Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABZP26_RS14225 Genome accession   NZ_CP162514
Coordinates   3048129..3049805 (+) Length   558 a.a.
NCBI ID   WP_064664023.1    Uniprot ID   A0AB39APA1
Organism   Pseudoalteromonas sp. SD03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3043129..3054805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP26_RS14205 (ABZP26_14205) pilA/pilAI 3044245..3044679 (+) 435 WP_368485085.1 pilin Machinery gene
  ABZP26_RS14210 (ABZP26_14210) pilC 3044793..3046019 (+) 1227 WP_368485086.1 type II secretion system F family protein Machinery gene
  ABZP26_RS14215 (ABZP26_14215) pilD 3046451..3047362 (+) 912 WP_036956689.1 A24 family peptidase Machinery gene
  ABZP26_RS14220 (ABZP26_14220) coaE 3047365..3047994 (+) 630 WP_036956690.1 dephospho-CoA kinase -
  ABZP26_RS14225 (ABZP26_14225) pilB 3048129..3049805 (+) 1677 WP_064664023.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZP26_RS14230 (ABZP26_14230) yacG 3049870..3050100 (+) 231 WP_008114915.1 DNA gyrase inhibitor YacG -
  ABZP26_RS14235 (ABZP26_14235) - 3050160..3050648 (-) 489 WP_368485087.1 Spy/CpxP family protein refolding chaperone -
  ABZP26_RS14240 (ABZP26_14240) - 3050816..3051505 (+) 690 WP_016899750.1 response regulator transcription factor -
  ABZP26_RS14245 (ABZP26_14245) - 3051483..3052910 (+) 1428 WP_368485088.1 ATP-binding protein -
  ABZP26_RS14250 (ABZP26_14250) - 3052900..3053844 (+) 945 WP_024601725.1 alpha/beta fold hydrolase -
  ABZP26_RS14255 (ABZP26_14255) - 3053841..3054671 (-) 831 WP_368485623.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62388.67 Da        Isoelectric Point: 6.1023

>NTDB_id=1026725 ABZP26_RS14225 WP_064664023.1 3048129..3049805(+) (pilB) [Pseudoalteromonas sp. SD03]
MNINSPLLRKFITLGRVDTDTVKTRQQDFASTAELISKCGKIDSKDLAEQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYVQLDNKIEQLLDATGSLNLSEDEFKEFAELDSEQ
HKEHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1026725 ABZP26_RS14225 WP_064664023.1 3048129..3049805(+) (pilB) [Pseudoalteromonas sp. SD03]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACTGATACCGTAAAAACACGGCAGCA
AGATTTTGCGTCAACTGCAGAGCTTATCTCTAAATGTGGAAAAATAGACAGCAAAGATTTAGCTGAGCAATGCATTGATT
TATTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTCGCACAAATATCAGCTGAGCTGGTTAAAGAAAAACTGATC
CGCAAACACCATATTTTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCAGATCCTACCGATTATGGTGC
CTTCGAAAACTTTGAATTTAGCACGGGTTTATCGTGCGAAGTAGTTGTGGTTGACTACGTCCAGCTCGATAACAAAATTG
AGCAATTACTCGATGCCACTGGCAGTTTAAATTTAAGTGAAGACGAATTTAAAGAGTTCGCTGAACTCGATTCTGAGCAG
CACAAGGAACACACACCTAAAGACGATGACAAAGACGACGCCCCAATAATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGCGCCTCAGATTTACACTTTGAACCTTATGAACACAAATACCGTATTCGCTTTCGCATTGATGGTATTT
TGCACGAAGTAGCCAGCCCGCCAAATACATTGGCCAGTAAGCTATCGGCGCGAATAAAAGTAATGTCGCGACTGGATATT
GCAGAAAAGCGTAAACCACAAGATGGCCGTATTAAGCTTAAGGTAACAGAGCGTAAAAGCATTGATTTTCGTGTTAGTAC
CATGCCAACTTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCCAGTGCCATGCTAGGTATTGATGTATTAG
GCTATGAACCCGAGCAGAAAAAACTATACCTCGATGCACTTGAGCAACCGCAAGGAATGATTTTGGTTACCGGCCCAACC
GGTTCAGGTAAAACGGTTTCTTTGTATACGGGCTTAAATATACTCAATCAGCCGGAGCGGAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATACTAAAGCAGATATGACCTTTGCTAATGCACTGC
GGGCGTTTTTACGTCAAGATCCCGATGTGGTGATGGTGGGTGAAATACGTGATTTAGAAACTGCAGAAATATCAATTAAA
GCGGCGCAAACAGGTCACTTGGTGCTGTCAACACTGCATACCAACTCAGCGCCAGAAACGATTACTCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTGGCCAGTTCTATTAGTTTAATTATAGCTCAGCGTTTAGCCCGAAGATTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCTCGCGAAGAGCTTATACGCCAAGGCTTTAGTGAACAACAAATTCAAGACATGGTTTTA
TACGCACCTAAAGGCTGTGAAAACTGTACCGATGGCTACAAAGGCCGTGTTGGTATTTACGAAGTAATGAAAATTACGCC
TGAAATTGCCCAAATTATAATGCGCGGCGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGATATAACAATT
TGCGTTTATCGGGTATTCGTAAAGCTGCAGAGGGTGTGACCTCACTTGCTGAGATTAATCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.593

96.774

0.557
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

55.078

91.756

0.505
  pilB Vibrio cholerae strain A1552

52.06

95.699

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.685

95.699

0.495
  pilB Vibrio campbellii strain DS40M4

51.124

95.699

0.489
  pilF Neisseria gonorrhoeae MS11

49.808

93.548

0.466
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.382

92.832

0.366

Multiple sequence alignment