Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABUE11_RS14680 Genome accession   NZ_CP160388
Coordinates   3106616..3108355 (+) Length   579 a.a.
NCBI ID   WP_367066044.1    Uniprot ID   -
Organism   Oryzisolibacter sp. LB2S     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101616..3113355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUE11_RS14650 - 3102015..3102899 (+) 885 WP_367066036.1 hypothetical protein -
  ABUE11_RS14655 - 3102996..3103478 (+) 483 WP_367066037.1 hypothetical protein -
  ABUE11_RS14660 - 3103541..3103933 (+) 393 WP_367066038.1 hypothetical protein -
  ABUE11_RS14665 - 3103937..3104956 (-) 1020 WP_367066039.1 IS30 family transposase -
  ABUE11_RS14670 - 3105479..3105661 (+) 183 WP_367066040.1 hypothetical protein -
  ABUE11_RS14675 - 3105667..3106008 (-) 342 WP_367066042.1 helix-turn-helix domain-containing protein -
  ABUE11_RS14680 pilB 3106616..3108355 (+) 1740 WP_367066044.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABUE11_RS14685 pilC 3108375..3109592 (+) 1218 WP_367066045.1 type II secretion system F family protein Machinery gene
  ABUE11_RS14690 - 3109592..3110473 (+) 882 WP_367066046.1 A24 family peptidase -
  ABUE11_RS14695 coaE 3110474..3111109 (+) 636 WP_367066047.1 dephospho-CoA kinase -
  ABUE11_RS14700 zapD 3111137..3111892 (+) 756 WP_367066048.1 cell division protein ZapD -
  ABUE11_RS14705 - 3111901..3112113 (+) 213 WP_367066049.1 DNA gyrase inhibitor YacG -
  ABUE11_RS14710 - 3112176..3112601 (-) 426 WP_367066050.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63230.56 Da        Isoelectric Point: 5.4577

>NTDB_id=1020761 ABUE11_RS14680 WP_367066044.1 3106616..3108355(+) (pilB) [Oryzisolibacter sp. LB2S]
MVAADSTPKAEQPVVLPGLARALMSVGKLNQKTAEEIYKKSLSGRSNFIAELIGASAVTSSDLAHTVSSIFGAPLLDLDA
LDRSRLPHDLLDPKLCQAYRVVVLSKRNNRLIVATADPTHQEAAEKIKFTTQMGVDWVIAEYDKLIQLVEATAKTSSETL
EGIVGGGDFEFGDISVEDAPEDSNDTAPAEVEDAPVVRFLHKMLLDAFNMRASDLHFEPYEHSYRVRFRIDGELREIASP
PIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEK
ERLLNAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALKAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPADI
PHASLIEAGYREEEIDGSWVTYRAVGCSACNNGYKGRVGIYQVMPISEEIQRIILRDGSAMDIAAQARLEGVRSLREAAL
YKAKIGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=1020761 ABUE11_RS14680 WP_367066044.1 3106616..3108355(+) (pilB) [Oryzisolibacter sp. LB2S]
ATGGTCGCCGCTGATAGCACGCCAAAGGCCGAGCAGCCTGTCGTCTTACCCGGTCTGGCACGGGCATTGATGTCTGTCGG
CAAGTTGAACCAGAAGACCGCAGAAGAAATTTACAAGAAGTCCCTGTCGGGCCGCTCGAATTTCATTGCGGAGTTGATAG
GAGCCAGCGCCGTCACGTCCAGCGATCTGGCCCACACCGTTTCTTCCATCTTTGGCGCCCCCCTGCTTGACCTGGATGCG
CTGGACCGCTCGCGCCTGCCGCACGACCTTCTCGACCCGAAGCTGTGTCAGGCCTACCGCGTGGTGGTGCTGAGCAAGCG
CAACAATCGTCTCATCGTTGCCACGGCAGATCCCACGCATCAGGAGGCCGCCGAGAAGATCAAGTTCACCACGCAGATGG
GGGTGGACTGGGTCATCGCCGAATATGACAAGCTGATCCAACTGGTCGAAGCGACGGCAAAGACCAGCAGCGAGACGCTG
GAAGGCATTGTCGGAGGCGGAGATTTCGAGTTTGGCGACATCTCAGTCGAGGACGCGCCGGAGGACAGCAACGACACGGC
TCCGGCAGAGGTCGAAGACGCACCGGTCGTCCGCTTCCTGCACAAGATGCTGCTGGATGCGTTCAACATGCGTGCGTCCG
ACCTGCATTTCGAACCCTACGAGCACAGCTACCGGGTGCGCTTTCGCATCGATGGCGAATTGCGCGAGATCGCGTCCCCA
CCCATTGCCATCAAGGAAAAGCTGGCCTCACGAATCAAGGTGATTTCACGCCTGGACATTTCCGAGAAGCGCGTACCTCA
GGATGGGCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGATTTTCGGGTGAGCACACTTCCCACTCTGTTCGGTG
AAAAGATCGTGATCCGCATTCTGGATCCGAGCAGCGCAAAACTCGGTATTGACGCTCTTGGCTATGAGCCGGAAGAGAAA
GAGCGTCTGCTGAACGCCATCAACCGGCCGTACGGCATGATTCTGGTGACCGGCCCCACGGGTTCCGGCAAGACGGTTTC
GCTGTACACCTGCCTGAACCTGCTCAACAAGCCGGGCGTGAATATCGCCACGGCCGAAGACCCATCGGAAATCAACCTGC
CGGGTGTGAATCAGGTCAACGTCAACGAGAAGGCGGGCCTGACTTTCGCCGCAGCGCTGAAGGCGTTTCTGCGTCAGGAT
CCCGACATCATCATGGTGGGTGAAATCCGGGACCTGGAGACCGCGGACATTTCCATCAAGGCAGCCCAGACGGGCCACCT
GGTGCTCTCCACGCTGCACACCAATGACGCGCCCACGACGCTCACGCGCATGCGCAACATGGGCATTGCGCCCTTCAATA
TCGCATCGAGCGTCATCCTGATCACCGCGCAGCGCCTCGCACGGCGCCTGTGTCCACAGTGCAAGGAACCGGCGGACATT
CCGCATGCCTCCCTGATCGAAGCTGGATACAGGGAAGAGGAAATTGACGGTTCCTGGGTGACCTATCGTGCTGTGGGTTG
CTCCGCCTGCAACAACGGATACAAGGGGCGGGTGGGCATATACCAGGTCATGCCGATATCCGAGGAAATTCAGCGCATCA
TCCTGCGCGACGGCAGCGCCATGGACATTGCAGCTCAGGCCCGCCTGGAGGGCGTGCGCTCGCTGCGCGAGGCGGCTCTG
TACAAAGCCAAGATTGGTCTTACCTCCCTGGAGGAGGTGCTGGCCGTGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)

Type II/IV secretion system protein (T2SSE)

(202-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.173

100

0.535
  pilB Acinetobacter baylyi ADP1

53.86

98.446

0.53
  pilF Neisseria gonorrhoeae MS11

53.274

97.582

0.52
  pilB Legionella pneumophila strain ERS1305867

49.381

97.582

0.482
  pilB Vibrio cholerae strain A1552

45.775

98.1

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

46.753

93.092

0.435
  pilB Vibrio campbellii strain DS40M4

43.902

99.136

0.435
  pilF Thermus thermophilus HB27

38.761

97.582

0.378

Multiple sequence alignment