Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB2N10_RS10630 Genome accession   NZ_CP160906
Coordinates   2327322..2329010 (-) Length   562 a.a.
NCBI ID   WP_354623558.1    Uniprot ID   -
Organism   Psychromonas sp. MME1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322322..2334010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2N10_RS10610 nagB 2322630..2323430 (+) 801 WP_354623553.1 glucosamine-6-phosphate deaminase -
  AB2N10_RS10615 nagA 2323470..2324612 (+) 1143 WP_354623555.1 N-acetylglucosamine-6-phosphate deacetylase -
  AB2N10_RS10620 - 2324677..2325906 (+) 1230 WP_354623556.1 ROK family transcriptional regulator -
  AB2N10_RS10625 pilC 2325971..2327203 (-) 1233 WP_369433828.1 type II secretion system F family protein Machinery gene
  AB2N10_RS10630 pilB 2327322..2329010 (-) 1689 WP_354623558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB2N10_RS10635 - 2329017..2329427 (-) 411 WP_354623559.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB2N10_RS10640 - 2329929..2330078 (-) 150 WP_354623560.1 hypothetical protein -
  AB2N10_RS10645 nhaD 2330079..2331521 (-) 1443 WP_354623561.1 sodium:proton antiporter NhaD -
  AB2N10_RS10650 - 2331640..2332002 (-) 363 WP_354623562.1 VanZ family protein -
  AB2N10_RS10655 - 2332013..2332372 (-) 360 WP_354623563.1 RNA-binding S4 domain-containing protein -
  AB2N10_RS10660 thiH 2332391..2333512 (-) 1122 WP_354623564.1 2-iminoacetate synthase ThiH -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61650.47 Da        Isoelectric Point: 5.1214

>NTDB_id=1022234 AB2N10_RS10630 WP_354623558.1 2327322..2329010(-) (pilB) [Psychromonas sp. MME1]
MATHHISGLASSLAARGLLPFAKLQQITSAAASQKKALVSYLVENNFISSVELAQFCEAEYGIPLLDAASLDLDEIPEKF
INQKLIEKHHALPVYVQQKTLYIAMSDPTNVAALEDFGFSFAIHTEALLVEESYLNKAIEKLLEDDVSSLGDMGDIDDIE
IDEGESRLDEAQGDSEDDAPIVKYINGILLDAVRKGASDLHFEPYEKKYRVRFRVDGILHEVATPPVNLANRFSARLKVM
SRLDIAERRLPQDGRIKLKISSKRNVDLRVSTLPTMWGEKIVMRILDSSAASLDIDRLGYSDEQKAKYLHALSKPQGMIL
ITGPTGSGKTVSLYTGLNILNTVERNISTAEDPVEINLAGINQVQINNKAGLTFASALRSFLRQDPDIVMVGEIRDLETA
EIGIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLARRLCPECKAVDEYSDKELQTIGFNHEQI
AAGITLYKPVGCAKCTDGYKGRVGIYEVMQMSDNISHIILNEGNAMQIANVAEAEGMDTLRQSALQQAIRGITSLKEVTR
VT

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1022234 AB2N10_RS10630 WP_354623558.1 2327322..2329010(-) (pilB) [Psychromonas sp. MME1]
ATGGCAACCCATCATATTAGCGGCTTGGCGTCTAGCCTCGCCGCTCGCGGTCTACTCCCCTTTGCGAAACTCCAACAGAT
AACCTCCGCTGCCGCTAGTCAAAAAAAAGCACTGGTTAGCTATTTGGTGGAAAATAATTTTATCTCTAGTGTTGAGCTGG
CTCAGTTCTGCGAAGCTGAATATGGTATTCCTCTATTAGACGCTGCCTCTCTGGACCTCGATGAGATTCCAGAAAAGTTT
ATTAATCAGAAACTCATAGAGAAACATCATGCGCTGCCCGTCTATGTGCAGCAGAAAACCCTCTATATCGCCATGTCCGA
CCCGACTAACGTCGCGGCTCTGGAAGATTTTGGCTTTAGTTTTGCCATCCATACCGAAGCCTTATTGGTGGAAGAGAGTT
ACCTTAATAAAGCCATTGAAAAGCTCTTAGAGGATGATGTCTCCTCCCTTGGTGATATGGGCGATATCGACGATATCGAA
ATTGACGAAGGTGAATCACGCCTTGACGAGGCGCAGGGCGACAGTGAAGATGATGCCCCGATCGTTAAATATATTAATGG
CATTCTGCTCGATGCGGTGCGCAAGGGCGCCTCGGATTTACACTTTGAGCCCTATGAAAAAAAATACCGCGTCCGTTTTC
GTGTTGATGGTATTTTACATGAGGTTGCTACGCCACCGGTTAATTTAGCGAACCGTTTTTCGGCGCGCCTTAAGGTGATG
TCGCGTTTGGATATTGCCGAGCGCCGTCTGCCCCAGGATGGTCGTATTAAGCTGAAAATATCGAGCAAACGTAATGTTGA
CCTGCGTGTTAGTACGCTACCTACCATGTGGGGCGAAAAAATCGTAATGCGTATTTTAGACTCCTCGGCTGCAAGCCTTG
ATATCGACCGTTTAGGTTACAGCGATGAACAAAAGGCGAAATATTTACATGCCCTGTCAAAACCGCAGGGGATGATCCTC
ATTACTGGGCCAACGGGCAGTGGTAAAACCGTTTCTCTCTATACGGGACTGAATATTCTCAATACCGTGGAGCGTAATAT
CTCAACAGCGGAAGATCCCGTGGAGATCAACCTTGCTGGTATTAACCAAGTACAGATCAATAATAAAGCAGGGCTGACCT
TTGCTTCAGCACTGCGCTCTTTTTTACGTCAAGATCCTGATATCGTGATGGTCGGTGAGATTCGAGATTTAGAGACCGCC
GAAATCGGCATTAAAGCCGCACAAACGGGCCATTTAGTGTTATCCACATTGCATACCAACTCGGCAGCAGAAACCTTAAC
GCGTTTGTTAAACATGGGCGTTCCCGCCTTTAACATCGCCTCTTCGGTATCCCTGATTATCGCACAACGTTTAGCGCGTC
GACTCTGCCCTGAGTGTAAAGCGGTAGATGAGTACAGTGACAAAGAGTTACAGACGATCGGCTTTAATCATGAGCAAATT
GCAGCAGGGATAACACTTTATAAACCTGTCGGTTGCGCTAAATGTACAGATGGGTATAAAGGACGTGTGGGTATTTATGA
AGTAATGCAGATGTCCGACAATATTAGTCACATCATCCTCAATGAAGGTAATGCGATGCAAATCGCCAATGTCGCCGAGG
CTGAAGGGATGGATACATTACGTCAATCGGCACTGCAACAGGCGATCAGAGGCATTACCAGTTTAAAAGAGGTGACCCGC
GTTACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.562

99.466

0.543
  pilB Legionella pneumophila strain ERS1305867

53.792

100

0.543
  pilB Acinetobacter baumannii D1279779

55.801

96.619

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

53.014

100

0.532
  pilB Vibrio campbellii strain DS40M4

52.205

100

0.527
  pilB Vibrio cholerae strain A1552

51.964

99.644

0.518
  pilF Neisseria gonorrhoeae MS11

48.593

94.84

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.286

99.644

0.391
  pilB/pilB1 Synechocystis sp. PCC 6803

38.406

98.221

0.377
  pilF Thermus thermophilus HB27

37.455

99.288

0.372

Multiple sequence alignment