Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8813_RS17340 Genome accession   NZ_CP166095
Coordinates   4017315..4019060 (-) Length   581 a.a.
NCBI ID   WP_104613413.1    Uniprot ID   -
Organism   Xanthomonas arboricola pv. corylina strain NCPPB 935     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4012315..4024060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8813_RS17325 (AB8813_17395) sucC 4012499..4013668 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  AB8813_RS17330 (AB8813_17400) - 4013900..4015513 (+) 1614 WP_039512337.1 PAS domain-containing sensor histidine kinase -
  AB8813_RS17335 (AB8813_17405) pilR 4015706..4017100 (+) 1395 WP_104613414.1 sigma-54 dependent transcriptional regulator Regulator
  AB8813_RS17340 (AB8813_17410) pilB 4017315..4019060 (-) 1746 WP_104613413.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8813_RS17345 (AB8813_17415) - 4019092..4019568 (-) 477 WP_343237020.1 pilin -
  AB8813_RS17350 (AB8813_17420) - 4019643..4020083 (-) 441 WP_104613411.1 pilin -
  AB8813_RS17355 (AB8813_17425) pilC 4020430..4021686 (+) 1257 WP_104613429.1 type II secretion system F family protein Machinery gene
  AB8813_RS17360 (AB8813_17430) - 4021693..4022556 (+) 864 WP_016849610.1 A24 family peptidase -
  AB8813_RS17365 (AB8813_17435) coaE 4022570..4023193 (+) 624 WP_104613410.1 dephospho-CoA kinase -
  AB8813_RS17370 (AB8813_22510) - 4023203..4023441 (+) 239 Protein_3399 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62988.08 Da        Isoelectric Point: 5.7615

>NTDB_id=1033342 AB8813_RS17340 WP_104613413.1 4017315..4019060(-) (pilB) [Xanthomonas arboricola pv. corylina strain NCPPB 935]
MNAGPATNNINLVGITGIARRLVQDGALDEVSARSAMHQAANAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVF
DASQNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGS
SLGDDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVA
KAPVKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEAD
QQKLFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLR
QDPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKS
TLPEHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQS
ALVKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=1033342 AB8813_RS17340 WP_104613413.1 4017315..4019060(-) (pilB) [Xanthomonas arboricola pv. corylina strain NCPPB 935]
ATGAATGCTGGGCCTGCCACTAACAACATTAATCTCGTAGGTATAACCGGCATTGCCCGGCGATTGGTCCAAGACGGTGC
TCTAGACGAAGTCAGTGCTCGCTCCGCCATGCATCAGGCAGCCAACGCAAAAGTCCCTTTGCCGCAATGGTTCGCTGAAA
AAAAGTTGGTGTCGGCAGCGCAACTCGCCTCTGCCAATGCGGTTGAGTTTGGTATGCCCCTGATGGACGTTTCTGTCTTT
GATGCCAGCCAGAACGCAGTCAAGCTAGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGG
CAATCGGTTGTTCGTGGGGGTGAGCAACCCGACCCAGACTCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGG
TCGAGCCGATCCTGGTCGACGAGGATCAGATCCGTCGCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCT
TCGCTCGGTGACGACGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTC
CGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAG
CCTCCGACATCCATTTCGAGCCCTATGAAGACGACTATCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAATGTGGCC
AAGGCACCGGTGAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGT
GCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACTTTGCCGACGTTGT
TCGGCGAGAAGGTGGTGCTGCGTATCCTTGACGGCAGCGCGGCCAAGCTGGGAATCGAGAAGCTGGGCTACGAGGCGGAC
CAGCAGAAGCTGTTCCTGGAGGCAATCCATAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGAC
GGTGTCGCTGTATACGGCACTGGGTATCCTCAACGACGAGACGCGCAACATCTCCACGGCCGAGGACCCGGTGGAAATCC
GTCTGCCCGGCGTGAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGT
CAGGACCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGG
TCACATGGTGCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCGT
ACAACATCACCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCG
ACCTTGCCCGAGCATGCGTTGCTGGCGGAAGGGTTCACTGCAGAGCAGATCGCTGCCGGGATCGAGCTGTACGAAGCGGT
CGGTTGCGACGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCG
CGATCGTGCTGGAAGGCGGTAACGCCATGCAGATTGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCG
GCGTTGGTCAAGGCCGCGCACGGGGTGACCAGCCTGGCCGAGATCAACCGGGTGACGAAAGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-156)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

97.762

0.546
  pilB Acinetobacter baylyi ADP1

55.458

97.762

0.542
  pilB Legionella pneumophila strain ERS1305867

52.373

97.935

0.513
  pilB Vibrio cholerae strain A1552

48.527

99.312

0.482
  pilF Neisseria gonorrhoeae MS11

48.239

97.762

0.472
  pilB Vibrio campbellii strain DS40M4

45.694

97.935

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

46.25

96.386

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

35.961

100

0.377

Multiple sequence alignment