Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACA087_RS20655 Genome accession   NZ_CP166975
Coordinates   4576236..4578308 (-) Length   690 a.a.
NCBI ID   WP_371354974.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain Pc01-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4571236..4583308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA087_RS20640 (ACA087_20635) - 4572864..4574453 (-) 1590 WP_016704448.1 SPOR domain-containing protein -
  ACA087_RS20645 (ACA087_20640) aroB 4574464..4575564 (-) 1101 WP_371354973.1 3-dehydroquinate synthase -
  ACA087_RS20650 (ACA087_20645) aroK 4575713..4576231 (-) 519 WP_009041824.1 shikimate kinase AroK -
  ACA087_RS20655 (ACA087_20650) pilQ 4576236..4578308 (-) 2073 WP_371354974.1 type IV pilus secretin PilQ Machinery gene
  ACA087_RS20660 (ACA087_20655) - 4578322..4578849 (-) 528 WP_371354975.1 pilus assembly protein PilP -
  ACA087_RS20665 (ACA087_20660) - 4578846..4579463 (-) 618 WP_371354976.1 type 4a pilus biogenesis protein PilO -
  ACA087_RS20670 (ACA087_20665) - 4579460..4580026 (-) 567 WP_371354977.1 PilN domain-containing protein -
  ACA087_RS20675 (ACA087_20670) pilM 4580026..4581078 (-) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74640.77 Da        Isoelectric Point: 5.4337

>NTDB_id=1036503 ACA087_RS20655 WP_371354974.1 4576236..4578308(-) (pilQ) [Pseudomonas chlororaphis strain Pc01-6]
MNRIFSAFGIALWMALLSPMVMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYATDQPARIALDLPGVVSQLANKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTISVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=1036503 ACA087_RS20655 WP_371354974.1 4576236..4578308(-) (pilQ) [Pseudomonas chlororaphis strain Pc01-6]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGTCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCGCGGG
GTTACGCCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGTTGGCGAACAAGAGCCGCGAT
CTGGGCAATGGCAACGCTCGCAGCGTGACAGTGGTCGAATCGGGACAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCCGCGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGTGAGGGCAACGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACCC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGATAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAATGCCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATAGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGTGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCGCTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAAATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGT
GGCTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGC
TCTTCCACCATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACTGACAACGTGTTGCTGGACCTTGAACTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTTACCTCCGACAAAGAGACGGCGAAGATTCTCAAAGGTACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCAACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(298-344)

AMIN domain (AMIN)

(40-122)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.732
  comQ Acinetobacter baylyi ADP1

41.439

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.412
  pilQ Legionella pneumophila strain Lp02

37.89

97.536

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.967

96.957

0.368

Multiple sequence alignment