Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB9R84_RS12010 Genome accession   NZ_CP166304
Coordinates   2499288..2501000 (+) Length   570 a.a.
NCBI ID   WP_346351576.1    Uniprot ID   -
Organism   Oceanimonas smirnovii strain OS2020-133     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494288..2506000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9R84_RS11985 (AB9R84_11975) pdhR 2495513..2496274 (-) 762 WP_019933138.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB9R84_RS11990 (AB9R84_11980) ampD 2496663..2497205 (-) 543 WP_317074450.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB9R84_RS11995 (AB9R84_11985) - 2497278..2497796 (+) 519 WP_346351578.1 retropepsin-like aspartic protease -
  AB9R84_RS12000 (AB9R84_11990) nadC 2497845..2498699 (+) 855 WP_317074448.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB9R84_RS12005 (AB9R84_11995) - 2498831..2499283 (+) 453 WP_346351577.1 pilin -
  AB9R84_RS12010 (AB9R84_12000) pilB 2499288..2501000 (+) 1713 WP_346351576.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9R84_RS12015 (AB9R84_12005) pilC 2501023..2502249 (+) 1227 WP_395535159.1 type II secretion system F family protein Machinery gene
  AB9R84_RS12020 (AB9R84_12010) pilD 2502636..2503490 (+) 855 WP_019933129.1 prepilin peptidase Machinery gene
  AB9R84_RS12025 (AB9R84_12015) coaE 2503487..2504098 (+) 612 WP_395544940.1 dephospho-CoA kinase -
  AB9R84_RS12030 (AB9R84_12020) - 2504088..2505272 (-) 1185 WP_418139391.1 CsgG/HfaB family protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62778.94 Da        Isoelectric Point: 5.3753

