Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4P91_RS03305 Genome accession   NZ_CP162590
Coordinates   767881..769956 (-) Length   691 a.a.
NCBI ID   WP_150687610.1    Uniprot ID   -
Organism   Pseudomonas sp. B21128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 762881..774956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P91_RS03290 (AB4P91_03280) - 764509..766095 (-) 1587 WP_395604310.1 AAA family ATPase -
  AB4P91_RS03295 (AB4P91_03285) aroB 766106..767206 (-) 1101 WP_395604311.1 3-dehydroquinate synthase -
  AB4P91_RS03300 (AB4P91_03290) aroK 767358..767876 (-) 519 WP_042557267.1 shikimate kinase AroK -
  AB4P91_RS03305 (AB4P91_03295) pilQ 767881..769956 (-) 2076 WP_150687610.1 type IV pilus secretin PilQ Machinery gene
  AB4P91_RS03310 (AB4P91_03300) - 769970..770497 (-) 528 WP_395604312.1 pilus assembly protein PilP -
  AB4P91_RS03315 (AB4P91_03305) pilO 770494..771117 (-) 624 WP_150593941.1 type 4a pilus biogenesis protein PilO -
  AB4P91_RS03320 (AB4P91_03310) - 771114..771680 (-) 567 WP_150593940.1 PilN domain-containing protein -
  AB4P91_RS03325 (AB4P91_03315) pilM 771680..772744 (-) 1065 WP_150593939.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74921.39 Da        Isoelectric Point: 5.5789

>NTDB_id=1027275 AB4P91_RS03305 WP_150687610.1 767881..769956(-) (pilQ) [Pseudomonas sp. B21128]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYDTRVEGNYLFVVVGQGAPAKAPRPAVAAPRAATTAAPAKAFVPKSRAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAGVTGDRTVITVEPSGT
FDYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVNG
SSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=1027275 AB4P91_RS03305 WP_150687610.1 767881..769956(-) (pilQ) [Pseudomonas sp. B21128]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGTTGTCACCGATGGTACAGGCGGCCAACTTGAA
GGCGCTGGATGTGGCGGCGTTGCCGGGTGATCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCGCAGCCCAAGG
GTTACACCACCGAGTCACCGGCGCGAATCGCGCTGGACTTGCCCGGTGTGGCCAGTCAACTGGCGAGCAAAAATCTCGAT
CTGGGCAGTGGCAATGCGCGCACGGCCACGGTGGTTGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTCACGCAATT
GGCGCCTTACGACACTCGGGTTGAGGGCAACTATTTGTTCGTGGTGGTTGGTCAGGGCGCGCCGGCCAAGGCGCCGCGAC
CGGCAGTGGCGGCACCACGCGCGGCGACTACTGCTGCACCCGCCAAGGCGTTCGTACCGAAATCCCGCGCCATTCGCGGT
GTGGATTTTCAACGCGGCACGGCGGGTGAGGGCAATGTCGTCATCGACCTGTCCGACCCGACCATCGCCCCGGATATCCA
GGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTACCGGAAAAGCTGCGAGTGCGTCTCGACGTCAAGG
ATTTCGCCACGCCGGTGCAATTCGTCAACGCCGGCGTAACCGGTGATCGCACGGTCATTACCGTCGAGCCCAGCGGCACC
TTTGACTATTCCACGTTCCAGACCGACAACAAGCTGACCGTCAGCATTCGCCCGATGACCGTCGACGACCTGCAAAAACG
CAATGCCGATCGTCAGGCCTATGTCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATTGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCGAGCGACACGGTGCAGGGCGGCATTACCCTGCGTTTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGCCTGGATAAACGCAAAATCGGTAATGTGCTGCTGGTGGC
CCCGGCTGATGAAATTGCCGCGCGCGAACGTCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGCTGCGTC
GTGAGCTATTGCAGGTGAATTACGCCAAGGCTGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCCGAGGCCAAG
ATCGACGAGCGTGGATCAATCACTGTCGACGAACGGACCAACAACATCATTGCCTACCAGACTCAGGATCGTCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCG
ATTACGACAAGAGTCTGGGGGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAATGCTTCCGGGGTCAACGGT
TCATCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCATTTGTCGACATGGGGACTGTCGGCAATACTTC
CGGGATCGGTATCGCGTTCATTACCGACAACGTTCTGCTCGATCTGGAGCTGACGGCCATGGAGAAGACCGGCAACGGTG
AAATCGTTTCGCAGCCCAAAGTGGTCACCTCCGACAAAGAGACCGCGAAGATCCTCAAAGGCACCGAGATTCCTTACCAG
GAAGCCAGCTCAAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACCCCGCAGATCACCCC
GGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGATTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAAAAGAACGAGGTCAACGCGAAAGTGCTCGTCAATGATGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAATACT
CAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.867

100

0.754
  comQ Acinetobacter baylyi ADP1

41.126

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.358

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.358

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.829

0.373
  pilQ Legionella pneumophila strain Lp02

38.166

97.829

0.373

Multiple sequence alignment