABQY64_015805) - 3779516..3779917 (-) 402 WP_221238164.1 pilin -
  ABQY64_RS15810 (ABQY64_015810) pilE 3780030..3780470 (-) 441 WP_039812285.1 pilin Machinery gene
  ABQY64_RS15815 (ABQY64_015815) pilC 3780794..3782050 (+) 1257 WP_039812282.1 type II secretion system F family protein Machinery gene
  ABQY64_RS15820 (ABQY64_015820) - 3782057..3782920 (+) 864 WP_016849610.1 A24 family peptidase -
  ABQY64_RS15825 (ABQY64_015825) coaE 3782934..3783572 (+) 639 WP_349779526.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62693.85 Da        Isoelectric Point: 5.4491

>NTDB_id=1042995 ABQY64_RS15800 WP_349779527.1 3777715..3779451(-) (pilB) [Xanthomonas sp. WHRI 7065]
MSVPLTANLVGITGIARRLVQDGALEEATARSAMDQAATAKIPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1042995 ABQY64_RS15800 WP_349779527.1 3777715..3779451(-) (pilB) [Xanthomonas sp. WHRI 7065]
ATGAGCGTTCCATTGACTGCAAATCTTGTTGGAATCACTGGGATTGCTCGGCGCTTGGTGCAGGATGGTGCGCTGGAAGA
AGCCACTGCTCGAAGTGCGATGGATCAGGCGGCTACTGCCAAAATTCCTCTGCCGCAATGGTTTGCCGAAAAGAAGCTAG
TCTCTGCTGCTCAACTCGCTGCTGCCAACGCAGTCGAGTTCGGCATGCCGTTGATGGACGTTTCGGTGTTCGACGCAAAT
CAGAACGCCGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTAAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGATCAGATCCGGCGCACGTTAGAGCAGTGGCAAGCCAGCAATGCTGCGCTTGGCTCGTCGCTAGGT
GACGACGACGAGGGGATGGGCGATCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAAGCACCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACTTTGCCGACGTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTTGACGGCAGCGCGGCCAAGCTGGGAATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCAATCCATAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCACTCGGCATCCTCAACGACGAGACGCGCAACATCTCCACGGCCGAGGACCCGGTGGAAATCCGTCTGCCCG
GCGTGAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGACGCGCCGCAGACCATCGCACGTTTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGAGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCGGAAGGGTTCACTGCAGAGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGTACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTC
AAGGCCGCGCACGGGGTGACTAGCCTGGCCGAGATCAATCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio cholerae strain A1552

49.458

95.848

0.474
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45

Multiple sequence alignment