Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABV589_RS10375 Genome accession   NZ_CP160398
Coordinates   2320152..2321852 (+) Length   566 a.a.
NCBI ID   WP_367085754.1    Uniprot ID   -
Organism   Pseudomonas sp. HOU2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315152..2326852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV589_RS10355 (ABV589_10355) - 2315328..2318276 (+) 2949 WP_367085751.1 DUF748 domain-containing protein -
  ABV589_RS10360 (ABV589_10360) - 2318276..2318572 (+) 297 WP_367086189.1 DUF2845 domain-containing protein -
  ABV589_RS10365 (ABV589_10365) - 2318835..2319191 (-) 357 WP_367085752.1 BON domain-containing protein -
  ABV589_RS10370 (ABV589_10370) pilA 2319488..2319925 (-) 438 WP_367085753.1 pilin Machinery gene
  ABV589_RS10375 (ABV589_10375) pilB 2320152..2321852 (+) 1701 WP_367085754.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABV589_RS10380 (ABV589_10380) pilC 2321855..2323072 (+) 1218 WP_096795218.1 type II secretion system F family protein Machinery gene
  ABV589_RS10385 (ABV589_10385) pilD 2323074..2323946 (+) 873 WP_367085755.1 A24 family peptidase Machinery gene
  ABV589_RS10390 (ABV589_10390) coaE 2323943..2324566 (+) 624 WP_086792846.1 dephospho-CoA kinase -
  ABV589_RS10395 (ABV589_10395) yacG 2324563..2324760 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  ABV589_RS10400 (ABV589_10400) - 2324767..2324982 (-) 216 WP_064382081.1 hypothetical protein -
  ABV589_RS10405 (ABV589_10405) - 2325048..2325737 (-) 690 WP_367085756.1 energy-coupling factor ABC transporter permease -
  ABV589_RS10410 (ABV589_10410) - 2325935..2326561 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62544.49 Da        Isoelectric Point: 5.9441

>NTDB_id=1020855 ABV589_RS10375 WP_367085754.1 2320152..2321852(+) (pilB) [Pseudomonas sp. HOU2]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHASGLEDMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKELYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIREGFPP
EQIGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1020855 ABV589_RS10375 WP_367085754.1 2320152..2321852(+) (pilB) [Pseudomonas sp. HOU2]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTCACAGGTGG
CCGAGATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTCGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCTTGTGGCGTCGTGGCAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACTGATGCCATCGAGAAATTCTTCGACACTCACGCCAGTGGTCTCGAAGACATGGCCGACGTCGATCTCGACGGC
GTGGATGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATTGGCGGCCTCGACGCCGACGACGCGCCGGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAGAGTGGCTCGTCGGACTTGCACTTCGAACCCTACGAAAAAAACTTCAGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGACGAATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGACATCTCGGAACGCCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCGAAGACCAA
GTCGATCGACTTCCGGGTCAACACCTTGCCGACCCTGTGGGGTGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAGATCGGCATCGATGCATTGGGTTACGAGCCGGCGCAGAAAGAGCTGTACATGGCAGCACTCAAGCAGCCGCAGGGC
ATGATTCTGGTAACCGGGCCTACGGGCTCGGGGAAAACCGTGTCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAACATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTTGCTCAGGCTCTGCGTTCGTTTCTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGTGACCTG
GAAACCGCCGAAATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGTCTGCACAACATGGGCATTCCCGGGTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCTCGCGAGACATTGATCAGGGAGGGTTTCCCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGATCACTGTAACGGCGGCTACAAGGGCCGCGTGGGGAT
TTACGAAGTGGTGAAGAACACCCCCGAGCTGCAACGGCTGATCATGGCCGAAGGCAACTCGTTGGAAATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.823

0.581
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

51.155

99.47

0.509
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.733

99.117

0.493
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

37.097

98.587

0.366

Multiple sequence alignment