Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDW34_RS16345 Genome accession   NZ_CP167896
Coordinates   3349037..3350752 (+) Length   571 a.a.
NCBI ID   WP_286434881.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain p38     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3344037..3355752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW34_RS16305 (ACDW34_16305) nusA 3344596..3346080 (-) 1485 WP_130072255.1 transcription termination factor NusA -
  ACDW34_RS16310 (ACDW34_16310) rimP 3346121..3346645 (-) 525 WP_130072256.1 ribosome maturation factor RimP -
  ACDW34_RS16335 (ACDW34_16335) secG 3347456..3347788 (-) 333 WP_130072257.1 preprotein translocase subunit SecG -
  ACDW34_RS16340 (ACDW34_16340) tpiA 3347798..3348592 (-) 795 WP_372403656.1 triose-phosphate isomerase -
  ACDW34_RS16345 (ACDW34_16345) pilB 3349037..3350752 (+) 1716 WP_286434881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDW34_RS16350 (ACDW34_16350) pilC 3350779..3352005 (+) 1227 WP_130072260.1 type II secretion system F family protein Machinery gene
  ACDW34_RS16355 (ACDW34_16355) pilD 3352005..3352868 (+) 864 WP_130072261.1 A24 family peptidase Machinery gene
  ACDW34_RS16360 (ACDW34_16360) coaE 3352880..3353476 (+) 597 WP_174493876.1 dephospho-CoA kinase -
  ACDW34_RS16365 (ACDW34_16365) - 3353545..3354462 (-) 918 WP_372403657.1 DMT family transporter -
  ACDW34_RS16370 (ACDW34_16370) rlmB 3354506..3355258 (-) 753 WP_372403659.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACDW34_RS16375 (ACDW34_16375) - 3355398..3355721 (-) 324 WP_065993789.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63411.82 Da        Isoelectric Point: 4.9501

>NTDB_id=1040181 ACDW34_RS16345 WP_286434881.1 3349037..3350752(+) (pilB) [Acinetobacter piscicola strain p38]
MVSQQGTPKFSGFIRRLVDDGVIAAQQMQSALDNAKKSKLNIVAYLIEYANLKPLQIAETIALEFAEPLFDLDSFDPEQF
PKDVIESGLLHKHLMLPLVQRGNVLYIAMSDPTNIEALDAARFNSRLNIEVVIVEHHKLVKCLEKYFTEDTSFDFGGDDI
DVDIISTDDVNPADDHDDNQSEDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRSITNPPLQLATRLA
SRLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDK
PQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSVALKSFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTHCKAPVEIPKHSLLEMG
FTEEDLAHPDFQVFQPVGCPECREGYKGRVGVYEVMKVTPEISKIIMEDGNALEIAEASAKLGFNNLRRSGLIKVMNGLT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1040181 ACDW34_RS16345 WP_286434881.1 3349037..3350752(+) (pilB) [Acinetobacter piscicola strain p38]
ATGGTGTCACAACAAGGCACTCCGAAGTTTTCGGGGTTTATTCGTCGCTTAGTCGATGACGGTGTTATTGCTGCGCAACA
AATGCAATCTGCGCTAGACAATGCTAAAAAAAGTAAGCTCAACATCGTAGCTTATCTGATTGAATATGCAAATTTAAAAC
CTTTGCAAATTGCTGAAACCATTGCGCTTGAGTTCGCCGAGCCTTTGTTTGACCTTGATAGCTTTGATCCCGAGCAATTC
CCTAAAGATGTTATTGAAAGTGGTTTATTACATAAACATCTGATGCTGCCTTTGGTTCAACGTGGAAATGTGCTTTATAT
CGCCATGAGCGATCCAACCAATATTGAAGCTTTGGATGCTGCACGTTTTAATAGTCGCTTGAATATTGAAGTCGTCATCG
TTGAACATCACAAACTCGTCAAATGCTTAGAAAAATACTTTACTGAAGACACCAGTTTTGATTTTGGTGGTGATGATATT
GATGTTGATATCATCTCAACTGATGATGTAAACCCTGCAGATGATCACGACGATAATCAAAGCGAAGATGAAGCTCCAAT
TGTAAAGTATATTAATAAGTTACTTGTTGATGCTATTCGTATGGGTGCATCAGATTTACATTTTGAACCTTATGAAAAAT
CCTATCGTGTGCGTTATCGTGTGGATGGTGTGCTTAGAAGTATTACCAATCCCCCACTGCAACTTGCCACACGACTCGCA
TCACGTTTAAAAGTGATGTCACAAATGGATATTTCTGAAAAGCGTATTCCACAAGATGGTCGTATTAAACTTAAATTATC
CAAAACCAAAGCCATCGACTTTCGTGTCAACTCTCTCCCGACGCTATTTGGTGAAAAACTCGTACTGCGTATTTTAGATC
CATCCAGTGCGATGCTGGGTATTGATGCCTTAGGTTATGAGGAAGATCAAAAAGCTTTATTCATGGAAGCTTTAGATAAA
CCCCAAGGTATGCTCTTAATCACAGGACCGACAGGCTCTGGTAAAACGGTATCACTCTATACGGGTTTAAATATTTTAAA
TACTGAAAGTTCGAATATTTCAACAGCCGAAGACCCTGTAGAGATTAACTTAGAAGGGATTAATCAGGTCAATGTTAATC
CTAAAGTCGGACTGACGTTTTCTGTCGCCTTGAAATCTTTCTTACGTCAAGACCCTGATATCATTATGGTCGGGGAGATT
CGAGACCTTGAAACTGCTGAAATTGCGATTAAAGCAGCTCAAACAGGTCATATGGTCATGTCTACACTACATACCAATAG
TGCGCCTGAAACCTTAACACGTTTAAGAAATATGGGCGTTCCTTCTTTTAATATTGCAACCTCTGTCAATCTCGTCATTG
CACAACGTTTGGCACGCCGCTTATGTACACATTGTAAAGCACCTGTTGAAATTCCTAAACATAGCCTCTTAGAAATGGGC
TTTACTGAAGAGGATTTAGCACACCCTGATTTCCAAGTGTTTCAACCTGTGGGTTGCCCAGAATGTCGTGAAGGCTATAA
AGGGCGTGTCGGGGTATATGAAGTGATGAAAGTGACACCTGAGATTTCAAAAATTATTATGGAAGATGGTAATGCCTTAG
AAATTGCTGAAGCATCTGCCAAACTGGGGTTTAATAATTTACGACGCTCAGGACTAATTAAAGTCATGAATGGTTTAACA
TCACTACAAGAAATTAATCGCGTCACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-148)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.86

100

0.799
  pilB Acinetobacter baylyi ADP1

76.596

98.774

0.757
  pilF Neisseria gonorrhoeae MS11

49.824

99.475

0.496
  pilB Legionella pneumophila strain ERS1305867

51.654

95.271

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

48.122

97.898

0.471
  pilB Vibrio cholerae strain A1552

47.321

98.074

0.464
  pilB Vibrio campbellii strain DS40M4

47.217

97.548

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

35.294

100

0.378

Multiple sequence alignment