Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACA027_RS04195 Genome accession   NZ_CP166730
Coordinates   963209..964948 (-) Length   579 a.a.
NCBI ID   WP_370681150.1    Uniprot ID   -
Organism   Comamonas sp. GB3 AK4-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 958209..969948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA027_RS04165 (ACA027_04165) - 958709..959209 (+) 501 WP_370681144.1 NUDIX domain-containing protein -
  ACA027_RS04170 (ACA027_04170) - 959320..959535 (-) 216 WP_370681145.1 DNA gyrase inhibitor YacG -
  ACA027_RS04175 (ACA027_04175) zapD 959575..960330 (-) 756 WP_370681146.1 cell division protein ZapD -
  ACA027_RS04180 (ACA027_04180) coaE 960432..961043 (-) 612 WP_370681147.1 dephospho-CoA kinase -
  ACA027_RS04185 (ACA027_04185) pilD 961043..961918 (-) 876 WP_370681148.1 A24 family peptidase Machinery gene
  ACA027_RS04190 (ACA027_04190) pilC 961918..963159 (-) 1242 WP_370681149.1 type II secretion system F family protein Machinery gene
  ACA027_RS04195 (ACA027_04195) pilB 963209..964948 (-) 1740 WP_370681150.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACA027_RS04205 (ACA027_04205) - 965200..966129 (-) 930 WP_370682507.1 polyprenyl synthetase family protein -
  ACA027_RS04210 (ACA027_04210) rplU 966346..966657 (+) 312 WP_370682508.1 50S ribosomal protein L21 -
  ACA027_RS04215 (ACA027_04215) rpmA 966676..966933 (+) 258 WP_066540535.1 50S ribosomal protein L27 -
  ACA027_RS04220 (ACA027_04220) cgtA 967129..968247 (+) 1119 WP_370681151.1 Obg family GTPase CgtA -
  ACA027_RS04225 (ACA027_04225) proB 968379..969518 (+) 1140 WP_370681152.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62780.13 Da        Isoelectric Point: 7.2719

>NTDB_id=1035052 ACA027_RS04195 WP_370681150.1 963209..964948(-) (pilB) [Comamonas sp. GB3 AK4-5]
MAAPSPASSAARASASGLGHALVQAKQLPAHVAEALHRQASSAKTSFAAELIASGTMSAATLATASAQIFGLPLLDVTAL
DPAQLPRELLDRKICSTHTLLPLARRGKKLIVATADPSNQEAAKQIKFTTQLNVEWVVAEYDQLLQLLDASHHSTANALD
RHTGMPEEFVFENIQDMPAEEANNSSLRHEVEDAPVVKFLHKMLLDAVRMRASDLHFEPYEHHYRVRFRIDGTLREIAAP
PIAIKDKLASRIKVISRLDIAEKRVPQDGRMKLKVAANHVIDFRVSTLPTLFGEKIVIRVLDPSSAKIGIDALGYEAPEK
ARLLQAIQRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFACALRSFLRQD
PDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPSTLTRMRNMGVAPFNIASSVILITAQRLARRLCEACKAPVDL
AAHTLLEAGFTEEALDGSWRPYHAVGCAACNQGYQGRVGIYQVMPISEAMQHIILRDGSALEMAAQARAEGVRSLRESGL
HKVRWGQTSLEEVLAVTNS

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=1035052 ACA027_RS04195 WP_370681150.1 963209..964948(-) (pilB) [Comamonas sp. GB3 AK4-5]
ATGGCTGCGCCGTCTCCCGCCTCCTCTGCAGCGCGCGCTTCGGCGTCCGGACTGGGACATGCCCTGGTCCAGGCCAAGCA
GCTGCCCGCCCATGTGGCGGAAGCGCTGCACCGCCAGGCATCCAGCGCCAAGACCAGCTTTGCGGCCGAGCTGATCGCCT
CTGGAACCATGAGCGCAGCCACCCTGGCTACAGCAAGTGCACAGATTTTTGGCCTTCCGCTGCTGGATGTGACGGCACTG
GACCCGGCGCAGCTGCCGCGCGAGCTGCTGGACCGGAAAATCTGCAGCACCCACACACTGCTGCCCCTGGCCCGGCGCGG
GAAAAAGCTGATCGTGGCCACGGCAGACCCGAGCAACCAGGAGGCAGCCAAGCAGATCAAGTTCACCACCCAGCTGAATG
TGGAATGGGTGGTGGCCGAGTACGACCAGTTGCTCCAACTGCTGGACGCCAGCCACCACAGCACTGCCAATGCCCTGGAC
CGCCACACCGGCATGCCCGAGGAATTTGTCTTTGAAAACATCCAGGACATGCCCGCCGAAGAGGCAAACAACAGCAGCCT
CCGCCACGAGGTGGAAGATGCCCCGGTCGTCAAATTCCTGCACAAGATGCTGCTGGACGCCGTCCGCATGCGCGCGTCCG
ACCTGCATTTCGAGCCCTACGAGCACCACTACCGCGTGCGTTTTCGCATCGACGGCACGTTGCGTGAAATCGCCGCGCCT
CCGATTGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGGCTGGACATTGCCGAAAAACGCGTGCCTCA
GGACGGGCGCATGAAGCTCAAGGTGGCTGCCAACCACGTCATCGATTTCCGTGTCTCCACCCTGCCCACGCTGTTTGGCG
AGAAGATCGTGATCCGCGTCCTCGACCCCAGCAGCGCAAAAATAGGCATAGACGCCCTGGGCTACGAAGCCCCCGAAAAA
GCGCGCCTGCTCCAGGCCATACAGCGCCCCTACGGCATGATTTTGGTCACCGGCCCCACGGGATCGGGCAAGACGGTGTC
GCTCTACACCTGTTTGAACCTGCTGAACCAGCCCGGCGTGAACATTGCCACCGCCGAAGACCCTTCGGAAATCAATCTGC
CCGGCGTCAACCAGGTGAATGTCAACGAAAAGGCCGGACTGACCTTTGCCTGCGCGCTGCGGTCTTTTTTGCGCCAGGAT
CCGGACATCATCATGGTGGGCGAAATCCGCGACCTGGAAACGGCCGATATCGCCATCAAGGCGGCGCAAACAGGGCATCT
GGTGCTATCCACGCTGCACACCAACGATGCGCCCTCCACCCTCACGCGCATGCGCAACATGGGCGTTGCACCGTTCAACA
TCGCCTCCAGCGTGATCCTCATCACCGCCCAACGTCTGGCGCGGCGCTTGTGCGAGGCCTGCAAGGCACCGGTCGACCTA
GCTGCACACACCTTGCTGGAAGCCGGCTTTACCGAGGAGGCGCTCGACGGCTCCTGGCGGCCCTACCACGCCGTGGGCTG
CGCGGCCTGCAACCAGGGCTACCAAGGGCGCGTCGGCATCTACCAGGTCATGCCCATCAGCGAGGCCATGCAACACATCA
TCCTGCGCGACGGCAGCGCGCTGGAGATGGCCGCGCAGGCCAGAGCCGAGGGCGTACGCTCGCTGCGCGAGTCCGGCCTG
CACAAAGTCAGATGGGGCCAGACCTCGCTGGAAGAGGTGCTGGCCGTTACCAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-154)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.078

97.409

0.527
  pilF Neisseria gonorrhoeae MS11

53.147

98.791

0.525
  pilB Acinetobacter baylyi ADP1

53.723

97.409

0.523
  pilB Legionella pneumophila strain ERS1305867

50.526

98.446

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

47.183

98.1

0.463
  pilB Vibrio campbellii strain DS40M4

46.585

98.618

0.459
  pilB Vibrio cholerae strain A1552

47.662

96.028

0.458
  pilF Thermus thermophilus HB27

39.719

98.273

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.652

97.409

0.377

Multiple sequence alignment