Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUF19_RS14235 Genome accession   NZ_AP028067
Coordinates   3222107..3223792 (+) Length   561 a.a.
NCBI ID   WP_286294726.1    Uniprot ID   -
Organism   Vibrio sp. FE10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3217107..3228792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF19_RS14215 pdhR 3218606..3219367 (-) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QUF19_RS14220 ampD 3219851..3220399 (-) 549 WP_286294724.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUF19_RS14225 nadC 3220535..3221425 (+) 891 WP_102437684.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUF19_RS14230 pilA 3221681..3222106 (+) 426 WP_286294725.1 pilin Machinery gene
  QUF19_RS14235 pilB 3222107..3223792 (+) 1686 WP_286294726.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUF19_RS14240 pilC 3223807..3225036 (+) 1230 WP_286294727.1 type II secretion system F family protein Machinery gene
  QUF19_RS14245 pilD 3225105..3225974 (+) 870 WP_286294728.1 A24 family peptidase Machinery gene
  QUF19_RS14250 coaE 3225977..3226591 (+) 615 WP_286294730.1 dephospho-CoA kinase -
  QUF19_RS14255 zapD 3226618..3227358 (+) 741 WP_017108712.1 cell division protein ZapD -
  QUF19_RS14260 yacG 3227440..3227637 (+) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  QUF19_RS14265 rplS 3228152..3228505 (-) 354 WP_009847607.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62022.66 Da        Isoelectric Point: 5.0429

>NTDB_id=103522 QUF19_RS14235 WP_286294726.1 3222107..3223792(+) (pilB) [Vibrio sp. FE10]
MLTNLPTVLRQANLLSLAQEQAVAEHVHASGVSTPEALLALDIFTSESLAHNIQAIFGLPPVQLANTDYEVLCDQLGLRE
LITKYHAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEERFRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVAPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADDSPDIFLRHSIPNSQT
IYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=103522 QUF19_RS14235 WP_286294726.1 3222107..3223792(+) (pilB) [Vibrio sp. FE10]
GTGCTCACCAACCTCCCAACCGTTCTACGTCAGGCTAACCTTCTTAGCCTGGCTCAAGAACAAGCCGTTGCGGAACATGT
ACATGCTTCAGGTGTTTCTACCCCTGAAGCTTTGCTCGCTCTAGACATCTTCACTAGCGAGAGTCTCGCGCATAACATTC
AGGCCATCTTCGGTCTGCCACCGGTGCAACTCGCCAATACTGACTACGAAGTCTTATGCGACCAATTAGGGCTTCGCGAG
CTGATCACTAAATATCACGCTATTCCGATTTCAGTATCTAGTTCGACTCTCACTCTCGCCTCAGCTGATCCAACCGACTT
ACAAGCAGAAGATGATTTCCGATTCGCGACCGGATTGCAGATAGAACTCGTTGTCGCTAACTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGACGTTCGATTTCCGGGCAAGACTCCAAGCGTAAAGAGATCACCCAAGATGAACTCGCCAAC
CTGGTTGAAGTCTCTGATGATGAGATGACTTCAATTGAAGATCTCAGCCAAGACGATTCTCCTGTTAGCCGTTTTATCAA
TCAAATACTGGTTGATGCGGTGCGTAAAGGTGCATCGGATATCCACTTTGAACCTTATGAAGAACGCTTCCGAGTACGAC
TGCGCTGTGATGGTATCCTTGTTGAAATACAACAACCCGCTTCCCACCTAAGTCGCCGTTTATCAGCTCGCTTAAAGATC
CTCGCCAAGCTGGATATCGCTGAGCGTCGCTTACCTCAAGATGGTCGTATCAAGTTGCGTTTAAATGACGAACTCGCGAT
TGATATGCGGGTTTCTACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTGCGTCTTTTAGATAGCAGCGCGGCCAATC
TAGATATCGATAAGTTGGGTTACAGCGAAGATCAAAAAGCACTCTATCTCAATGCATTGAAACGCCCACAAGGTATGATC
TTGATGACCGGACCAACCGGCAGTGGGAAAACCGTTTCTCTCTATACTGGCCTTCGAGTGCTTAACACCACTGAGCGCAA
CATCTCAACAGCTGAAGATCCTGTCGAAATCAATCTATGTGGGATCAATCAAGTTCAAGTCGCACCCAAAATCGGTTTTG
GATTTGCTGAAGCATTGCGATCATTTTTACGACAAGATCCCGATGTGGTCATGGTCGGTGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACCGGACACCTCGTTCTTTCGACACTGCATACCAACTCCGCTGCAGAAACCGT
GACTCGACTCGCTCACATGGGCATTGAACCCTTTAATCTTGCTTCATCACTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGTAACCACTGTAAAGCCGCTGACGACTCTCCGGACATATTTCTGCGCCACTCTATCCCTAACAGCCAAACG
ATCTACAAAGCCAATCCACAAGGGTGTAATGAATGTAACCAGGGATACTCTGGGCGAGTGGGGATTTATGAAGTTATGCC
GTTTAGCGATCAATTAAAAAACAGCCTGATCGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCGCGCCGAGAAGGCA
TGCGAACACTACAAGAATCAGGGCTAGATAAATTGTTGGAAGGCACCACCAGCTATCAAGAACTACAACGTGTTCTGTAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.582

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Acinetobacter baumannii D1279779

50.867

92.513

0.471
  pilB Legionella pneumophila strain ERS1305867

48.327

95.9

0.463
  pilB Acinetobacter baylyi ADP1

48.555

92.513

0.449
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433

Multiple sequence alignment