Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB3W23_RS26025 Genome accession   NZ_CP162336
Coordinates   5543671..5545371 (+) Length   566 a.a.
NCBI ID   WP_034075677.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 0303d1.1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5538671..5550371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3W23_RS26005 (AB3W23_26005) - 5539384..5541639 (-) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  AB3W23_RS26010 (AB3W23_26010) nadC 5541925..5542773 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB3W23_RS26020 (AB3W23_26020) - 5542977..5543441 (-) 465 WP_031630343.1 pilin -
  AB3W23_RS26025 (AB3W23_26025) pilB 5543671..5545371 (+) 1701 WP_034075677.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB3W23_RS26030 (AB3W23_26030) pilC 5545375..5546595 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  AB3W23_RS26035 (AB3W23_26035) pilD 5546599..5547471 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AB3W23_RS26040 (AB3W23_26040) coaE 5547468..5548079 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  AB3W23_RS26045 (AB3W23_26045) yacG 5548076..5548276 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  AB3W23_RS26050 (AB3W23_26050) - 5548313..5548522 (-) 210 WP_003094660.1 hypothetical protein -
  AB3W23_RS26055 (AB3W23_26055) - 5548628..5549317 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  AB3W23_RS26060 (AB3W23_26060) - 5549314..5549784 (-) 471 WP_003094664.1 hypothetical protein -
  AB3W23_RS26065 (AB3W23_26065) - 5549781..5550206 (-) 426 WP_003094666.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62414.30 Da        Isoelectric Point: 5.5814

>NTDB_id=1024946 AB3W23_RS26025 WP_034075677.1 5543671..5545371(+) (pilB) [Pseudomonas aeruginosa strain 0303d1.1]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
AISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQN
MILVTGPTGSGKTVSLYTGLNILNTTDINISTVEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1024946 AB3W23_RS26025 WP_034075677.1 5543671..5545371(+) (pilB) [Pseudomonas aeruginosa strain 0303d1.1]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GCCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAATCACCAAGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCCATGGACGCTCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTGGGCAAGGCAGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCAAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCCGCGCTCAAACAGCCACAGAAC
ATGATTCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGTCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.261

100

0.564
  pilB Legionella pneumophila strain ERS1305867

54.291

100

0.548
  pilB Vibrio cholerae strain A1552

51.083

97.88

0.5
  pilF Neisseria gonorrhoeae MS11

49.823

99.647

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

52.247

94.346

0.493
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.439

100

0.408
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.909

97.173

0.378

Multiple sequence alignment