Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4P97_RS26165 Genome accession   NZ_CP162582
Coordinates   5608608..5610683 (+) Length   691 a.a.
NCBI ID   WP_395601799.1    Uniprot ID   -
Organism   Pseudomonas sp. A1230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5603608..5615683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P97_RS26145 (AB4P97_26075) pilM 5605820..5606884 (+) 1065 WP_115145994.1 pilus assembly protein PilM Machinery gene
  AB4P97_RS26150 (AB4P97_26080) - 5606884..5607450 (+) 567 WP_042557263.1 PilN domain-containing protein -
  AB4P97_RS26155 (AB4P97_26085) pilO 5607447..5608070 (+) 624 WP_042557264.1 type 4a pilus biogenesis protein PilO -
  AB4P97_RS26160 (AB4P97_26090) - 5608067..5608594 (+) 528 WP_047299398.1 pilus assembly protein PilP -
  AB4P97_RS26165 (AB4P97_26095) pilQ 5608608..5610683 (+) 2076 WP_395601799.1 type IV pilus secretin PilQ Machinery gene
  AB4P97_RS26170 (AB4P97_26100) aroK 5610688..5611206 (+) 519 WP_042557267.1 shikimate kinase AroK -
  AB4P97_RS26175 (AB4P97_26105) aroB 5611358..5612458 (+) 1101 WP_150687609.1 3-dehydroquinate synthase -
  AB4P97_RS26180 (AB4P97_26110) - 5612469..5614061 (+) 1593 WP_395601800.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74850.27 Da        Isoelectric Point: 5.4106

>NTDB_id=1027198 AB4P97_RS26165 WP_395601799.1 5608608..5610683(+) (pilQ) [Pseudomonas sp. A1230]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYDTRVEGNYLFVVVGQGAPARAPRPAVAASSAATPAAPAKAFVAKPRAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAGVTGDRTVITVEPSGT
FDYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVNG
SSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=1027198 AB4P97_RS26165 WP_395601799.1 5608608..5610683(+) (pilQ) [Pseudomonas sp. A1230]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGTTGTCACCGATGGTACAGGCGGCCAATTTGAA
GGCGCTGGATGTGGCAGCGTTGCCGGGTGATCGCGTAGAACTGAAGCTGTCGTTCGACGGCCCGCCGCCGCAGCCCAAGG
GTTACACCACCGAATCACCGGCGCGAATTGCGCTGGACTTGCCCGGTGTGGCCAGTCAGTTGGCGAGCAAAAATCTCGAT
CTGGGCAGTGGCAATGCGCGCACGGCCACGGTGGTTGAAGCCAAGGACCGCACGCGGCTGATTGTCAGTCTCACGCAATT
GGCGCCTTACGACACACGGGTTGAGGGCAACTATCTGTTCGTGGTGGTTGGTCAGGGCGCGCCGGCCAGGGCGCCGCGAC
CGGCAGTGGCGGCATCAAGCGCGGCGACTCCTGCTGCGCCCGCCAAGGCGTTCGTGGCGAAACCCCGCGCCATTCGCGGT
GTGGATTTTCAGCGCGGTACTGCGGGCGAGGGCAATGTCGTCATCGACCTGTCCGACCCGACCATCGCCCCGGATATCCA
GGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAATTGCCGGAAAAGCTGCGGGTGCGTCTCGACGTGAAGG
ATTTCGCCACGCCAGTGCAATTCGTCAACGCCGGCGTAACCGGTGATCGCACGGTCATTACCGTCGAGCCCAGCGGCACC
TTTGACTATTCCACGTTCCAGACCGATAACAAACTGACCGTCAGCATCCGTCCGATGACCGTCGACGACCTGCAAAAACG
CAACGCCGATCGTCAGGCCTATGTCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATTGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCGAGCGACACGGTGCAGGGCGGCATCACCCTGCGTTTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTGAAGACCAAAGGCCTGGATAAACGCAAAATCGGTAATGTGCTGCTGGTGGC
CCCGGCTGATGAAATTGCTGCGCGCGAACGTCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGCTGCGTC
GTGAGCTGTTGCAGGTGAACTATGCCAAGGCTGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGCGCCGAGGCCAAG
ATCGACGAGCGTGGATCAATCACTGTCGACGAACGGACCAACAACATCATTGCCTACCAGACTCAGGATCGTCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCG
ATTACGACAAGAGTCTGGGGGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAATGCTTCCGGGGTCAACGGT
TCATCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGGACCGTCGGCAATACTTC
CGGGATCGGTATCGCGTTCATTACCGACAACGTTTTGCTCGATCTGGAGCTGACGGCCATGGAGAAGACCGGCAACGGTG
AAATCGTTTCGCAGCCCAAAGTGGTCACCTCCGACAAAGAGACCGCGAAGATCCTCAAAGGCACCGAGATTCCTTACCAG
GAAGCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACCCCGCAGATCACCCC
GGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAAAAGAACGAGGTCAACGCCAAAGTGCTGGTCAATGATGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAATACT
CAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.361

100

0.754
  pilQ Acinetobacter baumannii D1279779

38.493

100

0.414
  pilQ Acinetobacter baumannii strain A118

38.493

100

0.414
  comQ Acinetobacter baylyi ADP1

41.27

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.829

0.373
  pilQ Legionella pneumophila strain Lp02

38.166

97.829

0.373

Multiple sequence alignment