Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAX20_RS02040 Genome accession   NZ_CP166919
Coordinates   464982..466679 (-) Length   565 a.a.
NCBI ID   WP_371188165.1    Uniprot ID   -
Organism   Thalassotalea maritima strain Sam97     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459982..471679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAX20_RS02020 - 461216..461962 (-) 747 WP_371188159.1 cell division protein ZapD -
  ACAX20_RS02025 coaE 462073..462687 (-) 615 WP_371189552.1 dephospho-CoA kinase -
  ACAX20_RS02030 pilD 462731..463615 (-) 885 WP_371188161.1 A24 family peptidase Machinery gene
  ACAX20_RS02035 pilC 463706..464956 (-) 1251 WP_371188163.1 type II secretion system F family protein Machinery gene
  ACAX20_RS02040 pilB 464982..466679 (-) 1698 WP_371188165.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAX20_RS02045 - 466774..467184 (-) 411 WP_371188167.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACAX20_RS02050 - 467229..467654 (-) 426 WP_371188169.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACAX20_RS02055 nadC 468275..469168 (-) 894 WP_371188171.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACAX20_RS02060 - 469477..469992 (-) 516 WP_371188172.1 TIGR02281 family clan AA aspartic protease -
  ACAX20_RS02065 ampD 470193..470747 (+) 555 WP_371188174.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACAX20_RS02070 ampE 470802..471647 (+) 846 WP_371188176.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62017.04 Da        Isoelectric Point: 4.8094

>NTDB_id=1036196 ACAX20_RS02040 WP_371188165.1 464982..466679(-) (pilB) [Thalassotalea maritima strain Sam97]
MKGIHRQSPILSAAVKSNLISKEQAADIFELCQKQQVCLLSHINKEQIIKPEALARVLSKSFALPITSLKSIDLDNLPDG
LRDEKLIKKHNALPIYLRGGILFVAVADPTNIDALEEFQFNTGYNTEMVLADYEQLQKTIEQLLEDDHSALAITDLDAAE
LGNIDVEDTEKEDDADGGENDAPIVVYINKILLDAIKRGASDLHFEPFEKTFRIRFRVDGILTEIATPPVNLASRMTARL
KVMSKLDIAERRVPQDGRIKLAVTKNKSIDFRVSTLPTMWGEKVVMRILDSSSAKLGIDILGYEDDQKALYMDALSKPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINNKAGLTFAEALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNKCKEVDDISEQALIEFGFEP
SEVADIQIYKPIGCDECTKGYKGRVGIYEVMKISQDTATIIMDGGSSLDIAAQAQKEGYNNLRQSALLKAKSGVTSLAEV
SRVTA

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1036196 ACAX20_RS02040 WP_371188165.1 464982..466679(-) (pilB) [Thalassotalea maritima strain Sam97]
ATGAAAGGTATTCACCGCCAATCACCGATCTTGTCTGCGGCAGTAAAAAGTAACTTAATTAGCAAAGAGCAAGCTGCCGA
TATTTTCGAGCTTTGCCAAAAGCAACAAGTGTGCTTATTGTCGCATATCAATAAAGAGCAGATTATAAAACCTGAAGCTC
TTGCCAGAGTTTTATCGAAGTCTTTTGCATTGCCTATTACTAGCCTCAAGTCAATTGACTTAGATAACTTGCCCGATGGT
TTACGTGACGAAAAACTCATTAAGAAGCACAACGCGTTACCTATATACTTGCGTGGTGGCATTTTATTCGTTGCCGTTGC
TGACCCTACAAATATCGATGCACTAGAAGAGTTTCAATTTAATACAGGTTACAACACCGAGATGGTGTTGGCTGATTACG
AGCAGTTACAAAAAACGATTGAGCAACTGCTTGAAGATGATCATTCCGCATTGGCAATAACCGATTTAGATGCTGCTGAG
TTAGGTAACATCGATGTTGAAGATACCGAAAAAGAAGACGACGCAGATGGCGGAGAAAATGATGCGCCTATTGTTGTCTA
CATCAATAAAATTCTATTAGATGCGATTAAACGCGGCGCATCCGATTTACACTTTGAGCCTTTTGAAAAGACCTTTAGAA
TTCGCTTTCGTGTCGATGGTATTTTAACCGAAATCGCCACACCGCCAGTTAACCTAGCGAGCCGTATGACAGCCCGCTTA
AAAGTAATGTCAAAACTAGACATTGCCGAGCGCCGAGTACCACAAGATGGTCGTATAAAATTAGCGGTTACTAAAAACAA
ATCCATTGATTTTCGTGTTTCCACCCTGCCAACTATGTGGGGCGAAAAAGTGGTAATGCGTATACTAGACTCCTCAAGTG
CCAAACTGGGTATTGATATTTTAGGTTATGAAGACGACCAAAAAGCACTTTATATGGATGCGCTATCTAAACCGCAAGGC
ATGATTCTAGTTACTGGTCCAACGGGTTCTGGTAAAACCGTATCCTTGTATACTGGCTTAAATATTCTAAATACAGAAGA
GCGCAATATATCAACCGCCGAAGACCCTGTGGAGATTAACCTTGAGGGCGTTAACCAGGTTCAAATTAACAATAAGGCGG
GTTTGACGTTTGCCGAAGCACTGAAATCGTTCCTACGCCAAGATCCCGACATTGTCATGGTTGGTGAGATCCGTGATTTA
GAAACCGCAGAAATCGCTATTAAAGCCGCACAAACAGGTCACTTGGTTTTATCTACACTGCACACTAACTCTGCGGCAGA
AACACTTACACGTCTTTTAAATATGGGTGTTCCCTCTTATAACGTTGCAAGTTCGGTAAGCTTAATAATCGCTCAACGTC
TTGCTCGAAGACTTTGCAATAAGTGCAAAGAAGTGGATGACATATCGGAGCAAGCCCTTATAGAATTTGGCTTTGAGCCC
AGCGAAGTTGCCGATATACAAATATACAAGCCTATAGGTTGTGATGAATGTACTAAAGGTTATAAAGGCCGTGTCGGTAT
ATATGAAGTAATGAAAATCAGTCAAGATACTGCAACCATCATTATGGATGGTGGTAGCTCACTGGATATTGCTGCTCAAG
CTCAAAAAGAAGGCTACAACAACTTGCGACAATCGGCGTTATTGAAAGCAAAAAGTGGCGTTACCTCACTGGCAGAAGTA
AGTCGTGTAACGGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.774

94.159

0.563
  pilB Acinetobacter baylyi ADP1

59.038

92.035

0.543
  pilB Legionella pneumophila strain ERS1305867

53.982

100

0.54
  pilB Vibrio campbellii strain DS40M4

52.91

100

0.531
  pilB Vibrio cholerae strain A1552

52.931

99.646

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

52.855

96.106

0.508
  pilF Neisseria gonorrhoeae MS11

48.571

99.115

0.481
  pilF Thermus thermophilus HB27

36.126

100

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

93.097

0.363

Multiple sequence alignment