Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABQ014_RS16800 Genome accession   NZ_CP168178
Coordinates   3945171..3946907 (-) Length   578 a.a.
NCBI ID   WP_104551201.1    Uniprot ID   A0A9X4H7Y5
Organism   Xanthomonas hortorum pv. hederae strain WHRI 7733A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3940171..3951907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ014_RS16780 (ABQ014_016780) - 3941125..3942738 (+) 1614 WP_104551197.1 PAS domain-containing sensor histidine kinase -
  ABQ014_RS16785 (ABQ014_016785) pilR 3942884..3944278 (+) 1395 WP_074058079.1 sigma-54 dependent transcriptional regulator Regulator
  ABQ014_RS16790 (ABQ014_016790) - 3944350..3944532 (+) 183 WP_104551199.1 YhfG family protein -
  ABQ014_RS16795 (ABQ014_016795) - 3944529..3945128 (+) 600 WP_104551200.1 Fic family protein -
  ABQ014_RS16800 (ABQ014_016800) pilB 3945171..3946907 (-) 1737 WP_104551201.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABQ014_RS16805 (ABQ014_016805) - 3946949..3947362 (-) 414 WP_104551202.1 pilin -
  ABQ014_RS16810 (ABQ014_016810) comP 3947459..3947887 (-) 429 WP_104551203.1 pilin Machinery gene
  ABQ014_RS16815 (ABQ014_016815) pilC 3948233..3949492 (+) 1260 WP_104551204.1 type II secretion system F family protein Machinery gene
  ABQ014_RS16820 (ABQ014_016820) - 3949499..3950362 (+) 864 WP_002812278.1 A24 family peptidase -
  ABQ014_RS16825 (ABQ014_016825) coaE 3950376..3950999 (+) 624 WP_104551205.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62854.07 Da        Isoelectric Point: 5.7680

>NTDB_id=1042923 ABQ014_RS16800 WP_104551201.1 3945171..3946907(-) (pilB) [Xanthomonas hortorum pv. hederae strain WHRI 7733A]
MNVAISSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLKEGFTEEQIAAGVELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1042923 ABQ014_RS16800 WP_104551201.1 3945171..3946907(-) (pilB) [Xanthomonas hortorum pv. hederae strain WHRI 7733A]
ATGAACGTTGCAATCAGCTCAAATCTAGTTGGGATTACCGGCATTGCGCGGCGCCTTGTTCAGGATGGCGCGCTCGATGA
GGCGAGTGCACGTGCGGCAATGGATCATGCAACCCAGGCCAAGGTTCCTTTGCCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCGCAGCTCGCGGCCGCCAACGCGGTCGAGTTCGGTATGCCGTTGATGGACGTGTCGGTCTTCGACGCCAAC
CAGAACGCAGTCAAGCTTGTCAGTGAAGAGTTACTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTAGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGT
GACGACGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCGTACGAAGACGACTACCGGGTGCGCTTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCG
GTAAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGTTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAACCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCG
GCGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTTGAGACGGCCGAGATCGCAATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGTATTGCGCCGTACAACATCA
CTTCGTCGGTGACCTTGGTTATTGCGCAGCGTTTGGCGCGGCGTTTGTGCAACAACTGCAAGCGTAAGGCACCGCTTCCG
GAGCACGCGCTATTGAAGGAAGGTTTTACCGAGGAGCAGATCGCTGCCGGTGTTGAACTTTACGAAGCAGTCGGTTGCGA
CGAATGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCGGCGCAGAGAATCGGCATCCGCGATCTACGCCAGTCGGCGCTGGTG
AAGGCGGCGCATGGGGTGACGAGCCTGGCCGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

51.211

100

0.512
  pilB Vibrio cholerae strain A1552

48.522

99.481

0.483
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

45.893

96.886

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

35.4

100

0.375
  pilF Thermus thermophilus HB27

40.883

90.138

0.369

Multiple sequence alignment