>NTDB_id=1034142 AB9R84_RS12010 WP_346351576.1 2499288..2501000(+) (pilB) [Oceanimonas smirnovii strain OS2020-133]
MSTFTSPSSSGLARSLVASALLSAEELELVQEQARKEGRPLGSVLVEKQWLSSEELARFCEREFMLPLSNLHDFDIKQLA
PEYLSMDLIEHHHAIPVLLEDKVLYLAMSDPTNIGALEDFSFRFNLVTDALLVEEGKLQALLQQLQQQHGAGLEFEHTLD
DDIAALEQDEQNAWQEKDRISADDDAPIVRYINNIMINAVRKEASDLHFEPYERFFRIRFRIDGILHEVASPPVQLAARL
AARLKVMAKLDIAERRLPQDGRIKLHLSGSKSVDMRVNTLPTQWGEKVVIRLLDSAATRLGIEQLGLTDVQKTLYLQALQ
RPQGMILVTGPTGSGKTVTLYTGLSILNQVTANISTAEDPIEINLPGINQVQINPKVGLSFAYALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLSHMGLPAYNIASSISLIIAQRLARKLCVHCREPEQLSPGQLSQL
GFTPAQQASGITLYRATGCDQCTDGYKGRTGVYEIMTMSEQLAELIMNNAGALAIARAAQQEGMISLRHSALAKAGQGLI
SLAEVNRITG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1034142 AB9R84_RS12010 WP_346351576.1 2499288..2501000(+) (pilB) [Oceanimonas smirnovii strain OS2020-133]
ATGAGCACCTTCACTTCTCCCTCCAGCAGCGGCCTTGCCAGAAGCCTGGTGGCTTCTGCCTTACTGTCTGCCGAAGAGCT
GGAGCTGGTACAGGAGCAGGCCAGAAAAGAAGGCAGGCCGCTGGGCTCGGTGCTGGTAGAAAAGCAGTGGCTGTCTTCCG
AGGAGCTGGCGCGCTTTTGTGAGCGTGAGTTTATGCTGCCGCTGAGCAATCTGCATGATTTCGATATCAAACAGCTTGCA
CCTGAATATCTGAGCATGGATCTGATAGAGCATCACCATGCCATTCCGGTGCTGCTGGAAGACAAAGTCCTTTACCTGGC
CATGTCTGACCCCACCAATATAGGTGCACTGGAAGACTTCAGCTTTCGCTTTAATCTGGTCACCGATGCCCTGCTGGTAG
AAGAAGGCAAGCTGCAGGCATTACTACAGCAGCTGCAGCAGCAACATGGTGCCGGGCTGGAGTTTGAACATACCCTTGAT
GATGATATTGCCGCCCTTGAACAGGATGAACAAAACGCCTGGCAGGAAAAGGACAGGATCAGCGCCGATGATGACGCGCC
CATTGTGCGCTATATCAACAACATCATGATCAATGCGGTACGCAAGGAAGCGTCCGATCTGCACTTTGAGCCCTATGAAC
GTTTTTTTCGTATTCGCTTTCGCATTGACGGCATACTGCACGAAGTGGCATCGCCGCCGGTGCAGCTGGCGGCGCGCCTG
GCTGCCCGGCTCAAGGTAATGGCAAAGCTCGATATCGCCGAGCGGCGCTTGCCTCAGGATGGCCGTATCAAACTGCACTT
GTCCGGCTCAAAATCCGTCGACATGCGAGTGAACACACTGCCCACCCAATGGGGAGAGAAGGTCGTTATTCGTCTGCTCG
ACAGCGCTGCCACCCGCCTTGGCATTGAGCAGCTGGGGTTGACTGACGTGCAGAAAACCTTATATCTGCAGGCATTACAG
CGCCCTCAGGGCATGATACTGGTCACCGGGCCCACCGGCTCGGGCAAAACCGTGACACTGTATACCGGCCTGAGTATTCT
CAATCAGGTCACTGCCAATATCTCTACTGCCGAAGACCCTATTGAAATTAATCTGCCGGGTATCAATCAGGTACAAATTA
ATCCCAAAGTCGGATTGAGCTTTGCCTATGCATTGCGGGCATTTCTACGGCAGGACCCCGATATTATTATGGTGGGCGAA
ATTCGTGATCTGGAAACCGCTGAAATTGCTGTGAAGGCAGCACAAACCGGTCATCTGGTGCTTTCTACGCTGCATACCAA
CTCGGCGGCAGAGACCCTGACCCGCCTCAGCCATATGGGGCTGCCCGCTTACAATATTGCTTCATCCATCAGCCTGATTA
TTGCCCAGCGCCTGGCCCGTAAACTCTGTGTTCATTGCCGGGAGCCGGAACAGCTGTCCCCCGGGCAACTGAGCCAGCTT
GGCTTTACGCCGGCACAGCAGGCCAGTGGCATCACACTTTATCGCGCCACAGGCTGCGACCAGTGTACAGATGGCTATAA
AGGGCGCACCGGGGTTTATGAAATCATGACCATGAGTGAGCAGCTGGCTGAGCTGATTATGAACAATGCCGGTGCCCTTG
CCATTGCACGGGCGGCACAGCAAGAGGGGATGATCTCATTACGCCACTCGGCGCTGGCCAAGGCAGGCCAGGGGCTGATC
AGTCTGGCTGAGGTAAACAGAATTACCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.332

98.772

0.507
  pilB Acinetobacter baylyi ADP1

50.174

100

0.505
  pilB Vibrio cholerae strain A1552

51.63

96.842

0.5
  pilB Acinetobacter baumannii D1279779

49.649

100

0.496
  pilB Vibrio campbellii strain DS40M4

49.027

99.123

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

48.94

99.298

0.486
  pilF Neisseria gonorrhoeae MS11

46.454

98.947

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

37.198

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.392

95.789

0.396
  pilF Thermus thermophilus HB27

37.478

98.772

0.37

Multiple sequence alignment