Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCQ03_RS16340 Genome accession   NZ_CP167847
Coordinates   3771300..3773009 (-) Length   569 a.a.
NCBI ID   WP_057677884.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 3569     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3766300..3778009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ03_RS16320 (ACCQ03_16320) - 3767378..3768991 (+) 1614 WP_209225620.1 PAS domain-containing sensor histidine kinase -
  ACCQ03_RS16325 (ACCQ03_16325) pilR 3769253..3770707 (+) 1455 WP_017155148.1 sigma-54 dependent transcriptional regulator Regulator
  ACCQ03_RS16330 (ACCQ03_16330) - 3770911..3771042 (-) 132 Protein_3195 serine/threonine protein kinase -
  ACCQ03_RS16335 (ACCQ03_16335) - 3771119..3771238 (-) 120 WP_057687106.1 hypothetical protein -
  ACCQ03_RS16340 (ACCQ03_16340) pilB 3771300..3773009 (-) 1710 WP_057677884.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCQ03_RS16345 (ACCQ03_16345) pilA 3773167..3773595 (-) 429 WP_057677883.1 pilin Machinery gene
  ACCQ03_RS16350 (ACCQ03_16350) pilC 3773936..3775198 (+) 1263 WP_057677885.1 type II secretion system F family protein Machinery gene
  ACCQ03_RS16355 (ACCQ03_16355) - 3775205..3776068 (+) 864 WP_003491180.1 A24 family peptidase -
  ACCQ03_RS16360 (ACCQ03_16360) coaE 3776082..3776690 (+) 609 WP_022558665.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61685.58 Da        Isoelectric Point: 5.4503

>NTDB_id=1039816 ACCQ03_RS16340 WP_057677884.1 3771300..3773009(-) (pilB) [Xanthomonas sp. NCPPB 3569]
MGITGIARRLVQDGALDEASARTAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDANQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1039816 ACCQ03_RS16340 WP_057677884.1 3771300..3773009(-) (pilB) [Xanthomonas sp. NCPPB 3569]
GTGGGAATTACAGGCATTGCACGCCGTTTGGTGCAGGATGGAGCACTGGATGAGGCAAGTGCCCGTACCGCAATGGACCA
AGCCGCGGCGGCGAAGATTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTTCAGCGTCTCAATTGGCCGCTGCCA
ATGCAGTCGAGTTCGGCATGCCACTACTGGACGTGTCGGCATTCGACGCCAACCAAAACGCGGTGAAGCTGGTCAGCGAG
GAATTGCTCCAGAAGCATCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAGATGGGGGATCTG
GACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATTAGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCGGTGAAGCTGAATCAGCGCATCGCAGCA
CGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAAGCGATCCACAAGCCG
TACGGGATGGTACTGGTGACCGGGCCGACTGGCTCGGGCAAGACCGTGTCGCTGTACACGGCGCTGGGTATCCTCAATGA
CGAGACGCGCAATATCTCCACTGCGGAGGACCCGGTCGAAATCCGCTTGCCTGGTGTCAACCAGGTGCAGCAGAACAACA
AGCGTGGCATGACCTTCGCCGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACCGCCGAAATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCACAGACCATTGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCCTGCCTGATAACGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCAGGGATAGAGCTGTATGAGGCGGTTGGCTGCGATGAGTGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAAGCCGCGCAGGCGATCGGTATCCGCGATTTGCGTCAGTCGGCGCTGATGAAGGCTGCGAATGGGGTGACCAGCCTG
GCAGAGATCAACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

99.824

0.566
  pilB Acinetobacter baylyi ADP1

55.458

99.824

0.554
  pilB Legionella pneumophila strain ERS1305867

53.076

100

0.531
  pilB Vibrio cholerae strain A1552

48.866

100

0.492
  pilF Neisseria gonorrhoeae MS11

49.296

99.824

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.97

0.366
  pilF Thermus thermophilus HB27

39.423

91.388

0.36

Multiple sequence alignment