Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABQ179_RS05735 Genome accession   NZ_CP167817
Coordinates   1343349..1345070 (+) Length   573 a.a.
NCBI ID   WP_104616299.1    Uniprot ID   A0A2S7C0N7
Organism   Xanthomonas dyei strain WHRI 5755     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1338349..1350070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ179_RS05715 (ABQ179_005715) coaE 1339534..1340139 (-) 606 WP_104616295.1 dephospho-CoA kinase -
  ABQ179_RS05720 (ABQ179_005720) - 1340153..1341016 (-) 864 WP_055848873.1 A24 family peptidase -
  ABQ179_RS05725 (ABQ179_005725) pilC 1341023..1342282 (-) 1260 WP_311238931.1 type II secretion system F family protein Machinery gene
  ABQ179_RS05730 (ABQ179_005730) - 1342625..1343032 (+) 408 WP_349811581.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABQ179_RS05735 (ABQ179_005735) pilB 1343349..1345070 (+) 1722 WP_104616299.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABQ179_RS05740 (ABQ179_005740) pilR 1345376..1346770 (-) 1395 WP_170068658.1 sigma-54 dependent transcriptional regulator Regulator
  ABQ179_RS05750 (ABQ179_005750) - 1347268..1348884 (-) 1617 WP_104616301.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62583.65 Da        Isoelectric Point: 5.6021

>NTDB_id=1039547 ABQ179_RS05735 WP_104616299.1 1343349..1345070(+) (pilB) [Xanthomonas dyei strain WHRI 5755]
MNQNLVGITGIARRLVQDGVIDESVARSAMEQASTAKIPLPQWFSDKKLVSAPQLAAANAVEFGMPLMDVSVFDANQNAV
KLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLGNDDD
DIGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLS
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHAL
LAEGFTPEQIANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRVGIRDLRQSALMKASHG
VTSLAEINRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1039547 ABQ179_RS05735 WP_104616299.1 1343349..1345070(+) (pilB) [Xanthomonas dyei strain WHRI 5755]
ATGAATCAGAATTTGGTTGGCATCACCGGTATCGCGCGGCGTCTAGTTCAAGATGGCGTGATAGACGAGTCTGTTGCCCG
CTCAGCAATGGAGCAGGCGTCAACGGCAAAGATTCCTCTGCCGCAGTGGTTTTCCGATAAGAAACTAGTTTCAGCGCCTC
AACTTGCCGCTGCCAACGCAGTCGAGTTCGGCATGCCCTTGATGGACGTGTCTGTTTTCGATGCCAACCAGAACGCGGTC
AAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTATTCGTGGGGGT
GAGCAACCCAACCCAGACCCGGGCGCTGGACGACATCAAGTTTCACACCAATCTGGTGGTGGAGCCGATCCTGGTCGATG
AGGATCAGATCCGCCGGACGCTTGAGCAGTGGCAGGCAAGCAATGCGTCGCTAGGTTCATCGCTCGGCAATGACGATGAC
GACATCGGTGACCTGGACGTGTCGTCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGTGA
CGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCCGACATCCATTTCGAGC
CGTATGAAGATGACTATCGGGTGCGCTTGCGCATCGATGGCTTGCTGAAGAACGTGGCCAAGGCGCCGGTGAAGTTGAGT
CAGCGCATTGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCGGAGAAGCGGGTGCCGCAGGACGGGCGAATCAA
GCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGTACCTTGCCGACGCTGTTCGGCGAGAAGGTGGTGCTGC
GTATTTTGGACGCCAGTGCAGCCAAGCTGGGTATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGAT
GCGATCCACAAGCCATACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTTGTATACGGCGCT
AGGCATCCTCAACGACGAGACACGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCGGCGTGAATCAGG
TACAGCAGAACGTCAAGCGCGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTTGAGACAGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCATATGGTGCTGTCGACGCT
GCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAATATGGGCATTGCGCCTTACAACATCACCTCGTCGGTCA
CGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAGCATGCGCTG
CTGGCGGAAGGCTTCACGCCCGAGCAGATCGCGAACATCGAGCTGTATGAGGCGGTGGGCTGCGATGAGTGTACCGAGGG
GTACAAGGGTCGTACCGGTATCTATCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGCAATG
CGATGCAGATTGCCGAGGCCGCACAGAGGGTAGGTATCCGCGATCTGCGCCAGTCGGCGTTGATGAAGGCTTCGCATGGG
GTAACCAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-149)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7C0N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.986

99.127

0.555
  pilB Acinetobacter baumannii D1279779

54.93

99.127

0.545
  pilB Legionella pneumophila strain ERS1305867

52.289

99.127

0.518
  pilB Vibrio cholerae strain A1552

48.7

100

0.49
  pilF Neisseria gonorrhoeae MS11

48.768

99.127

0.483
  pilB Vibrio campbellii strain DS40M4

46.397

99.302

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

45.581

100

0.459
  pilF Thermus thermophilus HB27

40.077

90.576

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.057

92.496

0.361

Multiple sequence alignment