Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB3464_RS18790 Genome accession   NZ_CP162519
Coordinates   4196852..4198927 (+) Length   691 a.a.
NCBI ID   WP_397457782.1    Uniprot ID   -
Organism   Pseudomonas asplenii strain UPB 526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4191852..4203927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3464_RS18770 (AB3464_18680) pilM 4194066..4195130 (+) 1065 WP_397457779.1 pilus assembly protein PilM Machinery gene
  AB3464_RS18775 (AB3464_18685) - 4195130..4195696 (+) 567 WP_397457780.1 PilN domain-containing protein -
  AB3464_RS18780 (AB3464_18690) pilO 4195693..4196316 (+) 624 WP_010453643.1 type 4a pilus biogenesis protein PilO -
  AB3464_RS18785 (AB3464_18695) pilP 4196313..4196840 (+) 528 WP_397457781.1 type 4a pilus biogenesis lipoprotein PilP -
  AB3464_RS18790 (AB3464_18700) pilQ 4196852..4198927 (+) 2076 WP_397457782.1 type IV pilus secretin PilQ Machinery gene
  AB3464_RS18795 (AB3464_18705) aroK 4198932..4199450 (+) 519 WP_010453648.1 shikimate kinase AroK -
  AB3464_RS18800 (AB3464_18710) aroB 4199600..4200700 (+) 1101 WP_397457783.1 3-dehydroquinate synthase -
  AB3464_RS18805 (AB3464_18715) - 4200711..4202294 (+) 1584 WP_010453652.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74769.99 Da        Isoelectric Point: 5.4228

>NTDB_id=1026851 AB3464_RS18790 WP_397457782.1 4196852..4198927(+) (pilQ) [Pseudomonas asplenii strain UPB 526]
MNRILSALGISLWMALVSPMVFAANLKTLDVAALPGDRVEIKLAFDSPPAAPHGYTTEQPARIALDLTGVASQLATKTRD
LGVGNARSVTVVEAKDRTRLIINLTKLAPYSTRIEGNNLFVVVGQGAAPAVATPVASTRPVATAAQPARVAPKAYVPVGK
AIRSVDFQRGEQGEGNVVIELSDPSISPDVQEKGGKISVEFPRTQLPESLRVRLDVKDFATPVQYVNATASADRASISIE
PSGAFDYSTYQTDNKLTISVRPLTVDDLQRRNAERFVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AESKGDERGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKQLGARWGGSISGGNWKTNG
GSPTLSRLTGQSASTPFVDLGATSATSGIGIGFVTDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGTEIPYQ
ESSSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYQNVLNNVPPIKKNEVNAKVLVNDGETVVIGGVFSNT
QSKTEEKVPFLGDVPYVGRLFRRDIVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=1026851 AB3464_RS18790 WP_397457782.1 4196852..4198927(+) (pilQ) [Pseudomonas asplenii strain UPB 526]
ATGAACAGGATCCTTTCCGCCCTCGGCATTTCGCTATGGATGGCGCTGGTGTCGCCGATGGTGTTCGCGGCCAATCTCAA
GACCCTCGACGTGGCGGCACTGCCGGGCGATCGGGTCGAGATCAAGCTGGCTTTCGACAGCCCGCCTGCGGCCCCGCACG
GCTATACCACCGAGCAGCCGGCGCGGATTGCGCTGGACCTGACCGGAGTGGCCAGCCAACTGGCTACCAAGACTCGCGAC
CTGGGTGTCGGTAACGCGCGCAGCGTAACCGTGGTGGAGGCCAAGGATCGGACCCGGCTGATCATCAACCTGACCAAGCT
GGCGCCCTACAGCACGCGGATCGAGGGCAACAACCTGTTCGTGGTGGTCGGTCAGGGCGCTGCACCCGCTGTTGCTACTC
CGGTCGCCAGTACCCGGCCGGTAGCTACCGCTGCGCAGCCGGCGCGTGTGGCGCCCAAAGCCTACGTGCCTGTTGGCAAG
GCCATTCGCAGCGTTGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAATGTGGTGATCGAGCTTTCCGATCCCTCCATCAG
TCCGGATGTGCAGGAAAAGGGCGGCAAGATCAGCGTGGAGTTTCCGCGTACCCAACTGCCGGAATCCCTGCGCGTGCGCC
TGGATGTGAAGGATTTCGCCACGCCGGTGCAATACGTCAACGCCACGGCGTCTGCCGACCGGGCAAGCATCTCCATCGAG
CCGAGTGGTGCCTTCGATTATTCGACCTACCAGACCGACAATAAACTGACCATCAGTGTCCGTCCGCTGACCGTCGATGA
CCTGCAGCGACGTAACGCCGAGCGCTTTGTCTACACCGGTGAAAAACTCTCGCTGAACTTCCAGGACATCGATGTGCGTT
CGGTTCTGCAACTGATCGCCGATTTTACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAAGGCGGGATCACCCTGCGC
CTGCAAAACGTGCCGTGGGACCAGGCATTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAACGT
GCTGTTGGTGGCGCCGGCGGACGAGATCGCTGCGCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCAGAGCTTG
CGCCACTGCGTCGTGAGCTGCTGCAGGTCAACTACGCCAAGGCCGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGC
GCCGAGAGCAAGGGCGACGAGCGTGGCTCGATCACCGTGGACGAACGCACCAACAACATCATTGCCTACCAGACCCAGGA
GCGTCTCGACGAACTGCGACGGATCGTTGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGTATCGTCG
AGGCCAACGTCGGTTATGACAAACAATTGGGGGCCCGTTGGGGCGGCAGTATCTCGGGTGGCAACTGGAAAACCAATGGC
GGCTCGCCAACGTTGTCGAGGTTGACTGGCCAGAGTGCCAGTACACCGTTCGTCGATCTGGGAGCAACCTCGGCGACCTC
CGGCATCGGTATCGGCTTCGTCACCGACAACACCATCCTCGACCTGGAACTGAGTGCGATGGAGAAGACCGGTAACGGTG
AGGTCGTGTCGCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATCCCGTACCAG
GAGTCCAGCTCCAGCGGTGCCACTTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTTGAGGTCACGCCGCAGATCACCCC
GGACAATCGGATCATCATGGAGGTGAAGGTCACCAAGGATGAGCCGGACTACCAGAACGTGCTCAACAACGTACCGCCGA
TCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTCAATGACGGTGAAACCGTGGTGATTGGCGGTGTGTTCTCCAATACT
CAGAGCAAAACCGAAGAGAAGGTGCCATTTCTCGGCGATGTGCCGTATGTTGGGCGCCTTTTCCGTCGGGACATCGTTTC
GGAAGCAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.338

100

0.771
  comQ Acinetobacter baylyi ADP1

42.157

100

0.436
  pilQ Acinetobacter baumannii strain A118

39.136

100

0.42
  pilQ Acinetobacter baumannii D1279779

39.136

100

0.42
  pilQ Legionella pneumophila strain Lp02

39.201

97.829

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.201

97.829

0.384

Multiple sequence alignment