Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8S08_RS01840 Genome accession   NZ_CP165718
Coordinates   390349..392046 (-) Length   565 a.a.
NCBI ID   WP_153826419.1    Uniprot ID   A0AB39XAF8
Organism   Pseudidiomarina sp. PP-1MA     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 385349..397046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8S08_RS01815 (AB8S08_01815) mutT 386323..386721 (+) 399 WP_369743234.1 8-oxo-dGTP diphosphatase MutT -
  AB8S08_RS01820 (AB8S08_01820) zapD 386767..387531 (-) 765 WP_369743235.1 cell division protein ZapD -
  AB8S08_RS01825 (AB8S08_01825) coaE 387563..388186 (-) 624 WP_369743237.1 dephospho-CoA kinase -
  AB8S08_RS01830 (AB8S08_01830) - 388187..389071 (-) 885 WP_369743239.1 A24 family peptidase -
  AB8S08_RS01835 (AB8S08_01835) pilC 389072..390298 (-) 1227 WP_369743240.1 type II secretion system F family protein Machinery gene
  AB8S08_RS01840 (AB8S08_01840) pilB 390349..392046 (-) 1698 WP_153826419.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8S08_RS01845 (AB8S08_01845) - 392134..392625 (-) 492 WP_369743241.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB8S08_RS01850 (AB8S08_01850) nadC 393016..393882 (-) 867 WP_369743242.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB8S08_RS01855 (AB8S08_01855) ampD 394059..394610 (+) 552 WP_369744172.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB8S08_RS01860 (AB8S08_01860) ampE 394685..395467 (+) 783 WP_369743243.1 regulatory signaling modulator protein AmpE -
  AB8S08_RS01865 (AB8S08_01865) pdhR 395553..396308 (+) 756 WP_110075934.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62725.91 Da        Isoelectric Point: 5.6726

>NTDB_id=1031320 AB8S08_RS01840 WP_153826419.1 390349..392046(-) (pilB) [Pseudidiomarina sp. PP-1MA]
MLHHSLAALLLRDKLIPEDKLTRCSEYAWENKLSFTACLMEQKLLSASALANFCYLESNAPWLDLDAFDPDFIPQKFINE
RLIRKNHAVPLYQRGNVLYIAVSDPTRIETLDEFQFRTRLQTEAVLVEENKLQRLIDKLLENEHSALGVTEQDEQELRDI
DAADERQDDSGAVSADVKNDAPIVIYINKMLLDAIKRGASDLHFEPYETEYRVRFRIDGVLHEIARPPVALAGRIAARLK
VMAQLNIAERRLPQDGRIKLRLSKHKSIDFRVSSLPTLYGEKIVLRILDAAAAMIGIESLGYEPDQKALYEEALSKPQGM
ILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLNGINQVHINAKAGLNFADALRSFLRQDPDVVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAAETLTRLMNMGVPSYNIAGSVTLIIAQRLARRLCNSCKAEEHLPQDELLRQGFKAE
QLADLTIYKAVGCEHCTGGYKGRTGVYEVMPITPAIQDLIMEGGSSLDIAKAARDDGIHSLRESGLLKVAKGIISLAEAN
RVTNF

