Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB3464_RS18770 Genome accession   NZ_CP162519
Coordinates   4194066..4195130 (+) Length   354 a.a.
NCBI ID   WP_397457779.1    Uniprot ID   -
Organism   Pseudomonas asplenii strain UPB 526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4189066..4200130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3464_RS18760 (AB3464_18670) - 4189964..4191232 (+) 1269 WP_010453637.1 malic enzyme-like NAD(P)-binding protein -
  AB3464_RS18765 (AB3464_18675) - 4191428..4193860 (-) 2433 WP_397459831.1 penicillin-binding protein 1A -
  AB3464_RS18770 (AB3464_18680) pilM 4194066..4195130 (+) 1065 WP_397457779.1 pilus assembly protein PilM Machinery gene
  AB3464_RS18775 (AB3464_18685) - 4195130..4195696 (+) 567 WP_397457780.1 PilN domain-containing protein -
  AB3464_RS18780 (AB3464_18690) pilO 4195693..4196316 (+) 624 WP_010453643.1 type 4a pilus biogenesis protein PilO -
  AB3464_RS18785 (AB3464_18695) pilP 4196313..4196840 (+) 528 WP_397457781.1 type 4a pilus biogenesis lipoprotein PilP -
  AB3464_RS18790 (AB3464_18700) pilQ 4196852..4198927 (+) 2076 WP_397457782.1 type IV pilus secretin PilQ Machinery gene
  AB3464_RS18795 (AB3464_18705) aroK 4198932..4199450 (+) 519 WP_010453648.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38187.56 Da        Isoelectric Point: 4.5877

>NTDB_id=1026850 AB3464_RS18770 WP_397457779.1 4194066..4195130(+) (pilM) [Pseudomonas asplenii strain UPB 526]
MLGLFGKKAHSLLGIDISSTSVKLLELSRSGNRYRVESYAVEPLPPNAVIEKNIAELEGVGQALTRVLLKARTSAREAAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALGL
AGLDARVVDVEAYALERSFGLLAAQLATDHDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVMEVLQPFKDALVQQVSRSLQFFFASGQYNDVDYILLAGGTASIAGLDHLIQQRLGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1026850 AB3464_RS18770 WP_397457779.1 4194066..4195130(+) (pilM) [Pseudomonas asplenii strain UPB 526]
GTGCTTGGACTCTTCGGCAAAAAAGCGCATTCGCTCCTGGGCATAGACATCAGCTCCACCTCCGTGAAACTCCTCGAATT
GAGCCGCTCAGGCAATCGATATCGGGTCGAGTCCTACGCGGTGGAGCCGCTGCCGCCCAATGCCGTGATCGAGAAAAACA
TCGCCGAACTCGAGGGGGTTGGCCAGGCCCTGACGCGCGTGCTGCTAAAGGCCCGTACCAGTGCGCGCGAAGCCGCGGTG
GCGGTGGCCGGTTCAGCGGTCATTACCAAGACCATCGAGATGGATGCCGGGCTCTCCGACGATGATATGGAGAACCAGCT
CAAGATAGAGGCCGACCAGTACATTCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGGTATTCGGCGC
GCAATCCCGAGCGGGTCGAAGTGCTCTTGGCCGCCTGTCGGAAAGAAAACGTCGAAGTGCGCGAAGCAGCCCTTGGCCTG
GCCGGACTCGATGCCCGCGTAGTCGATGTCGAAGCGTATGCGCTGGAGCGCTCCTTTGGCCTGCTGGCTGCCCAGTTGGC
TACCGATCACGACAGGCTGACGGTCGCTGTGGTCGATATCGGCGCCACCATGACCACCCTGAGTGTCCTGCATAACGGAC
GGATCATCTATACCCGCGAACAGTTGTTCGGTGGACGTCAGCTCACCGAGGAAATCCAGCGGCGCTATGGCCTCTCCAAT
GAAGAGGCCGGATTGGCGAAGAAGCAGGGCGGTTTGCCCGACGACTATGTCATGGAGGTCCTGCAGCCATTCAAGGATGC
CCTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGATGTCGACTACATCCTGCTGG
CCGGCGGTACCGCTTCGATTGCCGGCCTCGATCACCTGATCCAGCAGCGCCTGGGTACGCCGACCATGGTCGCCAACCCG
TTCGCCGACATGGCATTGAGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCGCCAGCCCTGATGATCGCCTGTGG
TCTGGCGTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

47.042

100

0.472

Multiple sequence alignment