Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB4P97_RS26145 Genome accession   NZ_CP162582
Coordinates   5605820..5606884 (+) Length   354 a.a.
NCBI ID   WP_115145994.1    Uniprot ID   -
Organism   Pseudomonas sp. A1230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5600820..5611884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P97_RS26135 (AB4P97_26065) - 5601540..5602808 (+) 1269 WP_042557260.1 malic enzyme-like NAD(P)-binding protein -
  AB4P97_RS26140 (AB4P97_26070) - 5603156..5605603 (-) 2448 WP_261736244.1 penicillin-binding protein 1A -
  AB4P97_RS26145 (AB4P97_26075) pilM 5605820..5606884 (+) 1065 WP_115145994.1 pilus assembly protein PilM Machinery gene
  AB4P97_RS26150 (AB4P97_26080) - 5606884..5607450 (+) 567 WP_042557263.1 PilN domain-containing protein -
  AB4P97_RS26155 (AB4P97_26085) pilO 5607447..5608070 (+) 624 WP_042557264.1 type 4a pilus biogenesis protein PilO -
  AB4P97_RS26160 (AB4P97_26090) - 5608067..5608594 (+) 528 WP_047299398.1 pilus assembly protein PilP -
  AB4P97_RS26165 (AB4P97_26095) pilQ 5608608..5610683 (+) 2076 WP_395601799.1 type IV pilus secretin PilQ Machinery gene
  AB4P97_RS26170 (AB4P97_26100) aroK 5610688..5611206 (+) 519 WP_042557267.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38091.60 Da        Isoelectric Point: 4.7417

>NTDB_id=1027197 AB4P97_RS26145 WP_115145994.1 5605820..5606884(+) (pilM) [Pseudomonas sp. A1230]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGQALTRVLGKARTGLRSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1027197 AB4P97_RS26145 WP_115145994.1 5605820..5606884(+) (pilM) [Pseudomonas sp. A1230]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCCGTGAAGCTGCTGGAACT
TAGTCGTCAGGGTGATCGCTACCGGGTCGAGGCTTATGCGGTGGAGCCGCTGCCACCGAACGCCGTGGTCGAGAAAAACA
TCGCCGAGCTCGAGGGTGTGGGCCAGGCCTTGACCCGGGTGCTGGGCAAGGCACGCACCGGGCTGAGAAGCGTGGCGGTG
GCGGTGGCCGGTTCAGCGGTGATTACCAAGATCATCGAGATGGACGCCGGGCTTTCCGACGACGAACTGGAAAACCAGCT
CAAGATTGAAGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTCGAGGTTCAGGGCGTGTCGCCGC
GCAACCCCGAGCGGGTCAACGTGTTGCTGGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCACTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAAGCGTACGCGCTGGAGCGTTCGTTCGGCTTGCTCGCCACGCAGCTGGC
GGCTTCTCAAGAGCGCTTGACCGTGGCTGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGTGAGCAGTTGTTCGGCGGCCGGCAACTGACCGAGGAGATCCAGCGTCGCTATGGCCTGACCGTC
GAGCAGGCGGGGCTGGCAAAGAAGCAGGGCGGTCTGCCCGACGATTACGTCAGCGAGGTGTTGCAGCCGTTTCGCGAGGC
GCTGGTGCAGCAGGTATCGCGTTCGTTGCAGTTTTTCTTCGCTTCCGGCCAGTACAACGCGGTCGACCATATTCTGCTCG
CTGGCGGCACGGCGTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGCCTGGATACTCCGACCCAAGTGGCCAATCCG
TTTGCCAATATGGCCCTGAGCAGCAAGGTCAACGCCGGGGCGCTGGCCAGTGACGCGCCGGCCTTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment