Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ12_RS14940 Genome accession   NZ_CP167863
Coordinates   3545103..3546839 (-) Length   578 a.a.
NCBI ID   WP_372382091.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 1068     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3540103..3551839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ12_RS14925 (ACCQ12_14925) - 3540752..3542365 (+) 1614 WP_047695066.1 PAS domain-containing sensor histidine kinase -
  ACCQ12_RS14930 (ACCQ12_14930) pilR 3542916..3544310 (+) 1395 WP_372382090.1 sigma-54-dependent transcriptional regulator Regulator
  ACCQ12_RS14935 (ACCQ12_14935) pilB 3544735..3544854 (-) 120 WP_104579922.1 pilus assembly protein Machinery gene
  ACCQ12_RS14940 (ACCQ12_14940) pilB 3545103..3546839 (-) 1737 WP_372382091.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ12_RS14945 (ACCQ12_14945) galE 3546952..3547929 (-) 978 WP_372382092.1 UDP-glucose 4-epimerase GalE -
  ACCQ12_RS14950 (ACCQ12_14950) - 3548539..3549033 (+) 495 WP_372382093.1 class I SAM-dependent methyltransferase -
  ACCQ12_RS14955 (ACCQ12_14955) - 3549037..3549414 (-) 378 WP_372382094.1 EamA family transporter -
  ACCQ12_RS14960 (ACCQ12_14960) - 3549411..3550202 (-) 792 WP_372382095.1 glycosyltransferase family 2 protein -
  ACCQ12_RS14965 (ACCQ12_14965) - 3550204..3551394 (-) 1191 WP_372382096.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62570.80 Da        Isoelectric Point: 5.6039

>NTDB_id=1039973 ACCQ12_RS14940 WP_372382091.1 3545103..3546839(-) (pilB) [Xanthomonas sp. NCPPB 1068]
MNVVSTANLVGITGLARRLVQDGALDEAVARDAMEKAAAAKLPLPIWLAQNKVVGAAQLAAANAVEFGMPLLDVSAFDAS
QSALKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
SDDDDMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSSLP
ENALLAEGFTPEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAIQIAEAAQKIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039973 ACCQ12_RS14940 WP_372382091.1 3545103..3546839(-) (pilB) [Xanthomonas sp. NCPPB 1068]
ATGAACGTTGTCAGTACCGCTAACCTTGTCGGAATCACTGGCCTAGCGCGCCGCTTGGTCCAAGACGGAGCGCTGGATGA
AGCCGTTGCTCGCGATGCAATGGAGAAAGCAGCTGCAGCCAAGCTGCCACTCCCAATATGGCTTGCCCAGAACAAGGTCG
TAGGCGCGGCACAGCTGGCCGCCGCGAATGCGGTTGAGTTTGGCATGCCCTTGCTGGACGTATCAGCGTTCGACGCCAGC
CAAAGTGCCCTCAAGCTGGTTAGTGAAGAGTTGCTCCAAAAGTACCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACTCAGACCCGGGCGCTGGATGACATCAAGTTTCATACAAACCTGGTAGTTGAGGCGA
TTCTTGTTGACGAGGATCAGATCCGCCGGACACTTGAGCAATGGCAGGCCAGCAATGCGTCGCTTGGTTCGTCGCTGGGA
AGCGACGACGACGACATGGGTGATCTGGATGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGGGACGACACGCCGGTGGTGAAGTTCGTTAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCCGACA
TCCATTTCGAACCTTATGAAGACGACTATCGGGTGCGCTTGCGCATCGACGGACTGCTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAACGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGTACCTTGCCGACCTTGTTTGGTGAGA
AGGTGGTGCTTCGTATCCTGGATGCCAGTGCGGCCAAGTTGGGTATCGAGAAGCTGGGCTACGAGGCGGATCAGCAAAAG
CTGTTCCTGGAAGCGATCCACAAGCCTTATGGCATGGTGCTGGTGACCGGACCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAATGATGAGACCCGTAATATTTCCACGGCTGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAACGCGGCATGACGTTTGCTGCGGCGCTGCGCTCATTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACTGGTCACATGGT
GCTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACCATTGCAAGCGCAAGTCGTCGCTGCCT
GAAAATGCGTTGCTTGCTGAAGGCTTTACGCCAGAGCAGATCGCTGCCGGGATCGAACTGTATGAGGCGGTGGGCTGCGA
CGAATGCACCGAAGGCTACAAGGGGCGCACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATTGGCGCCATTGTGC
TGGAAGGCGGCAATGCAATACAGATCGCCGAGGCCGCGCAGAAGATTGGTATCCGCGATCTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCTTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Acinetobacter baumannii D1279779

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

49.726

94.637

0.471
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.936

97.924

0.45
  pilF Thermus thermophilus HB27

39.548

91.869

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.106

92.907

0.363

Multiple sequence alignment