Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4L27_RS06585 Genome accession   NZ_CP162907
Coordinates   1432158..1433843 (+) Length   561 a.a.
NCBI ID   WP_104969782.1    Uniprot ID   -
Organism   Vibrio diabolicus strain 3098     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427158..1438843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4L27_RS06565 (AB4L27_06565) pdhR 1428738..1429505 (-) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB4L27_RS06570 (AB4L27_06570) ampD 1429911..1430462 (-) 552 WP_005396682.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB4L27_RS06575 (AB4L27_06575) nadC 1430555..1431442 (+) 888 WP_104969780.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB4L27_RS06580 (AB4L27_06580) pilA 1431705..1432154 (+) 450 WP_104969781.1 pilin Machinery gene
  AB4L27_RS06585 (AB4L27_06585) pilB 1432158..1433843 (+) 1686 WP_104969782.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4L27_RS06590 (AB4L27_06590) pilC 1433867..1435090 (+) 1224 WP_104969783.1 type II secretion system F family protein Machinery gene
  AB4L27_RS06595 (AB4L27_06595) pilD 1435155..1436024 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  AB4L27_RS06600 (AB4L27_06600) coaE 1436025..1436639 (+) 615 WP_047008979.1 dephospho-CoA kinase -
  AB4L27_RS06605 (AB4L27_06605) zapD 1436667..1437407 (+) 741 WP_012841416.1 cell division protein ZapD -
  AB4L27_RS06610 (AB4L27_06610) yacG 1437555..1437749 (+) 195 WP_005396692.1 DNA gyrase inhibitor YacG -
  AB4L27_RS06615 (AB4L27_06615) rplS 1438409..1438762 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62171.99 Da        Isoelectric Point: 5.4010

>NTDB_id=1027961 AB4L27_RS06585 WP_104969782.1 1432158..1433843(+) (pilB) [Vibrio diabolicus strain 3098]
MVTNLSTVLRQSGLLSLAQEESLLEQVKASGISMPEALLSSGFFTSSELVEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTTSTLLLAVADPTNQQAEDDFRFATGLQIELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMNTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1027961 AB4L27_RS06585 WP_104969782.1 1432158..1433843(+) (pilB) [Vibrio diabolicus strain 3098]
TTGGTTACCAACCTCTCAACGGTTCTTCGTCAATCCGGGTTACTTAGCTTAGCTCAAGAAGAATCGTTACTTGAGCAAGT
CAAAGCTTCTGGCATTTCAATGCCGGAAGCTTTACTCAGTTCAGGATTCTTCACGTCAAGTGAACTGGTAGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTACGCCTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACACGGCATAACGCACTCCCACTCCAGCGTACGACTTCAACGTTATTACTCGCCGTTGCCGACCCGACGAACCA
ACAAGCAGAAGACGATTTCCGCTTTGCCACTGGTTTACAGATTGAATTGGTGTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAATTAGCCAAC
CTGGTTGATGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAATCTCCTGTTAGTCGTTATATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGTTGCGATGGCATCTTAATCGAAACACAGCAGCCGCCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGATTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAACACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

94.652

100

0.947
  pilB Vibrio campbellii strain DS40M4

89.84

100

0.898
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Acinetobacter baumannii D1279779

49.639

98.752

0.49
  pilB Legionella pneumophila strain ERS1305867

47.979

100

0.487
  pilB Acinetobacter baylyi ADP1

47.576

99.287

0.472
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

37.086

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.018

100

0.376
  pilF Thermus thermophilus HB27

36.121

100

0.362

Multiple sequence alignment