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1031320 AB8S08_RS01840 WP_153826419.1 390349..392046(-) (pilB) [Pseudidiomarina sp. PP-1MA]
ATGCTGCACCACTCCTTAGCGGCTCTGTTGCTGCGTGACAAGCTGATACCAGAAGATAAGCTAACGCGTTGCAGTGAGTA
TGCGTGGGAGAACAAGCTGTCGTTTACGGCGTGCTTGATGGAGCAGAAACTCCTGTCTGCAAGTGCACTGGCCAATTTTT
GCTATCTAGAAAGCAATGCTCCGTGGCTAGACCTTGATGCCTTTGATCCTGACTTTATTCCCCAGAAATTTATCAATGAA
CGTTTGATTCGCAAAAACCATGCGGTGCCTTTGTATCAGCGTGGTAATGTCCTCTATATTGCCGTGTCGGACCCTACTCG
AATTGAGACGCTAGATGAGTTTCAATTTCGTACGCGGTTGCAAACCGAGGCGGTGTTGGTTGAGGAGAACAAGCTCCAGC
GTTTAATTGATAAGTTGCTTGAGAACGAGCATTCGGCCCTAGGCGTCACCGAGCAAGATGAACAAGAGCTGCGCGATATT
GATGCCGCTGATGAGCGCCAAGATGATTCGGGTGCTGTCTCTGCTGATGTCAAAAACGATGCTCCTATTGTCATCTACAT
CAATAAGATGTTGCTAGATGCGATTAAGCGTGGTGCCTCGGATTTGCACTTTGAGCCTTATGAAACCGAGTATCGGGTGC
GCTTTCGAATCGATGGCGTGCTGCATGAAATTGCGCGCCCTCCGGTGGCTTTAGCGGGGCGCATTGCGGCACGCCTAAAA
GTCATGGCCCAGCTGAATATCGCTGAGCGGCGCTTGCCACAAGACGGTCGGATTAAGCTGCGCTTATCCAAACATAAGTC
CATTGATTTTCGGGTAAGTAGCTTGCCTACCCTGTATGGCGAAAAAATTGTACTGCGGATACTTGATGCCGCCGCCGCCA
TGATTGGGATTGAATCGCTGGGCTACGAACCAGACCAAAAAGCCTTGTACGAAGAAGCACTCAGTAAGCCACAAGGGATG
ATTTTGGTAACCGGCCCCACCGGCTCAGGTAAAACCGTAAGTTTGTACACCGGCTTGAATATTCTCAATACGCCCGAACG
TAATATTTCTACCGCCGAAGACCCCGTCGAAATCAACTTAAATGGTATTAATCAGGTACATATTAACGCTAAAGCGGGCT
TAAACTTTGCTGATGCGTTGCGTTCATTTTTGCGCCAAGACCCGGACGTGGTGATGGTGGGTGAGATCCGTGACTTAGAA
ACCGCGGAAATTGCGATTAAGGCGGCACAAACCGGGCACTTAGTTTTGTCCACGTTGCACACCAACTCGGCGGCAGAAAC
CCTGACCCGTTTGATGAACATGGGCGTGCCCTCTTACAACATTGCCGGCTCGGTCACCCTGATTATTGCGCAGCGGCTAG
CACGACGCTTGTGTAACTCCTGCAAAGCGGAAGAGCACTTACCCCAAGATGAATTATTACGACAGGGCTTTAAAGCCGAG
CAGTTAGCTGATTTGACCATCTATAAAGCCGTGGGCTGCGAGCATTGCACCGGCGGTTACAAAGGTCGGACCGGGGTGTA
TGAAGTGATGCCCATTACTCCGGCTATTCAAGATTTGATCATGGAAGGCGGTTCGTCTTTAGATATCGCCAAAGCCGCTC
GTGATGATGGCATTCATTCACTGCGTGAGTCGGGTTTACTTAAAGTAGCCAAAGGCATTATTAGTTTAGCCGAGGCAAAT
CGCGTCACTAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.782

98.938

0.572
  pilB Acinetobacter baumannii D1279779

56.912

98.584

0.561
  pilB Legionella pneumophila strain ERS1305867

55.929

100

0.559
  pilB Vibrio parahaemolyticus RIMD 2210633

49.387

100

0.499
  pilB Vibrio cholerae strain A1552

54.118

90.265

0.488
  pilB Vibrio campbellii strain DS40M4

54.472

87.08

0.474
  pilF Neisseria gonorrhoeae MS11

49.245

93.805

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

37.363

96.637

0.361
  pilF Thermus thermophilus HB27

38.636

93.451

0.361

Multiple sequence alignment