Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACBZ91_RS09895 Genome accession   NZ_CP167179
Coordinates   2140494..2142179 (+) Length   561 a.a.
NCBI ID   WP_372377299.1    Uniprot ID   -
Organism   Vibrio natriegens strain JSH01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2135494..2147179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACBZ91_RS09875 (ACBZ91_09875) pdhR 2137094..2137861 (-) 768 WP_014232947.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACBZ91_RS09880 (ACBZ91_09880) ampD 2138272..2138817 (-) 546 WP_065302862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACBZ91_RS09885 (ACBZ91_09885) nadC 2138910..2139797 (+) 888 WP_372377297.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACBZ91_RS09890 (ACBZ91_09890) - 2140054..2140491 (+) 438 WP_372377298.1 pilin -
  ACBZ91_RS09895 (ACBZ91_09895) pilB 2140494..2142179 (+) 1686 WP_372377299.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACBZ91_RS09900 (ACBZ91_09900) pilC 2142210..2143433 (+) 1224 WP_372377300.1 type II secretion system F family protein Machinery gene
  ACBZ91_RS09905 (ACBZ91_09905) pilD 2143501..2144370 (+) 870 WP_372377301.1 A24 family peptidase Machinery gene
  ACBZ91_RS09910 (ACBZ91_09910) coaE 2144371..2145015 (+) 645 WP_372377302.1 dephospho-CoA kinase -
  ACBZ91_RS09915 (ACBZ91_09915) zapD 2145012..2145752 (+) 741 WP_014232955.1 cell division protein ZapD -
  ACBZ91_RS09920 (ACBZ91_09920) yacG 2145826..2146020 (+) 195 WP_014232956.1 DNA gyrase inhibitor YacG -
  ACBZ91_RS09925 (ACBZ91_09925) rplS 2146346..2146699 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62155.93 Da        Isoelectric Point: 5.8384

>NTDB_id=1038681 ACBZ91_RS09895 WP_372377299.1 2140494..2142179(+) (pilB) [Vibrio natriegens strain JSH01]
MYTNLSTILRQSGLLSLAQEESLVEQVNASGISMPEALVNSGIFTTRELASHLRSIFGLPYEPLSNYDYTALCQKLGLRE
LITSHHALPLERTPSTLTLAIADPTNQQAEDDFRFATGLQVDLVIVDFSELDAAIRRLYGRSLGQERSGLKEINQEELAN
LVDVGADEVDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRMPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLTNMGVESYNLSSSLSLIIAQRLARKLCPHCKQPQEVTAALQHIGINATDQ
IYQANPNGCNHCTHGYSGRTGIYEVMRFDETLSEALIKGASVNQLEKLAIRNGMSTLQMSGIKKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1038681 ACBZ91_RS09895 WP_372377299.1 2140494..2142179(+) (pilB) [Vibrio natriegens strain JSH01]
ATGTACACAAACCTCTCAACGATTCTTCGCCAATCCGGGTTACTTAGCTTAGCCCAAGAAGAATCGTTAGTAGAGCAAGT
CAACGCTTCCGGTATTTCGATGCCGGAAGCGTTAGTCAATTCTGGTATATTCACTACTAGAGAACTCGCTAGTCACCTAA
GATCGATATTTGGTTTACCCTACGAACCATTATCTAATTATGATTACACCGCGCTGTGCCAAAAATTGGGGCTACGTGAA
CTGATCACCAGTCACCATGCATTGCCACTGGAGCGAACGCCTTCGACGTTAACTCTCGCCATTGCCGACCCAACGAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCGACAGGATTACAGGTTGATCTTGTCATCGTAGATTTTTCAGAACTTGATGCTG
CAATTCGCCGTTTGTACGGTCGCTCGTTAGGTCAGGAGAGATCGGGCTTAAAAGAGATCAACCAAGAAGAACTCGCCAAC
CTTGTCGACGTTGGCGCAGATGAAGTCGACAACATCGAAGACTTAAGTCAGGACGAGTCACCGGTTAGTCGCTATATCAA
CCAAATTTTGCTTGATGCTGTCCGTAAAGGCGCATCTGATATCCACTTTGAACCTTATGAAAAAATGTACCGGGTTCGCC
TGCGCTGCGACGGCATTTTGATTGAAACCCAACAACCACCAAGCCATTTAAGTCGACGCTTATCAGCGCGAATCAAAATT
CTATCTAAACTGGATATTGCCGAAAGGCGTATGCCGCAAGACGGTCGAATAAAGTTAAAGCTTAATCAAGACACTGCGAT
TGATATGCGAGTGTCCACCTTACCGACTCTGTTCGGAGAAAAAATCGTATTACGACTGCTCGACAGCAGCTCAGCTTCTC
TGGATATAGATAAACTAGGCTACAGTGACCAGCAAAAGCAGCTGTACTTAGATGCTCTGCGTCGTCCACAAGGCATGATT
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTCTCGTTGTACACGGGGCTGAGCATACTCAATAAACCAGAGATCAA
TATTTCTACAGCTGAAGACCCGGTCGAAATCAATCTCTCCGGAATTAATCAGGTTCAGGTTCAACCCAAGATAGGTTTTG
GTTTCGCGGAGGCACTGCGCTCTTTCCTGCGTCAAGATCCTGATGTGGTCATGGTGGGAGAAATCCGCGATTTGGACACA
GCAGAAATCGCGATTAAGGCCTCACAAACCGGACACTTAGTCTTGTCGACACTCCACACAAATTCAGCCGCTGAAACCGT
TATCCGCCTAACGAATATGGGCGTTGAAAGCTACAACCTCTCCTCGTCGCTCAGCCTTATTATTGCCCAACGCTTAGCCC
GTAAACTTTGCCCGCACTGTAAACAACCTCAAGAGGTTACCGCCGCATTACAACATATTGGCATTAACGCAACTGACCAG
ATTTATCAAGCCAACCCAAATGGCTGTAACCACTGTACACACGGCTATTCGGGAAGAACTGGCATCTATGAGGTGATGCG
TTTCGATGAAACCCTATCCGAAGCACTGATTAAAGGTGCCTCGGTAAATCAACTCGAAAAACTCGCCATCCGTAACGGGA
TGAGCACACTACAAATGTCAGGTATTAAAAAGCTTAAGCAAGGCGTCACAAGCTTTAGCGAACTGCAGCGCGTGCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

88.057

100

0.881
  pilB Vibrio campbellii strain DS40M4

85.561

100

0.856
  pilB Vibrio cholerae strain A1552

72.42

100

0.725
  pilB Acinetobacter baumannii D1279779

51.248

92.87

0.476
  pilB Legionella pneumophila strain ERS1305867

48.614

96.435

0.469
  pilB Acinetobacter baylyi ADP1

50.884

90.731

0.462
  pilF Neisseria gonorrhoeae MS11

43.972

100

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

36.242

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.786

99.822

0.367
  pilF Thermus thermophilus HB27

36.655

100

0.367

Multiple sequence alignment