Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB4P91_RS03325 Genome accession   NZ_CP162590
Coordinates   771680..772744 (-) Length   354 a.a.
NCBI ID   WP_150593939.1    Uniprot ID   -
Organism   Pseudomonas sp. B21128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 766680..777744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P91_RS03300 (AB4P91_03290) aroK 767358..767876 (-) 519 WP_042557267.1 shikimate kinase AroK -
  AB4P91_RS03305 (AB4P91_03295) pilQ 767881..769956 (-) 2076 WP_150687610.1 type IV pilus secretin PilQ Machinery gene
  AB4P91_RS03310 (AB4P91_03300) - 769970..770497 (-) 528 WP_395604312.1 pilus assembly protein PilP -
  AB4P91_RS03315 (AB4P91_03305) pilO 770494..771117 (-) 624 WP_150593941.1 type 4a pilus biogenesis protein PilO -
  AB4P91_RS03320 (AB4P91_03310) - 771114..771680 (-) 567 WP_150593940.1 PilN domain-containing protein -
  AB4P91_RS03325 (AB4P91_03315) pilM 771680..772744 (-) 1065 WP_150593939.1 pilus assembly protein PilM Machinery gene
  AB4P91_RS03330 (AB4P91_03320) - 772961..775408 (+) 2448 WP_150593938.1 penicillin-binding protein 1A -
  AB4P91_RS03335 (AB4P91_03325) - 775748..777016 (-) 1269 WP_042557260.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38105.63 Da        Isoelectric Point: 4.7417

>NTDB_id=1027276 AB4P91_RS03325 WP_150593939.1 771680..772744(-) (pilM) [Pseudomonas sp. B21128]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLAKARTGLRSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVGEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1027276 AB4P91_RS03325 WP_150593939.1 771680..772744(-) (pilM) [Pseudomonas sp. B21128]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGTCGTCAGGGTGATCGCTACCGGGTCGAGGCTTATGCGGTAGAGCCGCTGCCACCGAACGCCGTGGTCGAAAAAAACA
TCGCCGAGCTCGAGGGCGTGGGCCAGGCCCTGACCCGGGTGCTGGCCAAGGCACGCACCGGGCTGAGAAGCGTGGCGGTG
GCGGTGGCCGGTTCAGCGGTGATTACCAAGATCATCGAGATGGACGCCGGGCTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATTGAAGCCGACCAGTACATTCCCTATCCGCTCGACGAAGTCGCCATCGACTTCGAGGTTCAGGGCGTGTCGCCGC
GCAACCCTGAACGGGTCAACGTGTTGCTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCACTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAAGCGTACGCGCTGGAGCGTTCGTTCGGCTTGCTCGCGACCCAGCTGGC
GGCTTCTCAAGAGCGCTTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAGTTGTTCGGCGGTCGGCAATTGACCGAGGAGATCCAGCGTCGCTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCAAAGAAGCAGGGCGGTCTGCCCGACGATTACGTCGGCGAGGTGTTGCAGCCGTTTCGCGAGGC
GCTGGTGCAGCAGGTATCGCGTTCGTTGCAGTTTTTCTTCGCTTCCGGCCAGTACAACGCGGTCGATCATATTCTGCTGG
CTGGCGGCACGGCGTCGGTGCCGGGGCTGGACCGGCTGATCGAACAGCGCCTGGATACTCCGACCCAAGTGGCCAATCCG
TTTTCCAATATGGCCCTGAGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment