Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDH70_RS18190 Genome accession   NZ_CP167865
Coordinates   4270052..4271788 (+) Length   578 a.a.
NCBI ID   WP_228962907.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4265052..4276788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH70_RS18175 (ACDH70_18175) - 4266878..4267330 (+) 453 WP_228963894.1 GtrA family protein -
  ACDH70_RS18180 (ACDH70_18180) - 4267323..4268867 (+) 1545 WP_228963893.1 NAD(P)/FAD-dependent oxidoreductase -
  ACDH70_RS18185 (ACDH70_18185) galE 4268958..4269935 (+) 978 WP_372360353.1 UDP-glucose 4-epimerase GalE -
  ACDH70_RS18190 (ACDH70_18190) pilB 4270052..4271788 (+) 1737 WP_228962907.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDH70_RS18195 (ACDH70_18195) pilB 4271854..4271973 (+) 120 WP_029220459.1 hypothetical protein Machinery gene
  ACDH70_RS18200 (ACDH70_18200) pilB 4272039..4272158 (+) 120 WP_029220459.1 hypothetical protein Machinery gene
  ACDH70_RS18205 (ACDH70_18205) pilR 4272797..4274191 (-) 1395 WP_228962908.1 sigma-54 dependent transcriptional regulator Regulator
  ACDH70_RS18210 (ACDH70_18210) - 4274516..4276129 (-) 1614 WP_372360354.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62560.71 Da        Isoelectric Point: 5.7597

>NTDB_id=1040008 ACDH70_RS18190 WP_228962907.1 4270052..4271788(+) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939]
MNAVSTANLVGITGLARRLVQDGALDEAVARDAMDKAAAAKLPLPIWLAQNRVVGAAQLAAANAVEFGMPLLDVSAFDAS
QSAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGAALG
NDDDEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSTLP
DNALLAEGFTPEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1040008 ACDH70_RS18190 WP_228962907.1 4270052..4271788(+) (pilB) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939]
ATGAACGCCGTCAGTACCGCCAACCTTGTCGGAATCACTGGCCTAGCGCGCCGCTTGGTCCAGGATGGAGCGCTAGACGA
AGCCGTCGCGCGCGATGCGATGGATAAAGCAGCTGCAGCAAAGCTGCCGCTCCCAATCTGGCTTGCCCAGAACAGAGTTG
TGGGCGCGGCACAGCTAGCCGCAGCCAACGCTGTTGAGTTCGGTATGCCCTTGCTGGACGTATCAGCATTCGACGCCAGC
CAGAGCGCTGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTAAGCAACCCGACCCAGACCCGTGCGCTGGATGACATCAAATTTCATACCAACCTGGTGGTTGAGCCCA
TTCTTGTTGATGAGGATCAGATTCGCCGGACGTTGGAGCAGTGGCAGGCTGGTAATGCCTCGTTTGGAGCTGCGCTTGGG
AACGACGATGACGAGATGGGCGACTTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGGGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGGCTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAATCAGCGCATCGCGGCACGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGGCGGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCTTACGGGATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTATCGTT
GTACACGGCGCTGGGTATCCTCAACGACGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCTGCCTTGCGTTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCACAGACCGGTCACATGGT
GCTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAATATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTAACCCTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAGTCGACGCTGCCG
GATAATGCGTTGCTGGCCGAGGGTTTTACGCCTGAGCAGATCGCCGCGGGAATCGAGCTGTACGAGGCGGTGGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTATCAGGTAATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCGGAAGCGGCGCAGAAGATCGGCATCCGCGATTTGCGTCAGTCGGCGTTGATG
AAGGCTGCGCATGGGGTGACCAGTCTGGCCGAGATCAATCGGGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-150)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.786

100

0.559
  pilB Acinetobacter baylyi ADP1

55.613

100

0.557
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio cholerae strain A1552

50.638

94.983

0.481
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.936

97.924

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.439

92.561

0.365
  pilF Thermus thermophilus HB27

39.36

91.869

0.362

Multiple sequence alignment