Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ04_RS15560 Genome accession   NZ_CP167851
Coordinates   3657469..3659205 (-) Length   578 a.a.
NCBI ID   WP_226427346.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 2866     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3652469..3664205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ04_RS15545 (ACCQ04_15545) sucC 3652654..3653823 (-) 1170 WP_039519379.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCQ04_RS15550 (ACCQ04_15550) - 3654055..3655668 (+) 1614 WP_016901263.1 PAS domain-containing sensor histidine kinase -
  ACCQ04_RS15555 (ACCQ04_15555) pilR 3655861..3657255 (+) 1395 WP_051634076.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ04_RS15560 (ACCQ04_15560) pilB 3657469..3659205 (-) 1737 WP_226427346.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ04_RS15565 (ACCQ04_15565) - 3659461..3659895 (-) 435 WP_104646205.1 pilin -
  ACCQ04_RS15570 (ACCQ04_15570) pilC 3660247..3661503 (+) 1257 WP_226427347.1 type II secretion system F family protein Machinery gene
  ACCQ04_RS15575 (ACCQ04_15575) - 3661510..3662373 (+) 864 WP_016849610.1 A24 family peptidase -
  ACCQ04_RS15580 (ACCQ04_15580) coaE 3662387..3663010 (+) 624 WP_054592195.1 dephospho-CoA kinase -
  ACCQ04_RS15585 (ACCQ04_15585) - 3663020..3663265 (+) 246 WP_372358788.1 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62674.89 Da        Isoelectric Point: 5.6006

>NTDB_id=1039850 ACCQ04_RS15560 WP_226427346.1 3657469..3659205(-) (pilB) [Xanthomonas sp. NCPPB 2866]
MNVAATVNLVGITGIARRLVQDGVLDEGIARAAMDQAASAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGVGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039850 ACCQ04_RS15560 WP_226427346.1 3657469..3659205(-) (pilB) [Xanthomonas sp. NCPPB 2866]
ATGAACGTAGCCGCCACTGTTAATCTAGTCGGGATCACCGGAATTGCTCGTCGTCTGGTTCAGGACGGTGTATTGGACGA
AGGTATCGCACGGGCCGCAATGGATCAGGCCGCATCGGCCAAGGTCCCGTTACCGCAGTGGTTCGCCGAAAAGAAGCTGG
TCACTGCCTCTCAACTTGCAGCAGCAAACGCTGTCGAATTCGGCATGCCCTTGATGGACGTGTCGGTCTTCGACGCCAAC
CAGAACGCAGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATAAAGTTCCATACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGT
GACGACGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGTTGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCCTATGAGGACGACTACCGGGTGCGCTTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCG
GTAAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAACCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCACTCGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCAGTAGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTTCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGACGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATTGCCCCTTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCTATGCAGATCGCCGAGGCGGCACAGAGTATCGGCATCCGTGACCTGCGCCAGTCGGCGTTGGTC
AAGGCCGCGCACGGTGTGACCAGCCTGGCCGAGATCAACCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.27

0.547
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilB Vibrio cholerae strain A1552

49.819

95.848

0.478
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

46.071

96.886

0.446
  pilF Thermus thermophilus HB27

40.192

89.965

0.362

Multiple sequence alignment