Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ16_RS15665 Genome accession   NZ_CP167864
Coordinates   3625647..3627383 (-) Length   578 a.a.
NCBI ID   WP_277572535.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 1062     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620647..3632383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ16_RS15650 (ACCQ16_15650) sucC 3620696..3621865 (-) 1170 WP_057681815.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCQ16_RS15655 (ACCQ16_15655) - 3622097..3623710 (+) 1614 WP_218490629.1 PAS domain-containing sensor histidine kinase -
  ACCQ16_RS15660 (ACCQ16_15660) pilR 3624038..3625432 (+) 1395 WP_042773941.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ16_RS15665 (ACCQ16_15665) pilB 3625647..3627383 (-) 1737 WP_277572535.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ16_RS15670 (ACCQ16_15670) - 3627426..3627836 (-) 411 WP_029820067.1 pilin -
  ACCQ16_RS15675 (ACCQ16_15675) pilA 3628021..3628431 (-) 411 WP_029820068.1 pilin Machinery gene
  ACCQ16_RS15680 (ACCQ16_15680) pilC 3628765..3630021 (+) 1257 WP_014090856.1 type II secretion system F family protein Machinery gene
  ACCQ16_RS15685 (ACCQ16_15685) - 3630028..3630891 (+) 864 WP_008576868.1 A24 family peptidase -
  ACCQ16_RS15690 (ACCQ16_15690) coaE 3630905..3631510 (+) 606 WP_014090857.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62572.65 Da        Isoelectric Point: 5.7615

>NTDB_id=1039991 ACCQ16_RS15665 WP_277572535.1 3625647..3627383(-) (pilB) [Xanthomonas sp. NCPPB 1062]
MNAVSSANLVGITGIARRLVQDGAIEEPAARAAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSTLP
ENALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039991 ACCQ16_RS15665 WP_277572535.1 3625647..3627383(-) (pilB) [Xanthomonas sp. NCPPB 1062]
ATGAATGCTGTTTCGTCCGCTAATTTGGTTGGCATCACGGGAATTGCCCGTCGTTTAGTACAAGATGGCGCGATTGAAGA
GCCTGCTGCTCGGGCTGCGATGGATCAGGCAGCGGCTGCGAAGGTTCCATTGCCCCAGTGGTTTTCGGAAAAAAAGCTGG
TATCGGCGTCGCAACTGGCTGCTGCCAATGCAGTCGAGTTCGGTATGCCCCTGATGGATGTGTCGGCATTCGACGCAAGC
CAGAACGCAGTAAAGCTCGTCAGCGAAGAGTTGCTCCAGAAGCACCAAGTGTTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTGGGGGTGAGCAACCCAACCCAGACTCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTTGTCGACGAGGATCAGATCCGCCGCACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTTGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCTTGCCAG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTTTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CTTCGTCGGTGACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAGTCGACGCTGCCG
GAGAATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCCGCCGGGATCGAGCTGTACGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTTACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

98.27

0.554
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilB Vibrio cholerae strain A1552

49.74

99.827

0.497
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

96.886

0.452
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45

Multiple sequence alignment