Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABU188_RS00985 Genome accession   NZ_CP160327
Coordinates   229515..231194 (-) Length   559 a.a.
NCBI ID   WP_370469869.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224515..236194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU188_RS00955 (ABU188_12235) - 224843..225760 (-) 918 WP_370469866.1 ABC transporter ATP-binding protein -
  ABU188_RS00960 (ABU188_12240) yacG 225809..226006 (-) 198 WP_367421115.1 DNA gyrase inhibitor YacG -
  ABU188_RS00965 (ABU188_12245) zapD 226006..226758 (-) 753 WP_100140881.1 cell division protein ZapD -
  ABU188_RS00970 (ABU188_12250) coaE 226764..227378 (-) 615 WP_367421119.1 dephospho-CoA kinase -
  ABU188_RS00975 (ABU188_12255) pilD 227375..228226 (-) 852 WP_370469867.1 A24 family peptidase Machinery gene
  ABU188_RS00980 (ABU188_12260) pilG 228233..229465 (-) 1233 WP_370469868.1 type II secretion system F family protein Machinery gene
  ABU188_RS00985 (ABU188_12265) pilF 229515..231194 (-) 1680 WP_370469869.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABU188_RS00990 (ABU188_12270) recJ 231394..233091 (-) 1698 WP_370469870.1 single-stranded-DNA-specific exonuclease RecJ -
  ABU188_RS00995 (ABU188_12275) - 233088..234062 (-) 975 WP_367421121.1 hypothetical protein -
  ABU188_RS01000 (ABU188_12280) - 234111..235877 (-) 1767 WP_367421123.1 M61 family metallopeptidase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62257.84 Da        Isoelectric Point: 5.7880

>NTDB_id=1020457 ABU188_RS00985 WP_370469869.1 229515..231194(-) (pilF) [Snodgrassella alvi strain Acer10]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPMLLQSGVIDADKLATFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILENLGQESSSLLEGMDTVGDAEATGNN
AMSADDEDSENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTESVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEYAMKKAGFSDEDLAKDWTF
YRPVGCERCKGKGYKGRAGVYEVMPVSEEMQRIIMNNGNEVDISNQALKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1020457 ABU188_RS00985 WP_370469869.1 229515..231194(-) (pilF) [Snodgrassella alvi strain Acer10]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATGCTGTTGCAGTCTGGTGTCATCGATGCTGACAAACTGGCCACCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTGCCCATTTTCAAACGTGGAAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATACTGAATCTGGTCATTGTACGCGATGATCAGCTGCGTCAGATAC
TCGAAAATCTCGGACAGGAATCCTCCAGCCTACTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAAGACAGTGAAAATGGGCCTGTTGCCAGATTTGTATTCAAAGTACTGAATGATGCTCTGAA
CAGCGGCGCTTCAGATATACACTTCGAATTTTACGAAGAAATGGCACGAGTACGTTTTCGCATCGATGGTCAGTTACGTG
AAGTAGTGCGTATGCCACTGAATATTCGAAACCAGATTGCCTCCCGTATTAAAGTAATGGCTAAACTGGACATTTCAGAA
AAACGTATACCCCAAGATGGCCGAATTCAGATTCAATTCCGTAAAGGCGCTAAACCGATTGATTTTCGTGTCAGCACTCT
TCCGACCTTGTTCGGTGAAAAAATTGTGATGCGTATTCTGAATTCGGATGCTGCCACACTGAATATCGAACAGCTCGGTT
TCGAACCATTTCAGAAAGAATTACTGCTGGAAGCCATTCACCGGCCCTACGGTATGGTTCTAGTTACCGGCCCAACTGGT
TCCGGTAAAACCGTATCCCTCTATACCTGCTTAAATATTCTGAACACCGAAAGTGTGAACATTTCTACCGCTGAAGACCC
TGCAGAAATTAATTTACCCGGTATCAATCAGGTCAATGTAAATGATAAACAGGGACTGACATTTGCCGCAGCTCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGGGATTTAGAAACAGCCGATATTGCCATCAAAGCG
GCACAAACCGGCCATATGGTATTCTCTACCCTGCACACCAATAATGCGCCTGCTACCTTATCACGTATGCTGAATATGGG
GGTTGCTCCATTCAACATCGCAAGTTCAGTGAATCTGATTATGGCACAGCGTCTGGTTCGACGTTTATGTACCTGCAAAG
CACCAGCCGAACGGCCACCAGAGTATGCAATGAAAAAAGCTGGTTTCTCAGACGAAGATCTGGCCAAAGATTGGACATTT
TATCGGCCTGTAGGCTGTGAGCGCTGTAAAGGCAAAGGTTACAAGGGGCGCGCTGGTGTTTATGAAGTAATGCCAGTAAG
TGAAGAAATGCAACGTATAATCATGAACAACGGAAATGAAGTTGATATAAGTAATCAAGCACTTAAGGAAGGACTAGTCG
ATTTACGTCATGCTGGTCTACTTAAAGTAATGGAAGGCATCACCTCGCTTGACGAAATCCTCGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.929

100

0.691
  pilB Acinetobacter baylyi ADP1

50

100

0.508
  pilB Acinetobacter baumannii D1279779

52.239

95.886

0.501
  pilB Legionella pneumophila strain ERS1305867

47.979

100

0.488
  pilB Vibrio cholerae strain A1552

45.406

100

0.46
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

43.44

100

0.438
  pilF Thermus thermophilus HB27

39.963

96.243

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.038

95.17

0.381

Multiple sequence alignment