Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABK042_RS20995 Genome accession   NZ_CP166454
Coordinates   4517826..4519559 (+) Length   577 a.a.
NCBI ID   WP_005410796.1    Uniprot ID   A0AAI9G500
Organism   Stenotrophomonas maltophilia strain 1945B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4512826..4524559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK042_RS20965 (ABK042_20955) - 4512987..4513373 (+) 387 WP_049452339.1 hypothetical protein -
  ABK042_RS20970 (ABK042_20960) coaE 4513499..4514110 (-) 612 WP_049452340.1 dephospho-CoA kinase -
  ABK042_RS20975 (ABK042_20965) - 4514122..4514985 (-) 864 WP_005410800.1 A24 family peptidase -
  ABK042_RS20980 (ABK042_20970) pilC 4514993..4516252 (-) 1260 WP_049452341.1 type II secretion system F family protein Machinery gene
  ABK042_RS20985 (ABK042_20975) - 4516605..4517042 (+) 438 WP_024956771.1 pilin -
  ABK042_RS20990 (ABK042_20980) pilA/pilAI 4517195..4517602 (+) 408 WP_024956772.1 pilin Machinery gene
  ABK042_RS20995 (ABK042_20985) pilB 4517826..4519559 (+) 1734 WP_005410796.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABK042_RS21000 (ABK042_20990) pilR 4519686..4521059 (-) 1374 WP_049452342.1 sigma-54 dependent transcriptional regulator Regulator
  ABK042_RS21005 (ABK042_20995) - 4521120..4522733 (-) 1614 WP_005410794.1 PAS domain-containing sensor histidine kinase -
  ABK042_RS21010 (ABK042_21000) sucC 4523091..4524260 (+) 1170 WP_049452343.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62347.28 Da        Isoelectric Point: 5.8722

>NTDB_id=1034558 ABK042_RS20995 WP_005410796.1 4517826..4519559(+) (pilB) [Stenotrophomonas maltophilia strain 1945B]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGSDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1034558 ABK042_RS20995 WP_005410796.1 4517826..4519559(+) (pilB) [Stenotrophomonas maltophilia strain 1945B]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACCTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTTTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACTCTCGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCAGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTG
AAGCTGAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAATGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGTTGCGCTCGTTCCTGCGACAGGACCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGTCGCCTGTGCGGCAACTGCAAGCGACCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATTACCAGCTTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.872

100

0.556
  pilB Acinetobacter baumannii D1279779

55.536

98.614

0.548
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio cholerae strain A1552

48.677

98.267

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447

Multiple sequence alignment