Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABUW22_RS03720 Genome accession   NZ_CP160326
Coordinates   839647..841326 (-) Length   559 a.a.
NCBI ID   WP_267408994.1    Uniprot ID   -
Organism   Snodgrassella alvi strain Acer01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 834647..846326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUW22_RS03690 (ABUW22_08710) - 834977..835894 (-) 918 WP_100140842.1 ABC transporter ATP-binding protein -
  ABUW22_RS03695 (ABUW22_08715) yacG 835944..836141 (-) 198 WP_267392005.1 DNA gyrase inhibitor YacG -
  ABUW22_RS03700 (ABUW22_08720) zapD 836141..836893 (-) 753 WP_100140881.1 cell division protein ZapD -
  ABUW22_RS03705 (ABUW22_08725) coaE 836899..837513 (-) 615 WP_367417838.1 dephospho-CoA kinase -
  ABUW22_RS03710 (ABUW22_08730) pilD 837510..838361 (-) 852 WP_367417839.1 A24 family peptidase Machinery gene
  ABUW22_RS03715 (ABUW22_08735) pilG 838368..839600 (-) 1233 WP_100151389.1 type II secretion system F family protein Machinery gene
  ABUW22_RS03720 (ABUW22_08740) pilF 839647..841326 (-) 1680 WP_267408994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABUW22_RS03725 (ABUW22_08745) recJ 841526..843223 (-) 1698 WP_370387724.1 single-stranded-DNA-specific exonuclease RecJ -
  ABUW22_RS03730 (ABUW22_08750) - 843220..844194 (-) 975 WP_367417840.1 hypothetical protein -
  ABUW22_RS03735 (ABUW22_08755) - 844243..846009 (-) 1767 WP_370387725.1 M61 family metallopeptidase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62245.82 Da        Isoelectric Point: 5.9508

>NTDB_id=1020437 ABUW22_RS03720 WP_267408994.1 839647..841326(-) (pilF) [Snodgrassella alvi strain Acer01]
MSVGLIRVLYKHQIIDAEKAEKLRQELEQKRPVIPMLLQSGVIDADKLASFLSKVFSIPYLNLELYNQSLVPQDLINLKI
MQQYICVPIFKRGNRLFLAVSDPTSIQHYQKIATDAGCILNLVIVRDDQLRQILENLGQESTSLLEGMDTVGDAEATGNN
AMSADDEEGENGPVARFVFKVLNDALNSGASDIHFEFYEEMARVRFRIDGQLREVVRMPLNIRNQIASRIKVMAKLDISE
KRIPQDGRIQIQFRKGAKPIDFRVSTLPTLFGEKIVMRILNSDAATLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKA
AQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKAPAERPPEFALKKAGFSDEDLAKEWTF
YRPVGCERCKGKGYKGRAGVYEVMPISDEMQRIIMNNGNEVDISQQAHKEGLVDLRHAGLLKVMEGITSLDEILATTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1020437 ABUW22_RS03720 WP_267408994.1 839647..841326(-) (pilF) [Snodgrassella alvi strain Acer01]
ATGAGTGTAGGATTGATTCGAGTTTTATATAAGCACCAGATTATCGATGCAGAAAAAGCTGAAAAACTGCGTCAGGAGCT
TGAGCAGAAACGTCCAGTTATACCGATGCTGTTGCAGTCTGGTGTCATCGATGCTGACAAACTGGCCAGCTTCCTGTCCA
AGGTATTCAGTATTCCTTACCTCAATCTGGAACTATATAACCAAAGCCTTGTACCGCAGGACTTAATCAATCTGAAAATC
ATGCAGCAATATATCTGCGTGCCCATTTTCAAACGTGGAAATCGTCTGTTTCTGGCTGTATCTGACCCCACTTCCATTCA
GCATTATCAGAAAATTGCCACAGATGCCGGCTGCATACTGAATCTGGTCATTGTACGCGATGATCAGCTTCGTCAGATAC
TAGAAAATCTCGGACAGGAATCCACCAGCCTGCTAGAAGGAATGGACACAGTTGGTGATGCTGAAGCAACAGGCAATAAT
GCCATGTCTGCCGATGATGAGGAGGGCGAAAACGGCCCTGTGGCCAGATTTGTATTCAAAGTTTTGAATGATGCACTCAA
CAGCGGCGCCTCAGATATTCACTTTGAATTTTACGAAGAAATGGCACGAGTACGTTTTCGTATTGACGGGCAGCTACGGG
AAGTAGTGCGTATGCCACTGAATATTAGAAACCAGATTGCCTCCCGTATTAAAGTGATGGCTAAACTGGACATTTCAGAA
AAACGTATACCTCAGGATGGCCGAATTCAGATTCAATTCCGCAAAGGCGCCAAACCGATTGATTTTCGTGTCAGCACCCT
TCCGACCCTGTTTGGTGAAAAAATTGTGATGCGTATTCTGAATTCGGATGCCGCTACCCTGAATATCGAACAGCTCGGCT
TCGAACCATTTCAGAAAGAATTACTGCTGGAAGCCATTCACCGGCCGTATGGTATGGTACTGGTTACCGGTCCAACCGGT
TCCGGTAAAACTGTATCCCTTTACACCTGCCTGAATATTCTGAATACCGACAGTGTGAACATTTCTACTGCAGAAGATCC
TGCGGAAATTAATTTACCCGGTATCAATCAGGTCAATGTAAATGATAAACAGGGGTTAACATTTGCCGCAGCTCTAAAAT
CTTTCCTACGTCAGGATCCAGACATCATCATGGTGGGGGAAATCCGTGATTTAGAAACGGCCGATATTGCCATCAAAGCA
GCACAAACCGGTCATATGGTATTTTCTACCCTGCACACCAATAATGCGCCTGCCACCTTATCACGTATGCTAAATATGGG
AGTTGCTCCATTCAACATCGCAAGTTCAGTGAATCTGATTATGGCACAGCGTTTGGTACGACGTTTATGTACCTGCAAAG
CACCAGCCGAACGGCCACCAGAATTCGCACTCAAAAAAGCCGGTTTCTCTGATGAAGATCTAGCTAAAGAATGGACTTTT
TATCGACCGGTAGGCTGTGAGCGCTGTAAAGGCAAAGGTTACAAGGGGCGCGCTGGTGTTTATGAAGTAATGCCTATCAG
TGATGAAATGCAGCGAATCATCATGAACAATGGTAATGAAGTCGATATCAGTCAGCAGGCGCATAAAGAAGGACTGGTCG
ATTTGCGTCATGCTGGTCTGCTTAAAGTTATGGAAGGCATCACTTCGCTTGACGAAATTCTAGCCACCACCAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.107

100

0.692
  pilB Acinetobacter baylyi ADP1

50.528

100

0.513
  pilB Acinetobacter baumannii D1279779

51.583

96.064

0.496
  pilB Legionella pneumophila strain ERS1305867

47.979

100

0.488
  pilB Vibrio cholerae strain A1552

45.583

100

0.462
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

43.262

100

0.436
  pilF Thermus thermophilus HB27

40.335

96.243

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

95.17

0.385

Multiple sequence alignment