Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACB088_RS14560 Genome accession   NZ_CP167121
Coordinates   3080196..3081908 (+) Length   570 a.a.
NCBI ID   WP_375037297.1    Uniprot ID   -
Organism   Acinetobacter sp. RW6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3075196..3086908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB088_RS14525 (ACB088_14525) nusA 3076082..3077566 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  ACB088_RS14530 (ACB088_14530) rimP 3077604..3078128 (-) 525 WP_375037296.1 ribosome maturation factor RimP -
  ACB088_RS14550 (ACB088_14550) secG 3078768..3079097 (-) 330 WP_092710024.1 preprotein translocase subunit SecG -
  ACB088_RS14555 (ACB088_14555) tpiA 3079110..3079904 (-) 795 WP_003654580.1 triose-phosphate isomerase -
  ACB088_RS14560 (ACB088_14560) pilB 3080196..3081908 (+) 1713 WP_375037297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACB088_RS14565 (ACB088_14565) pilC 3081936..3083162 (+) 1227 WP_198496440.1 type II secretion system F family protein Machinery gene
  ACB088_RS14570 (ACB088_14570) pilD 3083162..3084022 (+) 861 WP_375037298.1 prepilin peptidase Machinery gene
  ACB088_RS14575 (ACB088_14575) coaE 3084024..3084620 (+) 597 WP_375037299.1 dephospho-CoA kinase -
  ACB088_RS14580 (ACB088_14580) - 3084617..3085531 (-) 915 WP_375037300.1 DMT family transporter -
  ACB088_RS14585 (ACB088_14585) rlmB 3085567..3086316 (-) 750 WP_004640838.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACB088_RS14590 (ACB088_14590) - 3086419..3086745 (-) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63488.86 Da        Isoelectric Point: 5.0204

>NTDB_id=1038293 ACB088_RS14560 WP_375037297.1 3080196..3081908(+) (pilB) [Acinetobacter sp. RW6]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVSYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLVQRGQILYVAISNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLDTSTAPEEDDDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKLPSDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1038293 ACB088_RS14560 WP_375037297.1 3080196..3081908(+) (pilB) [Acinetobacter sp. RW6]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACAGGATACAGTTTCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGATCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAATCAGTAATCCTAGTAACATTGAAGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAACCGGTTATTG
TCGAGCACCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGTAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGATACTTCAACCGCCCCAGAAGAGGATGATGACACTCCTCAGGGCGATGAAGCACCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGGGCTTCAGACTTGCATTTCGAACCCTATGAGAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAGATTGCAAATCCGCCTTTGCAACTGGCTAACCGTTTGGCTTCA
CGCTTAAAAGTGATGTCTCAAATGGACATTTCTGAAAAGCGTGTCCCTCAAGATGGTCGTATCAAACTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACTTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CTAGTGCCATGCTGGGAATTGATGCTCTAGGATATGAAGAAGATCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCCTTATATACAGGCCTCAACATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTGAACCCTA
AAGTAGGTCTTACTTTTTCGGCTGCGCTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCTGCTCAGACGGGCCATATGGTAATGTCCACACTACATACTAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTTAATATTGCGACATCGGTCAATTTGGTTATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGCTGCCTAGTGACATTCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACAGAACAAGATCTAACACATCCCGATTTTCAAGTTTTTCAACCGGTCGGTTGTCCTGAATGTCGTGAGGGTTACAAAGG
TCGAGTAGGTATTTATGAGGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTGCGTCGCTCAGGTTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.386

100

0.944
  pilB Acinetobacter baylyi ADP1

78.947

100

0.789
  pilB Legionella pneumophila strain ERS1305867

52.014

100

0.521
  pilF Neisseria gonorrhoeae MS11

48.932

98.596

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.504

93.333

0.481
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB Vibrio cholerae strain A1552

49.711

91.053

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384

Multiple sequence alignment