Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDJ02_RS15075 Genome accession   NZ_CP167777
Coordinates   3184801..3186432 (+) Length   543 a.a.
NCBI ID   WP_372376521.1    Uniprot ID   -
Organism   Acinetobacter ursingii strain E5131942     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179801..3191432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDJ02_RS15040 (ACDJ02_15040) nusA 3180492..3181976 (-) 1485 WP_004994250.1 transcription termination factor NusA -
  ACDJ02_RS15045 (ACDJ02_15045) rimP 3182012..3182536 (-) 525 WP_151724394.1 ribosome maturation factor RimP -
  ACDJ02_RS15065 (ACDJ02_15065) secG 3183222..3183551 (-) 330 WP_125269307.1 preprotein translocase subunit SecG -
  ACDJ02_RS15070 (ACDJ02_15070) tpiA 3183561..3184358 (-) 798 WP_151724393.1 triose-phosphate isomerase -
  ACDJ02_RS15075 (ACDJ02_15075) pilB 3184801..3186432 (+) 1632 WP_372376521.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDJ02_RS15080 (ACDJ02_15080) pilC 3186463..3187689 (+) 1227 WP_004994255.1 type II secretion system F family protein Machinery gene
  ACDJ02_RS15085 (ACDJ02_15085) pilD 3187689..3188549 (+) 861 WP_151724660.1 A24 family peptidase Machinery gene
  ACDJ02_RS15090 (ACDJ02_15090) coaE 3188552..3189145 (+) 594 WP_151724661.1 dephospho-CoA kinase -
  ACDJ02_RS15095 (ACDJ02_15095) - 3189127..3190050 (-) 924 WP_151724662.1 DMT family transporter -
  ACDJ02_RS15100 (ACDJ02_15100) rlmB 3190091..3190840 (-) 750 WP_151724663.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACDJ02_RS15105 (ACDJ02_15105) - 3190960..3191286 (-) 327 WP_004991681.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 543 a.a.        Molecular weight: 60937.13 Da        Isoelectric Point: 5.5569

>NTDB_id=1039329 ACDJ02_RS15075 WP_372376521.1 3184801..3186432(+) (pilB) [Acinetobacter ursingii strain E5131942]
MFYEINKNNKFIVQYLVEAFDIKPSIIAEAISIEYEEPQFDLNTFNLSLIPHGIIDEKLITKHKILPLSVKDHTLYVAVV
DPSLTQDLDTIRFNSKKKLELVIVEHDKLERALEEVYGTNAQLEFLNSLHNEDSENTEDILSEFSFDINADHEKDDTIVV
KYLNKLLYDAIKMGASDLHFEPYAKIYRVRYRIDGVLHAISFPPLVLAPRLTVRLKIMAKMDISERRSPQDGRIKLPVSQ
FKSIDFRVSSLPTLYGEKLVLRILDASSATLGIDALGYEEDQKALFLEALNKPQGMLLITGPTGSGKTVSLYTGLNILNT
ESSNISTAEDPVEINLEGVNQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIRDLETAEIAIKAAQTGHMVMSTLHTNSA
PETLTRLRNMGVPSFNLATSVNLVIAQRLARRLCSKCKEPIQIPRNSLIEMGFTEEDLSHPKLRIYQPVGCSECREGYKG
RVGIYEVMKVTPEISRIIMEDGNAIQIADASEKLGFNNLRRSGLKKVMQGITSLQEVNRVTSE

