Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB4P92_RS20440 Genome accession   NZ_CP162581
Coordinates   4412736..4414436 (-) Length   566 a.a.
NCBI ID   WP_395598064.1    Uniprot ID   -
Organism   Pseudomonas sp. A0308     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4407736..4419436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P92_RS20410 (AB4P92_20380) - 4408849..4409538 (+) 690 WP_395598061.1 energy-coupling factor ABC transporter permease -
  AB4P92_RS20415 (AB4P92_20385) - 4409604..4409819 (+) 216 WP_042561116.1 hypothetical protein -
  AB4P92_RS20420 (AB4P92_20390) yacG 4409825..4410025 (-) 201 WP_042561115.1 DNA gyrase inhibitor YacG -
  AB4P92_RS20425 (AB4P92_20395) coaE 4410022..4410645 (-) 624 WP_150597110.1 dephospho-CoA kinase -
  AB4P92_RS20430 (AB4P92_20400) pilD 4410642..4411514 (-) 873 WP_395598062.1 prepilin peptidase Machinery gene
  AB4P92_RS20435 (AB4P92_20405) pilC 4411516..4412733 (-) 1218 WP_395598063.1 type II secretion system F family protein Machinery gene
  AB4P92_RS20440 (AB4P92_20410) pilB 4412736..4414436 (-) 1701 WP_395598064.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB4P92_RS20445 (AB4P92_20415) - 4414663..4415094 (+) 432 WP_042561110.1 pilin -
  AB4P92_RS20450 (AB4P92_20420) - 4415394..4415750 (+) 357 WP_042561109.1 BON domain-containing protein -
  AB4P92_RS20455 (AB4P92_20425) - 4416016..4416315 (-) 300 WP_395598065.1 DUF2845 domain-containing protein -
  AB4P92_RS20460 (AB4P92_20430) - 4416312..4419263 (-) 2952 WP_395599685.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62652.48 Da        Isoelectric Point: 5.5460

>NTDB_id=1027162 AB4P92_RS20440 WP_395598064.1 4412736..4414436(-) (pilB) [Pseudomonas sp. A0308]
MNDIALSGLAKQLVQAELLTEESAQQAWQQAQRNRLSLVSYLVQNKLVKSAQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHGTGLEDMADVDLDG
LDVESIDDNKQDAIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKRAIEIPRETLIKEGFPE
ERIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1027162 AB4P92_RS20440 WP_395598064.1 4412736..4414436(-) (pilB) [Pseudomonas sp. A0308]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTCACGGAAGAAAGTGCCCAGCAGGC
CTGGCAACAGGCCCAGCGCAATCGGCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTAAAGAGCGCACAGGTCG
CCGAGATCGCCTCCGAGCACTTCGGCATGGCGTTCCTCGACCTTAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCATGCCCTGCCCTTGTGGCGGCGCGGCAACAAGCTGTTCGTGGGAATTTCCGA
CCCGAGCAATCATCAGGCAATCAACGACATCCAGTTCAGCACCGGGTTGAGCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAAAAATTCTTCGACACTCACGGCACCGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
CTCGATGTCGAATCGATCGACGACAACAAGCAGGACGCCATCGGCGGACAAGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCCTCCGACCTGCATTTCGAGCCTTACGAAAAAAACTACCGGG
TACGGGTGCGCACCGACGGCATTCTGCGCGAGGTGGCCAAGCCGCCGATCCAACTGGCCGGGCGCATCGCCGCACGTTTG
AAAGTCATGGCCAGCCTCGACATCTCCGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCCAAAAGCAA
GTCGATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTCGATCCCTCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTATGAGCCTGAGCAGAAAGACCTGTATTTGGCAGCCCTCAAGCAGCCACAAGGG
ATGATTCTGGTCACCGGCCCCACTGGCTCGGGCAAAACGGTGTCGCTGTACACCGGGCTGAATATTCTCAACACGGTCGA
CATCAACATATCCACCGCCGAAGACCCGGTGGAGATCAATATGGAAGGCATCAACCAGGTCAACGTCAACCCCAGGCAGG
GGCTCGACTTTGCCCAGGCCTTGCGCTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGCCACCTCGTGCTTTCGACACTGCACACCAACAGCGCCGCGGA
AACCCTGACGCGACTGCATAACATGGGCATCCCCGGCTTCAACATCGCCACTTCGGTCAGCCTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAGCCACTGCAAGAGAGCCATCGAGATTCCGCGCGAAACCTTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTACGAGCCAGTCGGTTGCGATCACTGCAATGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAGAACACAGCAGACCTGCAACGACTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGTACCTCAGGCCTGCGCAAGGCCATGCAAGGCATTACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.092

99.647

0.569
  pilB Legionella pneumophila strain ERS1305867

55.575

99.823

0.555
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.296

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.868

93.64

0.364
  pilF Thermus thermophilus HB27

36.559

98.587

0.36

Multiple sequence alignment