Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDH76_RS16535 Genome accession   NZ_CP168000
Coordinates   3781915..3783651 (-) Length   578 a.a.
NCBI ID   WP_277575597.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. cassiae strain NCPPB 645     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3776915..3788651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH76_RS16525 (ACDH76_16525) - 3777277..3778890 (+) 1614 WP_218490629.1 PAS domain-containing sensor histidine kinase -
  ACDH76_RS16530 (ACDH76_16530) pilR 3779157..3780611 (+) 1455 WP_372357657.1 sigma-54-dependent transcriptional regulator Regulator
  ACDH76_RS16535 (ACDH76_16535) pilB 3781915..3783651 (-) 1737 WP_277575597.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDH76_RS16540 (ACDH76_16540) - 3783748..3784395 (-) 648 WP_277573648.1 methionine biosynthesis protein MetW -
  ACDH76_RS16545 (ACDH76_16545) - 3784388..3785416 (-) 1029 WP_372162906.1 glycosyltransferase -
  ACDH76_RS16550 (ACDH76_16550) - 3785431..3786225 (-) 795 WP_372357658.1 glycosyltransferase -
  ACDH76_RS16555 (ACDH76_16555) - 3786230..3786580 (-) 351 WP_372162904.1 SMR family transporter -
  ACDH76_RS16560 (ACDH76_16560) - 3786957..3787373 (+) 417 WP_372357659.1 pilin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62614.66 Da        Isoelectric Point: 5.4503

>NTDB_id=1041073 ACDH76_RS16535 WP_277575597.1 3781915..3783651(-) (pilB) [Xanthomonas axonopodis pv. cassiae strain NCPPB 645]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAAAAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSSFDPN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1041073 ACDH76_RS16535 WP_277575597.1 3781915..3783651(-) (pilB) [Xanthomonas axonopodis pv. cassiae strain NCPPB 645]
ATGAATAGCGTCGTCACGGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
GGGCGCGGCGCGTGCTGCGATGGATCAGGCTGCTGCTGCAAAGGTCCCGTTGCCTCAATGGTTCTCGGAAAAAAAGTTGG
TGACAGCTTCGCAACTTGCGGCGGCCAACGCCGTGGAGTTTGGGATGCCGCTACTTGACGTGTCGTCGTTCGACCCTAAT
CAGAATGCAATTAAATTGGTTAGCGAAGAATTGCTGCAAAAGTTTCAAGTGCTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTAGGGGTGAGCAACCCGACCCAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTTGAGGCCA
TTCTTGTCGACGAGGATCAGATCCGTCGGACGCTCGAGCAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACACCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGACTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTATGGCATGGTATTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATCCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACGCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATCGCTGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGCGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilF Neisseria gonorrhoeae MS11

49.824

98.27

0.49
  pilB Vibrio cholerae strain A1552

50.181

95.848

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

97.578

0.45
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.617

100

0.386

Multiple sequence alignment