Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB5B06_RS10330 Genome accession   NZ_CP163419
Coordinates   1203156..1204910 (+) Length   584 a.a.
NCBI ID   WP_416049736.1    Uniprot ID   -
Organism   Cupriavidus basilensis strain BL-MT-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1198156..1209910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5B06_RS10295 proB 1198167..1199285 (-) 1119 WP_309403639.1 glutamate 5-kinase -
  AB5B06_RS10300 obgE 1199325..1200425 (-) 1101 WP_416049735.1 GTPase ObgE -
  AB5B06_RS10305 - 1200415..1200597 (+) 183 WP_193692090.1 hypothetical protein -
  AB5B06_RS10310 rpmA 1200575..1200835 (-) 261 WP_006162509.1 50S ribosomal protein L27 -
  AB5B06_RS10315 rplU 1200906..1201217 (-) 312 WP_006162510.1 50S ribosomal protein L21 -
  AB5B06_RS10320 ispB 1201790..1202719 (+) 930 WP_416050387.1 octaprenyl diphosphate synthase -
  AB5B06_RS10330 pilF 1203156..1204910 (+) 1755 WP_416049736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB5B06_RS10335 pilC 1204941..1206206 (+) 1266 WP_416049737.1 type II secretion system F family protein Machinery gene
  AB5B06_RS10340 pilD 1206210..1207127 (+) 918 WP_416049738.1 prepilin peptidase Machinery gene
  AB5B06_RS10345 coaE 1207188..1207799 (+) 612 WP_416049739.1 dephospho-CoA kinase -
  AB5B06_RS10350 zapD 1208074..1208832 (+) 759 WP_150986821.1 cell division protein ZapD -
  AB5B06_RS10355 - 1208837..1209022 (+) 186 WP_043349767.1 DNA gyrase inhibitor YacG -
  AB5B06_RS10360 - 1209062..1209502 (-) 441 WP_183037048.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 64196.78 Da        Isoelectric Point: 7.0953

>NTDB_id=1029597 AB5B06_RS10330 WP_416049736.1 1203156..1204910(+) (pilF) [Cupriavidus basilensis strain BL-MT-10]
MTLGLALAQSRRIAPALLAQLEQSAREKKSQLIDEIIASGTMTAHDVAIFAADKYQLPLIDLNQYNLNKVPPALAGNREF
HAHRLLPLGRRENRLVLALSDPGNQAGLDAIKQKYNLPVEPVVVEHDKLMQYVRSASDDSGGAIKSIGPGHVERKMIDYD
PVASAGALRNKTAADIDDAPVVRFLHKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPVDIRDKISTRIKVL
SRLDISEKRVPQDGRMKLLIALPKEKGGDKDGKDSKEPKETVEKAIDFRVSTLPTLFGEKIVIRILESSSDKLDIDQLGY
EPAQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNEKAGLTFSAALRS
FLRQDPDIIMVGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTCKR
PGELAREVLADAGFQDEDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEPMQQIILAHGTALEIAEQARKDGVLSL
REAGLAKVKQGLTSLEEVLATTNM

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=1029597 AB5B06_RS10330 WP_416049736.1 1203156..1204910(+) (pilF) [Cupriavidus basilensis strain BL-MT-10]
ATGACACTTGGTCTAGCTCTTGCACAGAGCCGGCGTATCGCGCCGGCTCTGCTCGCCCAGCTTGAACAGTCGGCACGAGA
AAAAAAGTCGCAGCTGATCGACGAGATCATCGCCAGCGGCACCATGACTGCACATGATGTGGCGATCTTCGCGGCTGATA
AGTATCAGCTGCCGCTGATCGATCTGAATCAGTACAACCTCAACAAGGTGCCGCCGGCGCTGGCAGGCAATCGCGAGTTC
CACGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAGAACCGCCTGGTGCTGGCGTTGTCCGACCCGGGCAACCAGGCCGG
GCTGGACGCCATCAAGCAGAAATACAACCTGCCGGTGGAACCCGTCGTGGTCGAGCATGACAAGCTGATGCAATACGTGC
GCTCGGCTTCCGACGACAGCGGCGGCGCGATCAAGTCGATCGGTCCTGGCCATGTCGAGCGCAAGATGATCGACTACGAC
CCGGTGGCATCCGCCGGGGCGCTGCGCAACAAGACCGCGGCCGACATCGACGATGCGCCCGTGGTTCGCTTCCTGCACAA
GCTGCTGACGGAAGCCTTCCATCGCGGCGCCTCCGATCTTCACTTCGAGCCGTTCGAGACCTTCTACCGCATCCGCTTCC
GTGTGGATGGCGTGCTGCAGGAAGTGGCACGGCCGCCGGTGGACATCCGCGACAAGATCTCCACCCGGATCAAGGTGTTG
TCGCGCCTGGACATCTCGGAAAAGCGCGTGCCGCAAGATGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGAAAAGGG
CGGCGACAAAGACGGCAAGGACAGCAAGGAGCCCAAGGAAACCGTGGAGAAGGCGATCGACTTCCGCGTCTCCACGCTGC
CGACGCTGTTCGGCGAGAAGATCGTGATCCGGATCCTGGAGTCGTCGTCCGACAAGCTCGACATCGACCAGCTCGGCTAC
GAGCCGGCGCAAAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAG
CGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCGGCTGAACCAGGGCGACATCAATATCTCGACCGCCGAGGATCCGG
CGGAAATCCAATTGCCCGGCATCAACCAGGTCAACGTCAACGAAAAAGCCGGCCTGACCTTCTCCGCCGCGCTGCGCTCC
TTCCTGCGCCAGGATCCCGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCGGC
ACAGACTGGCCACCTAGTGTTCTCGACGCTGCATACCAACGACGCGCCGACCACGCTCACGCGCCTGATGAACATGGGTG
TCGCGCCCTTCAATATCGCGTCGAGCGTGCTGATGATCACCGCGCAGCGGCTTGCGCGGCGGCTGTGCACCTGCAAGCGC
CCCGGCGAGCTGGCGCGCGAGGTGCTGGCCGACGCCGGCTTCCAGGACGAAGACCTCGACGGCAGCTGGCAGCCTTACCA
CCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTGATGCCGATCACTGAGC
CGATGCAGCAGATCATCCTCGCGCATGGCACGGCGCTTGAGATCGCCGAACAAGCGCGTAAAGATGGCGTGCTATCGTTG
CGCGAAGCTGGCCTGGCCAAGGTGAAGCAGGGTCTCACCTCGCTCGAAGAAGTGCTGGCCACAACAAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-260)

Type II/IV secretion system protein (T2SSE)

(281-474)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.169

100

0.509
  pilB Acinetobacter baumannii D1279779

48.403

100

0.493
  pilB Acinetobacter baylyi ADP1

49.037

97.774

0.479
  pilB Legionella pneumophila strain ERS1305867

47.183

97.26

0.459
  pilB Vibrio cholerae strain A1552

43.911

99.829

0.438
  pilB Vibrio parahaemolyticus RIMD 2210633

45.324

95.205

0.432
  pilB Vibrio campbellii strain DS40M4

44.665

94.692

0.423
  pilF Thermus thermophilus HB27

37.5

100

0.375

Multiple sequence alignment