Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACCM60_RS27420 Genome accession   NZ_CP167916
Coordinates   6033237..6034454 (+) Length   405 a.a.
NCBI ID   WP_110177025.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aureofaciens strain W9-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6028237..6039454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCM60_RS27405 (ACCM60_27405) - 6028691..6031609 (+) 2919 WP_009050905.1 DUF748 domain-containing protein -
  ACCM60_RS27410 (ACCM60_27410) - 6031812..6032168 (-) 357 WP_009050906.1 BON domain-containing protein -
  ACCM60_RS27415 (ACCM60_27415) - 6032456..6032872 (-) 417 WP_009050907.1 pilin -
  ACCM60_RS27420 (ACCM60_27420) pilC 6033237..6034454 (+) 1218 WP_110177025.1 type II secretion system F family protein Machinery gene
  ACCM60_RS27425 (ACCM60_27425) pilD 6034457..6035329 (+) 873 WP_009050909.1 A24 family peptidase Machinery gene
  ACCM60_RS27430 (ACCM60_27430) coaE 6035326..6035949 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  ACCM60_RS27435 (ACCM60_27435) yacG 6035946..6036149 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  ACCM60_RS27440 (ACCM60_27440) - 6036154..6036369 (-) 216 WP_009050911.1 hypothetical protein -
  ACCM60_RS27445 (ACCM60_27445) - 6036435..6037124 (-) 690 WP_110177023.1 energy-coupling factor ABC transporter permease -
  ACCM60_RS27450 (ACCM60_27450) - 6037121..6037588 (-) 468 WP_009050913.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44361.10 Da        Isoelectric Point: 10.3398

>NTDB_id=1040206 ACCM60_RS27420 WP_110177025.1 6033237..6034454(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain W9-1]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVSKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGALLLSVFGLR
HAHRRSPRFRHWLDAALLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1040206 ACCM60_RS27420 WP_110177025.1 6033237..6034454(+) (pilC) [Pseudomonas chlororaphis subsp. aureofaciens strain W9-1]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCAGCCAGGGTCAGTAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCACTACGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAATTCGTGCAGCACTGGTGGTGGCTGCTGGCGGGCGCTCTGTTGCTCAGCGTGTTCGGCCTGCGT
CATGCCCACCGGCGCTCACCGCGCTTTCGCCACTGGCTGGACGCGGCCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTATTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCACTTTTCCATGCGCGCATCCGGCATTTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGACGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAAGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.679

100

0.657
  pilC Acinetobacter baylyi ADP1

55.637

100

0.56
  pilC Acinetobacter baumannii D1279779

54.902

100

0.553
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

42.289

99.259

0.42
  pilG Neisseria meningitidis 44/76-A

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393

Multiple sequence alignment