Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABVF20_RS08615 Genome accession   NZ_CP160999
Coordinates   1981020..1982699 (-) Length   559 a.a.
NCBI ID   WP_267392008.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1976020..1987699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVF20_RS08585 (ABVF20_09465) - 1976350..1977267 (-) 918 WP_100156465.1 ABC transporter ATP-binding protein -
  ABVF20_RS08590 (ABVF20_09470) yacG 1977317..1977514 (-) 198 WP_267392005.1 DNA gyrase inhibitor YacG -
  ABVF20_RS08595 (ABVF20_09475) zapD 1977514..1978266 (-) 753 WP_100140881.1 cell division protein ZapD -
  ABVF20_RS08600 (ABVF20_09480) coaE 1978272..1978886 (-) 615 WP_267392006.1 dephospho-CoA kinase -
  ABVF20_RS08605 (ABVF20_09485) pilD 1978883..1979734 (-) 852 WP_367417839.1 A24 family peptidase Machinery gene
  ABVF20_RS08610 (ABVF20_09490) pilG 1979741..1980973 (-) 1233 WP_100156468.1 type II secretion system F family protein Machinery gene
  ABVF20_RS08615 (ABVF20_09495) pilF 1981020..1982699 (-) 1680 WP_267392008.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABVF20_RS08620 (ABVF20_09500) recJ 1982899..1984596 (-) 1698 WP_267392009.1 single-stranded-DNA-specific exonuclease RecJ -
  ABVF20_RS08625 (ABVF20_09505) - 1984593..1985567 (-) 975 WP_367577644.1 hypothetical protein -
  ABVF20_RS08630 (ABVF20_09510) - 1985616..1987382 (-) 1767 WP_370389591.1 M61 family metallopeptidase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62245.86 Da        Isoelectric Point: 6.1433

>NTDB_id=1022266 ABVF20_RS08615 WP_267392008.1 1981020..1982699(-) (pilF) [Snodgrassella alvi strain Acer07]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPMLLQSGVIDADKLATFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILENLGQESTSLLEGMDTVGDAEATGNN
AMSADDEDGENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPISDEMQRIIMNNGNEVDISKQAHKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1022266 ABVF20_RS08615 WP_267392008.1 1981020..1982699(-) (pilF) [Snodgrassella alvi strain Acer07]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATGCTGTTGCAGTCTGGTGTCATCGATGCTGACAAACTGGCCACCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTTCCCATTTTCAAACGTGGCAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATCCTGAATTTGGTTATTGTACGCGATGATCAGCTGCGTCAGATAC
TAGAGAATCTCGGACAGGAATCCACCAGCCTACTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAGGACGGTGAAAACGGACCTGTGGCCAGATTTGTCTTCAAAGTATTGAATGATGCACTCAA
CAGCGGCGCCTCAGATATTCACTTTGAATTTTACGAAGAAATGGCAAGAGTACGTTTTCGTATTGACGGGCAGCTACGGG
AAGTAGTGCGTATGCCACTGAATATTAGAAACCAGATTGCCTCCCGTATTAAAGTGATGGCTAAACTGGACATTTCAGAA
AAACGTATACCTCAGGATGGCCGAATACAGATTCAATTCCGCAAAGGTGCCAAACCGATTGATTTTCGTGTCAGCACCCT
TCCGACCCTGTTTGGTGAAAAAATTGTGATGCGTATTTTGAATTCGGATGCTGCTACTCTGAATATCGAACAGCTCGGCT
TCGAACCATTTCAGAAAGAATTACTGCTGGAGGCCATTCACCGGCCGTATGGTATGGTACTGGTTACTGGCCCAACCGGT
TCCGGTAAAACTGTATCCCTTTACACCTGCCTGAATATTCTGAATACCGACAGTGTGAACATTTCTACTGCAGAAGATCC
TGCGGAAATTAATTTACCCGGTATCAATCAGGTAAATGTAAATGATAAACAGGGACTGACATTTGCCGCAGCCCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGGGATTTAGAAACGGCCGATATTGCCATCAAAGCA
GCACAAACCGGTCATATGGTATTTTCTACCCTGCACACCAATAATGCGCCTGCCACCTTATCACGTATGCTGAATATGGG
GGTTGCTCCATTCAACATCGCAAGTTCAGTGAATCTGATTATGGCACAGCGTCTGGTTCGACGTTTATGCACCTGCAAAG
CACCAGCCGAACGGCCACCAGAATTCGCACTCAAAAAAGCCGGTTTCTCTGATGAAGATCTAGCTAAAGAATGGACATTT
TATCGGCCGGTAGGCTGTGAGCGCTGTAAAGGCAAAGGTTACAAGGGGCGCGCTGGTGTTTATGAAGTAATGCCTATTAG
TGATGAAATGCAGCGAATCATCATGAACAATGGTAATGAAGTCGATATCAGTAAGCAGGCGCATAAAGAAGGACTGGTCG
ATTTGCGTCATGCTGGTCTGCTTAAAGTTATGGAAGGCATCACTTCGCTTGACGAAATTCTAGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.929

100

0.691
  pilB Acinetobacter baylyi ADP1

50.528

100

0.513
  pilB Acinetobacter baumannii D1279779

51.589

95.707

0.494
  pilB Legionella pneumophila strain ERS1305867

48.33

100

0.492
  pilB Vibrio cholerae strain A1552

45.583

100

0.462
  pilB Vibrio campbellii strain DS40M4

44.014

100

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

43.44

100

0.438
  pilF Thermus thermophilus HB27

40.335

96.243

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.226

95.17

0.383

Multiple sequence alignment