Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP84_RS15735 Genome accession   NZ_CP167201
Coordinates   3649109..3650842 (-) Length   577 a.a.
NCBI ID   WP_372170877.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. ricini strain NCPPB 2369     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3644109..3655842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP84_RS15720 (ACCP84_15770) - 3645155..3646768 (+) 1614 WP_372170875.1 PAS domain-containing sensor histidine kinase -
  ACCP84_RS15725 (ACCP84_15775) pilR 3647036..3648490 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  ACCP84_RS15730 (ACCP84_15780) pilB 3648712..3648831 (-) 120 WP_136732873.1 pilus assembly protein Machinery gene
  ACCP84_RS15735 (ACCP84_15785) pilB 3649109..3650842 (-) 1734 WP_372170877.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP84_RS15740 (ACCP84_15790) pilA2 3650980..3651408 (-) 429 WP_039587436.1 pilin Machinery gene
  ACCP84_RS15745 (ACCP84_15795) pilC 3651753..3653015 (+) 1263 WP_078515376.1 type II secretion system F family protein Machinery gene
  ACCP84_RS15750 (ACCP84_15800) - 3653022..3653885 (+) 864 WP_008576868.1 A24 family peptidase -
  ACCP84_RS15755 (ACCP84_15805) coaE 3653899..3654510 (+) 612 WP_277572157.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62453.55 Da        Isoelectric Point: 5.7600

>NTDB_id=1038837 ACCP84_RS15735 WP_372170877.1 3649109..3650842(-) (pilB) [Xanthomonas axonopodis pv. ricini strain NCPPB 2369]
MSAVAANLVGITGIARRLVQDGALDEASARVAMDQAAQAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLLDVSAFDASQ
NAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGN
DDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSTLPE
NALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1038837 ACCP84_RS15735 WP_372170877.1 3649109..3650842(-) (pilB) [Xanthomonas axonopodis pv. ricini strain NCPPB 2369]
ATGAGCGCAGTTGCTGCGAACTTAGTAGGAATCACAGGTATCGCGCGCCGCTTGGTGCAGGACGGGGCGCTGGACGAGGC
AAGTGCTCGTGTTGCCATGGATCAGGCCGCTCAGGCTAAGGTCCCGCTGCCGCAGTGGTTCGCTGAAAAGAAGCTTGTGT
CGGCGGCGCAGCTAGCTGCAGCGAATGCTGTCGAGTTTGGCATGCCACTGCTCGATGTTTCCGCATTCGACGCCAGCCAG
AACGCAGTAAAGCTCGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAAGCCATTC
TTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCCTTGGGTTCGTCGCTTGGCAAC
GACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGATATGGGCGCCGGCGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACATCC
ATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGACTGCTGAAGAACGTGGCCAAGGCGCCGGTG
AAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAAAGG
TGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTG
TTCCTTGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCTGTA
TACGGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCTTGCCAGGCG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCT
TTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATTGCGCCCTACAACATCACTT
CGTCGGTGACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAGTCGACGCTGCCGGAG
AATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCCGCCGGGATCGAGCTGTACGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATGAAG
GCTGCCCACGGGGTTACCAGCCTGGCCGAGATCAATCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

98.44

0.555
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

52.166

100

0.522
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio cholerae strain A1552

50.638

95.147

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

97.054

0.451
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

91.681

0.362

Multiple sequence alignment