Nucleotide


Download         Length: 1632 bp        

>NTDB_id=1039329 ACDJ02_RS15075 WP_372376521.1 3184801..3186432(+) (pilB) [Acinetobacter ursingii strain E5131942]
ATGTTTTATGAAATAAATAAAAATAACAAATTTATTGTTCAGTATCTTGTAGAGGCCTTTGATATAAAGCCATCAATTAT
TGCTGAAGCTATTTCTATTGAATATGAAGAACCCCAATTTGATCTTAATACATTTAATCTCTCTTTGATTCCTCATGGCA
TTATCGATGAAAAATTAATCACAAAGCATAAAATTTTACCTCTAAGCGTAAAAGACCACACCTTATATGTTGCTGTTGTA
GACCCCTCTCTTACTCAAGATTTAGATACAATTCGCTTTAATAGTAAGAAAAAACTAGAACTTGTGATCGTTGAACATGA
CAAATTGGAACGCGCACTTGAAGAGGTTTATGGTACAAACGCCCAGCTTGAATTTCTGAATAGTTTACATAATGAAGACA
GCGAAAATACTGAAGACATCTTAAGTGAGTTTAGCTTTGATATAAATGCAGACCATGAGAAAGATGACACTATTGTTGTT
AAATATTTAAATAAACTGCTTTATGATGCAATTAAAATGGGGGCATCCGACCTTCATTTTGAACCTTATGCCAAAATCTA
TCGCGTTAGATATCGTATCGATGGTGTTTTACATGCAATTTCATTCCCACCATTAGTACTCGCACCAAGACTAACTGTTC
GCCTAAAAATTATGGCGAAGATGGATATCTCAGAACGGCGCTCTCCACAAGATGGTCGTATAAAATTACCAGTCTCACAA
TTTAAATCTATCGATTTTCGTGTCAGCTCACTGCCTACATTATATGGTGAAAAATTAGTGCTACGTATTCTGGATGCTTC
TAGCGCAACATTGGGTATTGATGCGCTTGGCTACGAGGAAGATCAAAAAGCATTATTTTTAGAAGCACTGAACAAGCCAC
AAGGTATGTTGCTAATTACAGGACCAACGGGTTCTGGTAAAACAGTTTCGTTATACACAGGCCTCAATATTCTTAATACT
GAGAGTTCAAATATTTCCACAGCAGAAGATCCTGTGGAAATTAACCTTGAAGGTGTTAATCAGGTGAATGTTAACCCCAA
GGTGGGCTTGACCTTTGCCGCTGCGCTAAAGTCTTTTTTACGTCAGGATCCTGACATTATCATGGTTGGTGAGATTCGTG
ATCTGGAAACAGCAGAAATCGCAATCAAAGCCGCACAAACGGGTCACATGGTCATGTCTACGCTGCATACTAACAGTGCG
CCAGAAACATTAACACGTTTACGCAATATGGGCGTACCTTCATTTAATCTTGCAACGTCCGTGAACTTAGTAATTGCTCA
ACGATTGGCCCGACGTCTTTGTAGTAAATGTAAAGAGCCAATTCAGATCCCCAGAAATAGTTTGATCGAGATGGGGTTTA
CAGAGGAGGATCTATCTCACCCTAAACTCCGTATTTACCAACCTGTGGGATGTTCAGAATGCCGTGAAGGCTACAAAGGT
CGTGTCGGGATTTACGAAGTCATGAAAGTCACACCTGAAATTTCGAGAATTATTATGGAAGATGGAAATGCAATACAAAT
TGCAGATGCATCGGAAAAACTGGGGTTTAATAATCTTCGTCGCTCAGGTCTTAAAAAGGTGATGCAAGGGATTACTTCTT
TACAGGAAGTCAATCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(22-121)

Type II/IV secretion system protein (T2SSE)

(165-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

72.694

99.816

0.726
  pilB Acinetobacter baylyi ADP1

68.817

100

0.707
  pilB Legionella pneumophila strain ERS1305867

50.832

99.632

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

51.154

95.764

0.49
  pilF Neisseria gonorrhoeae MS11

48.624

100

0.488
  pilB Vibrio cholerae strain A1552

50

96.133

0.481
  pilB Vibrio campbellii strain DS40M4

51.292

92.634

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

37.838

95.396

0.361
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.773

98.158

0.361

Multiple sequence alignment