Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABRP31_RS23135 Genome accession   NZ_CP167995
Coordinates   5098344..5100038 (+) Length   564 a.a.
NCBI ID   WP_087274562.1    Uniprot ID   A0A1Y3NT77
Organism   Pseudomonas caspiana strain WHRI 7043     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5093344..5105038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP31_RS23115 (ABRP31_023115) - 5093846..5096095 (-) 2250 WP_349969606.1 DUF1631 domain-containing protein -
  ABRP31_RS23120 (ABRP31_023120) nadC 5096457..5097305 (+) 849 WP_087264928.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABRP31_RS23130 (ABRP31_023130) - 5097697..5098119 (-) 423 WP_349969605.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABRP31_RS23135 (ABRP31_023135) pilB 5098344..5100038 (+) 1695 WP_087274562.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABRP31_RS23140 (ABRP31_023140) pilC 5100041..5101258 (+) 1218 WP_087274561.1 type II secretion system F family protein Machinery gene
  ABRP31_RS23145 (ABRP31_023145) pilD 5101261..5102133 (+) 873 WP_349969604.1 A24 family peptidase Machinery gene
  ABRP31_RS23150 (ABRP31_023150) coaE 5102130..5102753 (+) 624 WP_349969603.1 dephospho-CoA kinase -
  ABRP31_RS23155 (ABRP31_023155) yacG 5102750..5102950 (+) 201 WP_087274556.1 DNA gyrase inhibitor YacG -
  ABRP31_RS23160 (ABRP31_023160) - 5103078..5103764 (-) 687 WP_349969602.1 energy-coupling factor ABC transporter permease -
  ABRP31_RS23165 (ABRP31_023165) - 5103761..5104228 (-) 468 WP_349969601.1 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62316.78 Da        Isoelectric Point: 6.5124

>NTDB_id=1040988 ABRP31_RS23135 WP_087274562.1 5098344..5100038(+) (pilB) [Pseudomonas caspiana strain WHRI 7043]
MSDVVLSGLAKQLVVAELLTEKAAQQAYQQARRDKVSLISYLVQNRLVRALVLAEMSSDHFGLPFLDLNCLNKEEQPKGL
VSEKLIRQHHALPLWKRANKLYIGISDPANQQAITDLQFSTGLAIEAILVEDDKLTDAIEKFFDGHSALGDMGDVDLSLE
VVSGSDTKETSLGQDDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKTFRVRLRTDGILHEVAKPPIHLASRIAARLKV
MASLDISERRKPQDGRVKLRISKTKAIDFRMNTLPTLWGEKIVMRLLDPTTAKMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPHCKKEADIPRETLIKEGFPEDK
VGTFKIYTAVGCDQCNGGYKGRVGIYEVVKKTPALERIIMEEGNSLDISAQMRKDGFNDLRTSGLIKAMQGVTSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1040988 ABRP31_RS23135 WP_087274562.1 5098344..5100038(+) (pilB) [Pseudomonas caspiana strain WHRI 7043]
ATGAGTGATGTTGTTCTGAGCGGTTTGGCCAAACAATTGGTAGTTGCCGAACTTCTGACTGAAAAAGCGGCGCAACAGGC
TTATCAGCAAGCCCGGCGCGACAAGGTTTCGCTGATCAGTTATCTGGTGCAAAACCGCTTGGTCAGGGCGCTGGTTCTGG
CCGAAATGTCCTCGGACCATTTCGGCCTGCCCTTTCTCGACCTCAATTGCCTTAATAAAGAAGAGCAGCCCAAGGGTTTG
GTGAGTGAAAAACTCATCCGCCAGCATCATGCCCTGCCGCTCTGGAAACGTGCCAACAAGCTCTATATCGGCATCTCCGA
CCCAGCCAATCAACAGGCCATAACCGATCTGCAGTTCAGCACCGGGCTGGCTATCGAAGCGATTCTGGTGGAGGACGACA
AGCTCACCGACGCCATCGAAAAATTCTTCGACGGCCACTCGGCTCTGGGCGACATGGGTGATGTCGATCTCAGCCTGGAA
GTGGTGTCGGGCAGCGACACCAAGGAAACCTCTCTCGGTCAGGACGATGCCGACGACGCGCCGGTGGTGCGTTTCGTCAA
CAAAATGCTGATGGACGCCATTCGCCTGGGCTCCTCCGACTTGCACTTCGAGCCTTACGAAAAAACCTTCCGGGTCCGGC
TACGCACCGACGGTATCCTGCATGAAGTGGCCAAACCGCCGATCCACCTCGCCAGCCGCATCGCAGCGCGTTTGAAGGTC
ATGGCCAGCCTGGATATTTCCGAGCGCCGCAAGCCGCAGGATGGCCGGGTCAAATTGCGCATTTCGAAAACCAAAGCCAT
TGATTTCCGGATGAACACCCTGCCGACGCTGTGGGGCGAAAAGATCGTGATGCGACTGCTCGACCCGACCACGGCGAAAA
TGGGCATCGACGCACTGGGCTATGAACCGGAGCAGAAAGCCCTGTATCTGGAGGCGCTCAAGCAGCCGCAAGGCATGATT
CTGGTCACCGGCCCTACTGGTTCGGGTAAGACTGTGTCGCTGTACACCGGCTTGAACATCCTCAACACCGTGGACATCAA
CATTTCAACCGCTGAAGATCCGGTCGAGATCAACCTTGAAGGCGTTAACCAGGTCAACGTCAATCCGCGTCAAGGAATGG
ACTTCTCCCAGGCACTTCGTGCGTTCCTGCGTCAGGATCCTGACGTAATCATGGTCGGTGAGATTCGTGACCTGGAAACC
GCCGAAATTGCGATCAAGGCTTCGCAGACCGGGCATATGGTGCTTTCCACCCTGCACACCAACAGCGCAGCAGAAACCCT
GACCCGCCTGCATCACATGGGCGTGGCCGCCTTCAATATTGCCACGGCCATCAACCTGATCATTGCCCAGCGTCTGGCGC
GCAAACTCTGCCCGCACTGCAAGAAGGAAGCAGACATACCCCGCGAAACCCTGATCAAGGAAGGCTTCCCGGAAGACAAA
GTCGGCACCTTCAAAATCTACACTGCCGTAGGCTGCGATCAGTGCAACGGCGGTTACAAGGGCCGCGTCGGGATTTACGA
GGTGGTCAAGAAGACCCCGGCTCTGGAGCGCATCATCATGGAAGAAGGCAACTCTCTGGATATCTCCGCTCAAATGCGCA
AGGACGGCTTTAATGACCTGCGCACTTCAGGCTTGATCAAAGCCATGCAGGGCGTCACCAGCCTGGAAGAAGTCAACCGC
GTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y3NT77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.94

99.645

0.567
  pilB Acinetobacter baumannii D1279779

56.762

99.645

0.566
  pilB Legionella pneumophila strain ERS1305867

54.064

100

0.543
  pilB Vibrio cholerae strain A1552

50.446

99.468

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.639

98.227

0.488
  pilB Vibrio campbellii strain DS40M4

48.404

100

0.484
  pilF Neisseria gonorrhoeae MS11

47.772

99.468

0.475
  pilF Thermus thermophilus HB27

37.128

100

0.376

Multiple sequence alignment