Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACA087_RS20675 Genome accession   NZ_CP166975
Coordinates   4580026..4581078 (-) Length   350 a.a.
NCBI ID   WP_028683780.1    Uniprot ID   A0AAJ0ZMY7
Organism   Pseudomonas chlororaphis strain Pc01-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4575026..4586078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA087_RS20650 (ACA087_20645) aroK 4575713..4576231 (-) 519 WP_009041824.1 shikimate kinase AroK -
  ACA087_RS20655 (ACA087_20650) pilQ 4576236..4578308 (-) 2073 WP_371354974.1 type IV pilus secretin PilQ Machinery gene
  ACA087_RS20660 (ACA087_20655) - 4578322..4578849 (-) 528 WP_371354975.1 pilus assembly protein PilP -
  ACA087_RS20665 (ACA087_20660) - 4578846..4579463 (-) 618 WP_371354976.1 type 4a pilus biogenesis protein PilO -
  ACA087_RS20670 (ACA087_20665) - 4579460..4580026 (-) 567 WP_371354977.1 PilN domain-containing protein -
  ACA087_RS20675 (ACA087_20670) pilM 4580026..4581078 (-) 1053 WP_028683780.1 pilus assembly protein PilM Machinery gene
  ACA087_RS20680 (ACA087_20675) - 4581295..4583730 (+) 2436 WP_164485365.1 penicillin-binding protein 1A -
  ACA087_RS20685 (ACA087_20680) - 4583858..4585126 (-) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37047.51 Da        Isoelectric Point: 5.0008

>NTDB_id=1036504 ACA087_RS20675 WP_028683780.1 4580026..4581078(-) (pilM) [Pseudomonas chlororaphis strain Pc01-6]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=1036504 ACA087_RS20675 WP_028683780.1 4580026..4581078(-) (pilM) [Pseudomonas chlororaphis strain Pc01-6]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGTCTGCTGGCCAGGGCCCGGCCGGGAGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCCTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGGCTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.557

100

0.529
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment