Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QUE75_RS04150 Genome accession   NZ_AP028062
Coordinates   895272..897386 (+) Length   704 a.a.
NCBI ID   WP_286253836.1    Uniprot ID   -
Organism   Marinobacter shengliensis strain D49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 890272..902386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE75_RS04130 (MAALD49_08110) pilM 892470..893534 (+) 1065 WP_106693177.1 pilus assembly protein PilM Machinery gene
  QUE75_RS04135 (MAALD49_08120) - 893534..894097 (+) 564 WP_286253830.1 PilN domain-containing protein -
  QUE75_RS04140 (MAALD49_08130) - 894097..894708 (+) 612 WP_286253832.1 type 4a pilus biogenesis protein PilO -
  QUE75_RS04145 (MAALD49_08140) - 894711..895253 (+) 543 WP_061332384.1 pilus assembly protein PilP -
  QUE75_RS04150 (MAALD49_08150) pilQ 895272..897386 (+) 2115 WP_286253836.1 type IV pilus secretin PilQ Machinery gene
  QUE75_RS04155 (MAALD49_08160) aroK 897510..898094 (+) 585 WP_058090087.1 shikimate kinase AroK -
  QUE75_RS04160 (MAALD49_08170) aroB 898069..899157 (+) 1089 WP_286253840.1 3-dehydroquinate synthase -
  QUE75_RS04165 (MAALD49_08180) - 899201..900736 (+) 1536 WP_286253842.1 AAA family ATPase -

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75699.30 Da        Isoelectric Point: 4.5149

>NTDB_id=103460 QUE75_RS04150 WP_286253836.1 895272..897386(+) (pilQ) [Marinobacter shengliensis strain D49]
MNEKNMHEQNGLRSRLAMFKKLNVCVGVIAIGLWSSLASAVTLEDVSFSSLPGERLEVTMKFDGPPPEPTGYTIERPARI
AVDLKDTTSGLNQRSIPLGSGNAQSVTVVETKDRTRLIFNLVELTPHETVRQNNSLVMTIGGASGATATAAASQQSQSGS
TAAASSNALAGVDFRRSKEGEGRVIVDLGSASAAVNLSEQAGRIRLTMPGLDVPSDLRRRLDVTDFATPVTRIDTFVEDG
NAVVEIRPEGNYDYIAYQSGREFTVSVEKLTEEEAETRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTV
GGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPAEEIAAREKLELETSKQIAELAPVRLDIIQVNYAKAADIVA
LIRADEELISSRGFVSSDVRTNTISVRETQQKLEEIRRLVGTWDVPVRQVSIEARIVRAQTNVAESLGVRWGGAAYNVSG
NDVISIGGSLGTLQEARDAAAGGSGAISFPGALGVDMGVSGDGASSFAIGWGSDDFLIDLELSALETDGRAEVVSQPRVV
TADRQSASIKSGEEIPYQEATSSGATNVEFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNSVTTQVL
VANGETVVLGGIFQSEVATQTTKTPFLGDIPYLGRLFKRTVHIDERSELLIFITPKIIKNDLIQ

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=103460 QUE75_RS04150 WP_286253836.1 895272..897386(+) (pilQ) [Marinobacter shengliensis strain D49]
ATGAATGAAAAAAACATGCACGAACAAAATGGTTTAAGGTCGAGGCTAGCGATGTTCAAAAAACTCAATGTCTGCGTGGG
CGTGATCGCCATCGGGTTATGGAGCAGCCTGGCCAGCGCGGTCACGCTGGAAGACGTGTCGTTTTCGTCGCTGCCGGGAG
AACGCCTGGAAGTGACCATGAAATTCGATGGGCCACCGCCCGAGCCTACCGGGTACACGATTGAGCGCCCGGCGCGCATC
GCTGTGGACCTCAAGGACACCACCAGTGGGCTTAACCAGCGGAGTATTCCGCTTGGGTCCGGCAATGCGCAAAGCGTGAC
CGTGGTGGAAACCAAAGATCGCACTCGGCTGATCTTCAATCTGGTGGAATTGACTCCCCACGAGACAGTTCGTCAAAACA
ATTCGCTGGTCATGACTATTGGCGGCGCCAGTGGAGCTACGGCCACTGCGGCAGCTTCCCAGCAGAGCCAGTCAGGATCG
ACGGCCGCTGCCTCCTCCAATGCCCTTGCAGGCGTGGACTTCCGCCGGAGCAAGGAAGGCGAGGGGCGCGTCATCGTTGA
TCTTGGAAGTGCCTCTGCGGCGGTGAATCTGTCTGAGCAGGCTGGTCGCATCCGGCTGACCATGCCGGGGCTGGATGTCC
CTTCAGATCTTCGTCGTCGTCTTGATGTTACAGACTTTGCCACTCCTGTTACCCGGATCGATACATTCGTTGAGGACGGC
AACGCGGTGGTTGAGATTCGCCCGGAAGGCAATTATGACTATATTGCCTATCAGTCAGGTCGCGAGTTTACCGTTAGCGT
TGAGAAGCTTACTGAAGAAGAGGCGGAGACCCGTCGCGAAGAGAAATTCCCGTACACCGGCGACAAGCTGTCCTTGAACT
TCCAGGACATCGAAGTACGTTCGGTTCTCCAGTTGATTGCCGACTTTACCGGCCTGAACCTGGTGGCCAGTGACACCGTT
GGTGGCAGCATTACCCTGCGGTTGCAGAATGTACCCTGGGATCAGGCGCTTGACCTTATTCTGAAGACCAAGGGGCTGGA
CAAGCGTCAAATTGGCAACGTTCTGCTGGTGGCTCCGGCAGAGGAAATCGCCGCCCGCGAAAAGCTTGAGCTCGAGACCA
GTAAGCAAATCGCCGAACTGGCGCCGGTGCGCCTGGATATTATTCAGGTGAACTACGCGAAAGCGGCAGATATTGTGGCG
CTGATTCGTGCGGATGAAGAGTTGATATCCTCTCGTGGCTTTGTCTCTTCAGACGTCCGCACCAACACGATTAGCGTTCG
TGAGACGCAGCAGAAGCTGGAAGAGATTCGTCGACTTGTGGGTACCTGGGATGTACCGGTCCGTCAGGTGTCCATTGAGG
CCCGTATTGTCCGCGCCCAGACCAACGTTGCGGAGAGTCTCGGGGTTCGTTGGGGCGGCGCCGCATATAACGTCAGTGGT
AACGATGTCATCAGTATTGGTGGTTCCCTGGGTACACTCCAGGAAGCTCGTGACGCTGCGGCGGGCGGCAGTGGCGCCAT
TTCCTTCCCCGGTGCTCTGGGTGTCGATATGGGTGTTTCAGGAGACGGCGCATCATCCTTTGCGATTGGCTGGGGTAGTG
ACGACTTCCTGATCGACCTTGAGCTTTCTGCTCTTGAAACCGATGGTCGTGCCGAGGTTGTGTCTCAGCCGCGCGTGGTG
ACAGCGGATCGCCAGAGTGCCTCCATTAAGTCCGGTGAGGAAATTCCCTACCAGGAAGCAACCTCAAGCGGTGCGACAAA
CGTTGAGTTCAAGGAAGCGGTTCTGTCTCTGGAAGTAACCCCGCAGATCACTCCGGATGACAAGATCATCATGGATCTGG
TGGTAAACCAGGATTCCCGGGGTGAGGTAACAGCTGGTATTCCGTCCATCAATACCAACTCTGTTACGACTCAGGTTCTG
GTAGCCAATGGGGAGACCGTGGTGTTGGGCGGTATCTTCCAGTCTGAAGTGGCGACTCAAACCACCAAAACTCCATTCCT
GGGGGACATTCCTTACCTTGGGCGCCTGTTCAAGCGAACAGTACACATTGATGAGCGCAGCGAGTTGCTGATCTTCATCA
CACCCAAGATCATCAAAAACGATCTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(544-699)

Secretin and TonB N terminus short domain (STN)

(313-359)

Bacterial type II/III secretion system short domain (Secretin_N)

(387-449)

AMIN domain (AMIN)

(56-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.619

100

0.483
  comQ Acinetobacter baylyi ADP1

40.594

100

0.408
  pilQ Acinetobacter baumannii strain A118

39.583

100

0.405
  pilQ Acinetobacter baumannii D1279779

39.583

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

40.208

95.739

0.385
  pilQ Legionella pneumophila strain Lp02

40.059

95.739

0.384

Multiple sequence alignment