Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACA097_RS14855 Genome accession   NZ_CP166971
Coordinates   3155166..3157253 (+) Length   695 a.a.
NCBI ID   WP_160288625.1    Uniprot ID   -
Organism   Pseudomonas sp. QL9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3150166..3162253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA097_RS14835 (ACA097_14830) comM 3152247..3153311 (+) 1065 WP_371364173.1 pilus assembly protein PilM Machinery gene
  ACA097_RS14840 (ACA097_14835) pilN 3153311..3153910 (+) 600 WP_043255938.1 type 4a pilus biogenesis protein PilN -
  ACA097_RS14845 (ACA097_14840) pilO 3153907..3154587 (+) 681 WP_052355394.1 type 4a pilus biogenesis protein PilO -
  ACA097_RS14850 (ACA097_14845) pilP 3154584..3155108 (+) 525 WP_043255936.1 type 4a pilus biogenesis lipoprotein PilP -
  ACA097_RS14855 (ACA097_14850) pilQ 3155166..3157253 (+) 2088 WP_160288625.1 type IV pilus secretin PilQ Machinery gene
  ACA097_RS14860 (ACA097_14855) aroK 3157262..3157780 (+) 519 WP_371364176.1 shikimate kinase AroK -
  ACA097_RS14865 (ACA097_14860) aroB 3157832..3158938 (+) 1107 WP_043255932.1 3-dehydroquinate synthase -
  ACA097_RS14870 (ACA097_14865) - 3158945..3160618 (+) 1674 WP_371364178.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74958.99 Da        Isoelectric Point: 5.5504

>NTDB_id=1036479 ACA097_RS14855 WP_160288625.1 3155166..3157253(+) (pilQ) [Pseudomonas sp. QL9]
MNRTMTRLGVALLVGLFAPHLLAADLQSLDVASLPGDRVELKLGFDQPVVAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSVTVVEAKDRTRLIINLGTLVPYSTRTQGNNLFILVGQGTGASAPVASATPAATSYASSARPAQPKPYIPAGK
AIRNIDFQRGDQGEGNVVIDLSDPGVSPDIQEQGGKIRLNFPKTQLPESLRVRLDVKDFATPVQFVNASEAGGNATINIE
PSGLFDYLVYQTDNRMTVSIKPLSAGDAEKRKKDTFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVTG
SATGRGASAATANTDNRGSITVDDRTNSIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVNWSGSL
SNRGNWNVSGGTDSTSQTFVDLGVSTTTPTSGIGIGFVTNNTVLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTAKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1036479 ACA097_RS14855 WP_160288625.1 3155166..3157253(+) (pilQ) [Pseudomonas sp. QL9]
ATGAACAGAACGATGACCCGCCTGGGCGTTGCCCTGCTGGTAGGCCTTTTTGCACCGCATCTGCTTGCGGCCGACCTGCA
GAGCCTTGACGTGGCCTCGCTGCCGGGCGATCGCGTGGAGCTCAAGCTCGGCTTCGACCAGCCTGTGGTGGCGCCGCGCG
GCTATACCATCGAGCAACCCGCGCGTATCGCGCTGGATCTGCCTGGTGTGCAGAACAAACTGGGCAGCAAGAACCGCGAG
TTGGGCGTGGGTAACGCCCGCAGCGTGACGGTTGTCGAGGCCAAGGATCGAACCCGGTTGATCATCAACTTGGGCACGCT
GGTGCCGTATTCGACCCGGACCCAGGGCAACAACCTGTTCATTCTCGTCGGCCAGGGCACTGGAGCTTCCGCACCCGTTG
CCAGTGCAACGCCGGCGGCGACCAGCTATGCCTCTTCGGCTCGTCCGGCGCAGCCCAAGCCTTACATTCCGGCCGGCAAG
GCGATTCGCAACATCGACTTCCAGCGCGGTGATCAGGGTGAAGGCAATGTCGTCATCGACCTCTCCGATCCTGGTGTCAG
CCCGGACATTCAGGAGCAAGGCGGCAAGATTCGCCTGAATTTCCCCAAGACGCAGCTTCCTGAAAGCCTGCGTGTGCGCC
TGGACGTGAAGGACTTCGCCACTCCGGTGCAGTTCGTCAACGCTTCGGAAGCGGGTGGAAACGCCACCATCAACATCGAG
CCGAGCGGTCTGTTCGATTATCTGGTGTATCAGACCGACAACCGCATGACCGTGAGCATCAAACCGCTCAGCGCCGGTGA
TGCCGAGAAGCGCAAGAAGGACACATTCGCCTACACCGGCGAGAAGCTGTCGCTCAACTTCCAGGACATCGACGTACGCT
CCGTCCTGCAGCTTATCGCCGACTTCACCGACCTGAACCTGGTGGCTTCCGATACTGTGCAGGGCAACATCACCCTGCGC
CTGCAGAACGTGCCCTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGT
GCTTCTGGTGGCGCCGGCTGACGAGATCGCCGCTCGCGAGCGCCAGGAGCTGGAGGCGCAGAAACAGATTGCCGAACTGG
CGCCCCTGCGTCGTGAGTTGATCCAGGTGAACTACGCCAAGGCGTCTGATATTGCCAAGCTGTTCCAGTCGGTTACCGGT
AGCGCTACCGGTCGTGGTGCTTCGGCCGCGACCGCGAACACCGATAACCGCGGTTCGATCACCGTGGATGATCGCACCAA
CTCGATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGTATCGTTTCGCAGCTCGACATCCCTGTGCGCC
AGGTGATGATCGAGGCTCGTATCGTCGAGGCGAGCGTCGATTACACCAAGGAGCTGGGTGTGAACTGGAGCGGCTCGCTG
AGCAATCGGGGCAACTGGAACGTTTCCGGTGGCACCGACAGCACCTCGCAAACCTTCGTTGACCTGGGCGTGAGCACTAC
TACTCCGACGTCCGGTATCGGCATCGGCTTCGTGACCAACAACACCGTTCTCGATCTTCAGCTCAACGCGATGGAAAAGA
CCGGTAACGGTGAAGTGGTATCGCAGCCCAAGGTGGTCACCTCGGACAAGGAGACCGCGAAGATCCTGAAAGGTACCGAG
GTTCCGTACCAGGAAGCGAGCTCCAGCGGCGCGACCTCGGTTTCCTTCAAGGAGGCCGCACTGTCTCTGGAAGTCACTCC
GCAGATCACTCCTGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCGGACTACCAGAACGCGCTGCTCG
GTGTGCCTCCGATCAAGAAGAACGAGGTCAACGCCAAGGTCCTGGTCAATGACGGCGAGACCATCGTTATCGGTGGTGTG
TTCTCCAACACTCAGACCAAGACGACCGCGAAGGTACCGTTCCTGGGTGATGTGCCGGTAGTCGGTCGCCTGTTCAAGAA
TGACTACGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCTATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.105

100

0.793
  comQ Acinetobacter baylyi ADP1

42.052

99.568

0.419
  pilQ Acinetobacter baumannii D1279779

40.647

100

0.416
  pilQ Acinetobacter baumannii strain A118

40.647

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.563

98.129

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.563

98.129

0.378

Multiple sequence alignment