Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB9R83_RS11975 Genome accession   NZ_CP166302
Coordinates   2501726..2503438 (+) Length   570 a.a.
NCBI ID   WP_395544941.1    Uniprot ID   -
Organism   Oceanimonas smirnovii strain OS2020-127     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2496726..2508438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9R83_RS11950 (AB9R83_11940) pdhR 2497914..2498675 (-) 762 WP_019933138.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB9R83_RS11955 (AB9R83_11945) ampD 2499077..2499619 (-) 543 WP_395544946.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB9R83_RS11960 (AB9R83_11950) - 2499692..2500210 (+) 519 WP_395544945.1 retropepsin-like aspartic protease family protein -
  AB9R83_RS11965 (AB9R83_11955) nadC 2500259..2501113 (+) 855 WP_395544943.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB9R83_RS11970 (AB9R83_11960) - 2501242..2501724 (+) 483 WP_395544942.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB9R83_RS11975 (AB9R83_11965) pilB 2501726..2503438 (+) 1713 WP_395544941.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9R83_RS11980 (AB9R83_11970) pilC 2503461..2504687 (+) 1227 WP_395535159.1 type II secretion system F family protein Machinery gene
  AB9R83_RS11985 (AB9R83_11975) pilD 2505074..2505928 (+) 855 WP_019933129.1 prepilin peptidase Machinery gene
  AB9R83_RS11990 (AB9R83_11980) coaE 2505925..2506536 (+) 612 WP_395544940.1 dephospho-CoA kinase -
  AB9R83_RS11995 (AB9R83_11985) - 2506526..2507710 (-) 1185 WP_395544939.1 CsgG/HfaB family protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62763.93 Da        Isoelectric Point: 5.4861

>NTDB_id=1034117 AB9R83_RS11975 WP_395544941.1 2501726..2503438(+) (pilB) [Oceanimonas smirnovii strain OS2020-127]
MVTSTPHSNSGLARSLVAAALLSAEELELVQEQARKEGRPLGSVLVEKQWLSSEELARFCEREFMLPLSNLHDFDIKQIA
PEYLSMDLIEHHHAIPVLLEDKVLYLAMSDPTNIGALEDFSFRFNLVTDALLVEEGKLQALLQQLQQQHGAGLEFEHTLD
DDIAALEQDEQNAWQEKDRISADDDAPIVRYINNIMINAVRKEASDLHFEPYERFFRIRFRIDGILHEVASPPVQLAARL
AARLKVMAKLDIAERRLPQDGRIKLHLSGSKSVDMRVNTLPTQWGEKVVIRLLDSAATRLGIEQLGLTDAQKTLYLQALQ
RPQGMILVTGPTGSGKTVTLYTGLSILNQVTANISTAEDPIEINLPGINQVQINPKVGLSFAYALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLSHMGLPAYNIASSISLIIAQRLARKLCVHCREPEQLSPGQLSQL
GFTPAQQASGITLYRATGCDQCTDGYKGRTGVYEIMTMSEQLAELIMNNAGALAIARAAQQEGMISLRHSALAKAGQGLI
SLAEVNRITG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1034117 AB9R83_RS11975 WP_395544941.1 2501726..2503438(+) (pilB) [Oceanimonas smirnovii strain OS2020-127]
ATGGTTACTTCAACCCCACATTCCAATAGCGGCCTTGCCAGAAGCCTGGTGGCGGCGGCCCTGCTGTCTGCCGAAGAGCT
GGAGCTGGTACAGGAGCAGGCCAGAAAAGAAGGCCGGCCGCTGGGCTCGGTGCTGGTAGAAAAGCAGTGGCTGTCTTCCG
AGGAGCTGGCGCGCTTTTGTGAGCGTGAGTTTATGCTGCCGCTGAGCAATCTGCATGATTTCGATATCAAGCAGATTGCA
CCTGAATATCTGAGCATGGATCTGATAGAGCATCACCATGCCATTCCGGTGCTGCTGGAAGACAAAGTCCTTTACCTGGC
CATGTCTGACCCCACCAATATAGGTGCACTGGAAGACTTCAGCTTTCGCTTTAATCTGGTCACCGATGCCCTGCTGGTAG
AAGAAGGCAAGCTGCAGGCATTACTACAGCAGCTGCAGCAGCAACATGGTGCCGGGCTGGAGTTTGAACATACCCTTGAT
GATGATATTGCCGCCCTTGAACAGGATGAACAAAACGCCTGGCAGGAAAAGGACAGGATCAGCGCCGATGATGACGCGCC
CATTGTGCGCTATATCAACAACATCATGATCAATGCGGTACGCAAGGAAGCGTCCGATCTGCACTTTGAGCCCTATGAAC
GTTTTTTTCGTATTCGCTTTCGCATTGACGGCATACTGCACGAAGTGGCATCGCCGCCGGTGCAGCTGGCGGCCCGCCTG
GCTGCCCGGCTCAAGGTAATGGCAAAGCTCGATATCGCCGAGCGGCGCTTGCCTCAGGATGGCCGTATCAAGCTGCACTT
GTCCGGCTCAAAATCCGTCGACATGCGAGTGAACACACTGCCCACCCAATGGGGAGAAAAGGTCGTCATTCGTCTGCTCG
ACAGCGCTGCCACCCGCCTTGGCATTGAGCAGCTGGGGCTGACTGACGCGCAGAAAACCTTATATCTGCAGGCATTACAG
CGCCCTCAGGGCATGATACTGGTCACCGGGCCCACCGGCTCGGGCAAAACCGTGACACTGTATACCGGCCTGAGTATTCT
CAATCAGGTCACTGCCAATATTTCTACTGCCGAAGACCCTATTGAAATTAATCTGCCGGGTATCAATCAGGTACAAATTA
ATCCCAAAGTCGGATTGAGCTTTGCCTATGCATTGCGGGCATTTCTACGGCAGGACCCCGATATTATTATGGTGGGCGAA
ATTCGTGATCTGGAAACCGCTGAAATTGCTGTGAAGGCAGCACAAACCGGTCATCTGGTGCTTTCTACGCTGCATACCAA
CTCGGCGGCAGAGACCCTGACCCGCCTCAGCCATATGGGGCTGCCCGCTTACAATATTGCTTCATCCATCAGCCTGATTA
TTGCCCAGCGCCTGGCCCGTAAACTCTGTGTTCATTGCCGGGAGCCGGAACAGCTGTCCCCCGGGCAACTGAGCCAGCTT
GGCTTTACGCCGGCACAGCAGGCCAGTGGCATCACACTTTATCGCGCCACAGGCTGCGACCAGTGTACAGATGGCTATAA
AGGGCGCACCGGGGTTTATGAAATCATGACCATGAGTGAGCAGCTGGCTGAGCTGATTATGAACAATGCCGGTGCCCTTG
CCATTGCACGGGCGGCACAGCAGGAGGGGATGATCTCATTACGCCACTCGGCGCTGGCCAAGGCAGGCCAGGGGCTGATC
AGTCTGGCTGAGGTAAACAGAATTACCGGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.332

98.772

0.507
  pilB Vibrio cholerae strain A1552

51.444

97.193

0.5
  pilB Acinetobacter baylyi ADP1

49.912

100

0.5
  pilB Acinetobacter baumannii D1279779

49.735

99.123

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

48.858

99.825

0.488
  pilB Vibrio campbellii strain DS40M4

49.027

99.123

0.486
  pilF Neisseria gonorrhoeae MS11

46.441

98.596

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

37.56

100

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.392

95.789

0.396
  pilF Thermus thermophilus HB27

37.634

97.895

0.368

Multiple sequence alignment