Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP88_RS05635 Genome accession   NZ_CP167212
Coordinates   1235907..1237643 (+) Length   578 a.a.
NCBI ID   WP_372163326.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. cyamopsidis strain NCPPB 3758     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230907..1242643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP88_RS05605 (ACCP88_05625) - 1232185..1232601 (-) 417 WP_372162900.1 pilin -
  ACCP88_RS05610 (ACCP88_05630) - 1232652..1232948 (+) 297 WP_372162902.1 hypothetical protein -
  ACCP88_RS05615 (ACCP88_05635) - 1232978..1233328 (+) 351 WP_372162904.1 SMR family transporter -
  ACCP88_RS05620 (ACCP88_05640) - 1233333..1234127 (+) 795 WP_277573650.1 glycosyltransferase -
  ACCP88_RS05625 (ACCP88_05645) - 1234142..1235170 (+) 1029 WP_372162906.1 glycosyltransferase -
  ACCP88_RS05630 (ACCP88_05650) - 1235163..1235810 (+) 648 WP_277573648.1 methionine biosynthesis protein MetW -
  ACCP88_RS05635 (ACCP88_05655) pilB 1235907..1237643 (+) 1737 WP_372163326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP88_RS05640 (ACCP88_05660) pilB 1237774..1237893 (+) 120 WP_029220459.1 hypothetical protein Machinery gene
  ACCP88_RS05645 (ACCP88_05665) pilR 1238074..1239528 (-) 1455 WP_206212849.1 sigma-54 dependent transcriptional regulator Regulator
  ACCP88_RS05650 (ACCP88_05670) - 1239795..1241408 (-) 1614 WP_372162909.1 ATP-binding protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62554.55 Da        Isoelectric Point: 5.4503

>NTDB_id=1038884 ACCP88_RS05635 WP_372163326.1 1235907..1237643(+) (pilB) [Xanthomonas axonopodis pv. cyamopsidis strain NCPPB 3758]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAAAAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSSFDPN
QNAIKLVSEELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNATQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1038884 ACCP88_RS05635 WP_372163326.1 1235907..1237643(+) (pilB) [Xanthomonas axonopodis pv. cyamopsidis strain NCPPB 3758]
ATGAATAGCGTCGTCACGGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
GGGCGCGGCGCGTGCTGCGATGGATCAGGCTGCTGCTGCAAAGGTCCCGTTGCCTCAATGGTTCTCGGAAAAAAAGTTGG
TGACAGCTTCGCAACTTGCGGCGGCCAACGCCGTGGAGTTTGGGATGCCGCTACTTGACGTGTCGTCGTTCGACCCTAAT
CAGAATGCAATTAAATTGGTTAGCGAAGAATTGCTGCAAAAGTTTCAAGTGCTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTAGGGGTGAGCAACCCGACCCAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTTGAGGCCA
TTCTTGTCGACGAGGATCAGATCCGTCGGACGCTCGAGCAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTAGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCT
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCTGAAGGATTCACGCCCGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGACGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCGGAAATCAATCGGGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilF Neisseria gonorrhoeae MS11

49.824

98.27

0.49
  pilB Vibrio cholerae strain A1552

50.181

95.848

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

46.786

96.886

0.453
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

36.617

100

0.386

Multiple sequence alignment